Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Currently viewing:

Administrative data

Link to relevant study record(s)

short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
January 31, 2022
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
according to guideline
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes (incl. QA statement)
Specific details on test material used for the study:
WAF preparation:
Loading rates (nominal concentrations): A stock elution was prepare using a nominal concentration of 1000 mg/L. After adding salts, it was then diluted to get all the tested concentrations.
Sample weight: Direct addition of 1000 mg (actial weight = 999.8 mg) per liter for the stock elution
Ambient temperature 20°C. Orbital shaker 100 rpm- 7 days.
At the end of shaking, the eluate is slightly turbid and whitish, partly jellified and hard to filter. After longer decantation a thin a whitish layer settles on the bottom of the container.
Filtration: With vacuum filtration apparatus and membranes with 0.45 µm pores of cellulose nitrate. After filtrations, the stock eluate was added with the salts and dilutions were prepared using the cultivation medium. Oxygenation of the eluate and its dilutions followed, via air insufflation for 30’ prior to starting the test and acclimatation to test temperature (20 °C).
Analytical monitoring:
not specified
Details on sampling:
The test containers were filled with 50 ml of the oxygenated sample dilutions or stock solution. The sole breeding medium was used as a negative control. The Daphnia specimens were then placed in the test vessels. For each concentration and for the control, 20 animals were used, divided into four groups of five animals each.
Test organisms (species):
Daphnia magna
Test type:
not specified
Water media type:
other: distillated water ASTM Type III
Limit test:
Total exposure duration:
48 h
170 mg CaCO3/L
Test temperature:
Details on test conditions:
Tested concentrations:
• 50 mg/L
• 100 mg/L
• 250 mg/L
• 500 mg/L
• 1000 mg/L (stock eluate)*
48 h
Dose descriptor:
Effect conc.:
> 0.7 mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat. (dissolved fraction)
The WAF of different congeners C19Cl4 - C19Cl9 has been determined, the higher concentration determined was 0.7 mg/L for congener C19Cl4. This has been used as worst case for EC50 determination.
Key result
48 h
Dose descriptor:
Effect conc.:
> 1 000 mg/L
Nominal / measured:
Conc. based on:
test mat.
Basis for effect:
Validity criteria fulfilled:
For the sample it was not possible to calculate the EC50 value via the PROBIT software, since a limited number of Daphnia specimen was immobilized after 48h at any of the tested concentrations, never reaching 50%.
The maximum number of immobilized animals is 2 out of 20 (10%). Overall, EC50 > 1000 mg/L.
Executive summary:

Young daphnids, aged less than 24 hours at the start of the test, were exposed to the test substance in a range of concentrations, for a period of 48 hours. The immobilisation was recorded at 24 hours and 48 hours and compared with the control. The results were analysed to assess the EC50 at 48h.

Overall, EC50 > 1000 mg/L.

Description of key information

The substance has a nominal EC50 > 1000 mg/L. 

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Dose descriptor:
Effect concentration:
> 1 000 mg/L

Additional information