Registration Dossier

Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: well documented publication which meets basic scientific principles.

Data source

Reference
Reference Type:
publication
Title:
Actual survey on TLm (median tolerance limit) values of environmental pollutants, especially on amines, nitrilens, aromatic nitrogens compounds and artificial dyes
Author:
Tonogai Y et al.
Year:
1982
Bibliographic source:
Journal of Toxicological Sciences 7:193-203

Materials and methods

Principles of method if other than guideline:
The TLm (Median tolerance limit) was carried out according to Japanese industrial standards (Japanese Industrial Standards Comittee, 1971).
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
I- Name of test material (as cited in study report): p-aminoazobenzene
- Analytical purity: not stated,

Sampling and analysis

Analytical monitoring:
no

Test solutions

Details on test solutions:
Chemical substances were dissolved in water and neutralized, if necessary. Some Substances were dissolved in 1 ml ethanol, and then diluted with water.

Test organisms

Test organisms (species):
Oryzias latipes
Details on test organisms:
Fish of the same age (ca. 2 cm length; 0.2 g weight) for each experiment.

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Test temperature:
25°C

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
0.7 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
24 h
Dose descriptor:
LC50
Effect conc.:
1.7 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality

Applicant's summary and conclusion