Fatty acids, C16 and C18-unsaturated, methyl esters, chlorinated

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

January 31, 2022

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

WAF preparation:
Loading rates (nominal concentrations): A stock elution was prepare using a nominal concentration of 1000 mg/L. After adding salts, it was then diluted to get all the tested concentrations.
Sample weight: Direct addition of 1000 mg (actial weight = 999.8 mg) per liter for the stock elution
Ambient temperature 20°C. Orbital shaker 100 rpm- 7 days.
At the end of shaking, the eluate is slightly turbid and whitish, partly jellified and hard to filter. After longer decantation a thin a whitish layer settles on the bottom of the container.
Filtration: With vacuum filtration apparatus and membranes with 0.45 µm pores of cellulose nitrate. After filtrations, the stock eluate was added with the salts and dilutions were prepared using the cultivation medium. Oxygenation of the eluate and its dilutions followed, via air insufflation for 30’ prior to starting the test and acclimatation to test temperature (20 °C).

Analytical monitoring:

not specified

Details on sampling:

The test containers were filled with 50 ml of the oxygenated sample dilutions or stock solution. The sole breeding medium was used as a negative control. The Daphnia specimens were then placed in the test vessels. For each concentration and for the control, 20 animals were used, divided into four groups of five animals each.

Vehicle:

no

Test organisms (species):

Daphnia magna

Test type:

not specified

Water media type:

other: distillated water ASTM Type III

Limit test:

no

Total exposure duration:

48 h

Hardness:

170 mg CaCO3/L

Test temperature:

20°C

pH:

7.3

Details on test conditions:

Tested concentrations:
• 50 mg/L
• 100 mg/L
• 250 mg/L
• 500 mg/L
• 1000 mg/L (stock eluate)*

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 0.7 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat. (dissolved fraction)

Remarks:

The WAF of different congeners C19Cl4 - C19Cl9 has been determined, the higher concentration determined was 0.7 mg/L for congener C19Cl4. This has been used as worst case for EC50 determination.

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Validity criteria fulfilled:

yes

Conclusions:

For the sample it was not possible to calculate the EC50 value via the PROBIT software, since a limited number of Daphnia specimen was immobilized after 48h at any of the tested concentrations, never reaching 50%.
The maximum number of immobilized animals is 2 out of 20 (10%). Overall, EC50 > 1000 mg/L.

Executive summary:

Young daphnids, aged less than 24 hours at the start of the test, were exposed to the test substance in a range of concentrations, for a period of 48 hours. The immobilisation was recorded at 24 hours and 48 hours and compared with the control. The results were analysed to assess the EC50 at 48h.

Overall, EC50 > 1000 mg/L.

Description of key information

The substance has a nominal EC50 > 1000 mg/L. 

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Dose descriptor:

EC50

Effect concentration:

> 1 000 mg/L