2-(2-oxopyrrolidin-1-yl)butyramide

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2004

Report date:

2004

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Reference

Name:

Unnamed

Type:

Constituent

Test material form:

solid: particulate/powder

Remarks:

migrated information: powder

Details on test material:

Batch: C02421 -1620
Purity: 98.6%

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

The study was performed with young daphnids of a clone of the species Daphnia magna Straus. A clone of this species was originally supplied by the University of Shefield/UK in 1992, defined by the supplier as clone 5. Since that time, the clone has been bred in the laboratories of RCC in reconstituted water of the quality identical to the water quality used in the tests (regarding pH, main ions, and total hardness) and under temperature and light conditions identical to those of the tests. At the start of the test, the organisms used in the test were 6–24 hours old and were not first brood progeny.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Hardness:

2.5 mmol/L (= 250.0 mg/L) as CaCOs

Test temperature:

20 °C during the test period. The test was performed in a temperature-controlled room (room temperature continuously monitored)

pH:

7.8 - 7.9

Dissolved oxygen:

The test water was aerated prior to the start of the study until oxygen saturation was reached. During the test period, the test water was not aerated.

Nominal and measured concentrations:

Reconstituted test water analytical grade salts were dissolved in purified water to obtain the following nominal concentrations:
CaCl2 x 2 H20 : 2.0 mmol/L (= 294.0 mg/L)
MgSO4 X 7 H20 : 0.5 mmol/L (= 123.0 mg/L)
NaHCO3 : 0.75 mmol/L (= 65.0 mg/L)
KCI : 0.075 mmol/L (= 5.8 mg/L)
Alkalinity 0.8 mmol/L

Details on test conditions:

A 16-hour light to 8-hour dark photoperiod (with a 30 minute transition period). Light intensity during the light period was between 460 and 670 Lux.

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

>= 100 mg/L

Effect concentrations 2

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

>= 100 mg/L

Applicant's summary and conclusion

Conclusions:

The 48-hour NOEC (highest concentration tested without toxic effects after the exposure period of 48 hours) and the 48-hour ECO of ucb 6474 to Daphnia magna were determined to be at least 100 mg/L.