ethyl 5,5-diphenyl-2-isoxazoline-3-carboxylate

Toxicity to terrestrial arthropods

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

toxicity to terrestrial arthropods: short-term

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

other: Only summary report available

GLP compliance:

no

Application method:

contact

Test organisms (species):

other: entomology screening species

Animal group:

other: arthropods, terrestrial

Details on test organisms:

TEST ORGANISMS
Spodoptera littoralis (Egyptian cotton leafworm)
Aphis fabae (Black bean aphid)
Trialeurodes vaporariorum (Greenhouse whitefly)
Nilaparvata lugens (Brown plant hopper)
Oncopeltus fasciatus (Large milkweed bug)
Diabrotica undecimpunctata (Southern corn rootworm)
Tetranychus urticae (Two spotted spider mite)
Blattella germanica (German cockroach)
Musca domestica (Housefly)

Study type:

laboratory study

Table: Primary screening results: LC 90/100 ppm a.i.

Species	Treated stage	AE F122006 WP 20 (ppm a.i.)
Spodoptera littoralis	larvae (L4)	> 1000
Aphis fabae	all stages	> 1000
Trialeurodes vaporariorum	eggs (3 d)	> 1000
Nilaparvata lugens	larvae (L3)	> 1000
Oncopeltus fasciatus	eggs (2 d)	> 1000
Diabrotica undecimpunctata	larvae (L2)	> 1000
Tetranychus urticae	all stages	> 1000
Blattella germanica	larvae (L4)	> 1000
Musca domestica	adults (1 d)	> 1000

Reference 2

Endpoint:

toxicity to terrestrial arthropods: short-term

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

29 Jun - 19 Oct 1998

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

other: EPPO Guideline No. 170 (1992)

GLP compliance:

yes

Application method:

contact

Analytical monitoring:

no

Vehicle:

yes

Details on preparation and application of test substrate:

- Method of test material application: single topical dose
- Body part: ventral thorax
- Volume of test solution applied: 1.0 μL
- Controls: 1.0 μL acetone
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): acetone

Test organisms (species):

Apis mellifera

Animal group:

Hymenoptera (honeybees)

Details on test organisms:

TEST ORGANISM
- Common name: Honey bee
- Source: The bees used for testing were taken from colonies on the land of Hoechst Schering AgrEvo GmbH, Frankfurt am Main, Germany.
- Disease free: yes

Study type:

laboratory study

Limit test:

yes

Total exposure duration:

72 h

Test temperature:

25 - 26 °C

Humidity:

68- 80 %

Photoperiod and lighting:

constant darkness during the test period (Observations were made under light.)

Details on test conditions:

TEST SYSTEM
- Test container / cage (material, size): wire mesh cages, which were closed at both ends with a cork
Number of test series: 3 ( Control, test - and reference substance)
Number of test concentrations: Test compound: 2; Reference substance: 5
Number of replicates: 5
Number of bees per replicate: 10

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Dead bees were recorded 24, 48 and 72 hours after application of the test or reference substance.

Nominal and measured concentrations:

50.4 and 100.7 μg technical substance/bee

Reference substance (positive control):

yes

Remarks:

Triazophos: 40.9 %w/w

Duration:

72 h

Dose descriptor:

LD50

Effect conc.:

> 100.7 other: μg technical substance per bee

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: highest test concentration

Details on results:

- No mortalities were observed during the test
- No unforeseen circumstances were observed, which may have affected the quality or integrity of this study.
- The study was performed as a limit test, but two concentrations were tested

Results with reference substance (positive control):

- Results: See "Any other information on results incl. tables", LD50 not reported
- Although the values for the reference substance were slightly above normal they remain within the range of biological variation

Table 1: Number of dead bees for reference substance

μg product / bee	Total number of dead bees after:
	24 h	48 h	72 h
0.1	0	0	0
0.2	2	5	5
0.3	39	39	39
0.4	48	48	48
0.5	47	47	47

Reference 3

Endpoint:

toxicity to terrestrial arthropods: short-term

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

02 Sep 1998 - 18 Feb 1999

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

other: EPPO Guideline No. 170

GLP compliance:

yes

Application method:

oral

Details on preparation and application of test substrate:

- Details of food source: The diet paste was prepared by HMR Deutschland GmbH, ProTox, Analytik, 65795 Hattersheim and consisted of powdered sugar, honey and deionized water.
- Method of feeding during study: through feeding tube

Test organisms (species):

Apis mellifera

Animal group:

Hymenoptera (honeybees)

Details on test organisms:

TEST ORGANISM
- Common name: honey bee
- Source: The bees used for testing were taken from colonies on the land of Hoechst Schering AgrEvo GmbH, Frankfurt am Main.
- Disease free: yes

Study type:

laboratory study

Limit test:

no

Total exposure duration:

72 h

Test temperature:

25-26 °C

Humidity:

65-73%

Photoperiod and lighting:

Constant darkness during the experimental phase (Observations were made under light.)

Details on test conditions:

TEST SYSTEM
- Test container / cage (material, size): Cylindrical test cages of wire mesh screening (12-13 cm high and 5 cm diameter), closed at each end with a cork plug.
- Number of test series: 3 ( Control, test - and reference substance)
- Number of test concentrations: Test compound: 3
- Reference substance: 5
- Number of replicates: 5
- Number of bees per replicate: 10

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Dead and damaged bees were recorded 24, 48 and 72 hours after application.

FOOD CONSUMPTION (if oral study)
- Amount of treated diet consumed per group: see "Any other information on results incl. tables" below

Nominal and measured concentrations:

Nominal: 0.01; 0.1 and 1% technical substance in diet.
Based on the quantity of food actually consumed during the 5 hr feeding period, the mean
measured dose rates to which the bees were exposed were equivalent to 1.72, 15.04 and
151.96 ug technical substance/bee.

Reference substance (positive control):

yes

Remarks:

Triazophos 40.9% w/w

Duration:

48 h

Dose descriptor:

LD50

Effect conc.:

> 151.96 µg per animal

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: highest test concentration (1% technical substance)

Duration:

72 h

Dose descriptor:

LD50

Effect conc.:

> 151.96 µg per animal

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: highest test concentration (1% technical substance)

Results with reference substance (positive control):

- Results: see Table 2 under "Any other information on results incl. tables", LD50 not reported

Table 1: Mortality and food consumption with test substance:

% a.i. in diet	Mean net food consumption per bee (g)	Ingested dose per bee (μg a.i.)	Total number of dead bees after:
			24 h	48 h	72 h
Control	0.0164	-	1	1	3
0.01	0.0172	1.7154	0	0	0
0.10	0.0150	15.0380	2	2	4
1.00	0.0152	151.9600	0	0	1

Table 2: Mortality and food consumption with reference substance:

% a.i. in diet	Mean net food consumption per bee (g)	Ingested dose per bee (μg a.i.)	Total number of dead bees after:
			24 h	48 h	72 h
Control	0.0164	-	1	1	3
0.00012	0.0284	0.03404	1	2	2
0.00024	0.0238	0.05705	8	8	8
0.00048	0.0212	0.10180	21	21	21
0.00096	0.0190	0.18282	41	42	42
0.00192	0.0215	0.41368	50	50	50

Additional information

Two acute studies with honeybees (Apis mellifera) are available according to EPPO Guideline No. 170 (adopted 1992). In the contact toxicity test, two concentrations, 50.4 and 100.7 µg/bee, were applied. In the oral toxicity test, 0.01, 0.1 and 1% of the test substance in the diet was administered. Neither of the tests revealed mortality at the highest concentration investigated. Thus, it is concluded that the LD50 is > 100.7 µg/bee in the contact test and >151.96 µg/bee in the oral toxicity test.