Fatty acids, C16 and C18-unsaturated, methyl esters, chlorinated

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

January 31, 2022

Reliability:

1 (reliable without restriction)

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes

Specific details on test material used for the study:

The average log Kow varied from 5.4 to 6.0.
The water solubility was from 0.8 mg L-1 for the congener containing 4 Cl atoms to 0.3 mg L-1 for the congener with 9 Cl atoms.

Analytical monitoring:

yes

Vehicle:

no

Details on test solutions:

WAF preparation:
Loading rates (nominal concentrations): A stock elution was prepare using a nominal concentration of 1000 mg/L. After adding salts, it was then diluted to get all the tested concentrations.
Sample weight: Direct addition of 1000 mg (actial weight = 999.8 mg) per liter for the stock elution
Ambient temperature 20°C. Orbital shaker 100 rpm- 7 days.
At the end of shaking, the eluate is slightly turbid and whitish, partly jellified and hard to filter. After longer decantation a thin a whitish layer settles on the bottom of the container.
Filtration: With vacuum filtration apparatus and membranes with 0.45 µm pores of cellulose nitrate. After filtrations, the stock eluate was added with the salts and dilutions were prepared using the cultivation medium. Oxygenation of the eluate and its dilutions followed, via air insufflation for 30’ prior to starting the test and acclimatation to test temperature (20 °C).

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

The fishes came from a single group of similar length (1-2 cm) and age.

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Hardness:

160 mg CaCO3/L

Test temperature:

23 °C

pH:

7.5

Dissolved oxygen:

≥ 60% (i.e. 5.14 mg/L at 23 °C and 1 atm)

Salinity:

0.13 ‰

Nominal and measured concentrations:

Limit test at 1000 mg/L

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 0.8 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat. (dissolved fraction)

Remarks:

The WAF of different congeners C19Cl4 - C19Cl9 has been determined, the mean concentration was 0.8 mg/L. Specifically, the concentration in C19Cl4 was 0.9, in C19Cl5 0.07, in C19Cl6 0.03, in Cl19Cl7 0.02, in C19Cl8 0.02, in C19Cl9 0.03.

Basis for effect:

mortality (fish)

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Validity criteria fulfilled:

yes

Conclusions:

After 96h of exposure at the limit concentration of 1000 mg/L, the sample does not induce the mortality of any of the seven Zebrafish under test (0/7), that is an acceptable value for the limit test. Overall, LC50 @ 96h > 1000 mg/L.

Executive summary:

This technical report summarizes all the data collected during the study: the exposure conditions of the fish at the tested sample limit concentration and the calculation of results. When using vertebrates for scientific purposes, as forced by the EU regulations, the 3 Rs rule must be obeyed. It includes three principles: Replacement, Reduction, and Refinement. Reduction refers to the use of methods that enable researchers to obtain comparable levels of information from fewer animals, or to obtain more information from the same number of animals. Whenever possible, for eco-tests on fishes, the limit test (one single concentration) is to be preferred to the serial concentrations test (at least five concentrations). Seven Zebrafishes, with a recommended total length of 1-2 cm, are exposed to the sample at the chosen limit concentration in breeding water, for a period of 96 hours. Mortality is recorded at 24, 48, 72 and 96 hours and compared with the negative control.

Description of key information

The substance has a nominal LC50 > 1000 mg/L. 

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Dose descriptor:

LC50

Effect concentration:

> 1 000 mg/L