Registration Dossier

Administrative data

Endpoint:
adsorption / desorption: screening
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
other: Body responsible for the test
Title:
Unnamed
Year:
2007
Report date:
2007

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
EU Method C.19 (Estimation of the Adsorption Coefficient (KOC) on Soil and Sewage Sludge Using High Performance Liquid Chromatography (HPLC))
Version / remarks:
Directive 2001/59/EC. Published in O.J. L 225, 2001
GLP compliance:
yes
Type of method:
HPLC estimation method
Media:
soil/sewage sludge

Test material

Reference
Name:
Unnamed
Type:
Constituent

Study design

Test temperature:
25 degree Celsius (C)

HPLC method

Details on study design: HPLC method:
EQUIPMENT
- Apparatus: Liquid chromatograph
- Type: Shimadzu LC-2010AHT with UV/VIS detector
- Type, material and dimension of analytical (guard) column: ZORBAX CN, 5 micrometrs, 150 x 4.6 mm


MOBILE PHASES
- Type: 550 mL 0.01 M citrate-buffer pH=6.1 was mixed with 450 mL of methanol
- Experiments with additives carried out on separate columns: no
- pH: 6,0
- Solutes for dissolving test and reference substances: methanol


DETERMINATION OF DEAD TIME
- Method: by inert substances which are not retained by the column


REFERENCE SUBSTANCES
- Identity: acetanilide, aniline, atrazin, benzamide, 2,5-dichloraniline, phenyl benzoate, 2-nitrobenzamide


DETERMINATION OF RETENTION TIMES
- Quantity of test substance introduced in the column: 20 micrometrs
- Quantity of reference substances: 10 micrometrs


REPETITIONS
- Number of determinations: 4 times


EVALUATION
- Calculation of capacity factors k: k=(retention time of test compound/ dead time) - 1
- Determination of the log Koc value: log Koc = (log capacity factor - intercept of calibration line)/ slope of the calibration line

Batch equilibrium or other method

Details on test conditions:
Deviations
The HPLC method is only applicable to chemical substances with log Koc values ranging from 1.5 to 5.0. The test substance does not meet this
requirement, at all. This leads to the retention time of the test substance much lower than that of any reference compound. A linear extrapolation is
only applicable instead of an interpolation.
It can be concluded with an assurance, that a value of the log Koc of test substance is lower than the lowest value used for calibration, however the
resulting value should be only considered as an informative one.

Results and discussion

Adsorption coefficient
Type:
log Koc
Value:
1.3

Results: HPLC method

Details on results (HPLC method):
- Retention times of reference substances used for calibration: see below under remarks
- Graph of regression line attached
- Average retention data for test substance: 1.4045 min

Results: Batch equilibrium or other method

Adsorption and desorption constants:
Adsorbtion coefficient of 3-(2,2,2-Trimethylhydrazinium)methylpropionate bromide on soil:
log Koc< 1.3 (-2.1 ± 2.6) (pH = 6.1, 25 degree Celsius (C))

Adsorbtion coefficient of 3-(2,2,2-Trimethylhydrazinium)methylpropionate bromide on sewage sludge:
log Koc < 1.5 (-2.2 ± 2.7) (pH = 6.1, 25 degree Celsius (C)).
Recovery of test material:
The test substance is not recovered

Any other information on results incl. tables

Retention times of reference substances used for calibration:

 Reference compound  tR I tR II   tR III tR   IV
 Acetanilide  2,802 2,800   2,802  2,800
 Benzamide  2,407 2,406  2,407  2,406 
 2 -Nitrobenzamide 2,317  2,317 2,317   2,316
 Atrazin  3,996  3,994 3,994   3,992
Aniline   2,649  2,650  2,650  2,648
 2,5 -Dichloroaniline  5,098  5,096  5,096  5,093
 Phenyl benzoate  9,115  9,110  9,108  9,103

Applicant's summary and conclusion