A mixture of cis- and trans-cyclohexadec-8-en-1-one

Bioaccumulation: aquatic / sediment

Link to relevant study record(s)

Reference

Endpoint:

bioaccumulation in aquatic species: fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1999-07-05 to 1999-07-16

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study without detailed documentation

Qualifier:

according to guideline

Guideline:

other: Bioconcentration: Flow-through Fish Test"; Council Directive 67/548/EWG C.13

Version / remarks:

1998

GLP compliance:

yes

Radiolabelling:

no

Details on sampling:

Analysis of test and control media as well as fishsamples at 5 h, 24 h, 48 h, 72 h, 96 h and 168 h after test start during the uptake phase were performed. After the beginning of the depuration phase (174 h) analysis was carried out in water and in fish samples at 192 h, 216 h, 240 h and 264 h.

Vehicle:

no

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebra fish
- Source: Bio International B.V. (Netherlands)
- Length at study initiation: 2.5-3.5 cm
- Feeding during test
- Food type: Tetra Min fish food, ground;
- Amount: 2 % of body weight per day
- Frequency: twice per day

Route of exposure:

aqueous

Test type:

flow-through

Water / sediment media type:

natural water: freshwater

Total exposure / uptake duration:

174 h

Total depuration duration:

89 h

Hardness:

12.1 °dH

Test temperature:

Uptake phase:
control: 21.9-23.6 °C;
Test solution: 22.1-24.2 °C;

Depuration phase:
control: 21.6-23.8 °C;
Test solution: 22.5-23.7 °C

pH:

Uptake phase:
control: 7.6-7.9;
Test solution: 7.6-7.8;

Depuration phase:
control: 7.8-8.1;
Test solution: 7.8-8.1

Dissolved oxygen:

Uptake phase:
control: 83.5-93.4% of saturation
Test solution: 86.3-96.8% of saturation

Depuration phase:
control: 93.1-103.6% of saturation
Test solution: 92.8-107.1% of saturation

Details on test conditions:

TEST SYSTEM
- Test vessel (size):600 x 295 x 290 mm;
- Type: open
- Fill volume: 40 L
- Aeration: yes
- Type of flow-through: proportional diluter
- No. of organisms per vessel: 100

OTHER TEST CONDITIONS
- Photoperiod:16 hours light / 8 hours dark

Nominal and measured concentrations:

Nominal concentration: 7.0 µg/L;

Measured concentration:
During the uptake phase in Test medium: 0.7-1.9 µg/L; During the uptake phase in Fish: 95-346 µg/kg;
During the depuration phase in Test medium: 0.7-1.9 µg/L; During the depuration phase in Fish: 98-35-15-13 µg/kg on day 1, 2, 3, 4.

Lipid content:

4.2 %

Time point:

start of exposure

Lipid content:

6.1 %

Time point:

end of exposure

Key result

Conc. / dose:

7 µg/L

Temp.:

23 °C

pH:

7.7

Type:

BCF

Value:

244 dimensionless

Basis:

whole body w.w.

Calculation basis:

steady state

Rate constant:

overall depuration rate constant (d-1)

Value:

-0.035

Rate constant:

overall uptake rate constant (L kg-1 d-1)

Remarks on result:

not determinable

Remarks:

The rapid achievement of steady-state conditions during the uptake phase did not allow for a determination of the uptake rate constant (k1).

Details on results:

Not reported in details

Validity criteria fulfilled:

yes

Conclusions:

The bioconcentration factor (BCF) of the test item was determined to be 244 at steady state.

Executive summary:

The study on the bioaccumulation potential was performed according to Council Directive 67/548/EWG C.13, "Bioconcentration: Flow-through Fish Test" (1998) and GLP. The test was carried out with Zebra fish under a flow through condition with a concentration level of 7 µg/L. The durations of uptake phase and depuration phase were 174 hours and 89 hours, respectively. The concentration of the test item was determined via GC/MS method. The bioconcentration factor (BCF) of the test item was determined to be 244 at steady state.

Description of key information

A study on the bioaccumulation potential is available, which was performed according to Council Directive 67/548/EWG C.13, "Bioconcentration: Flow-through Fish Test" (1998) and GLP. The test was carried out with Zebra fish under a flow through condition with a concentration level of 7 µg/L. The durations of uptake phase and depuration phase were 174 hours and 89 hours, respectively. The concentration of the test item was determined via GC/MS method. The bioconcentration factor (BCF) of the test item was determined to be 244 at steady state.

Key value for chemical safety assessment

BCF (aquatic species):

244 dimensionless