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EC number: 432-770-2
CAS number: 139189-30-3
A study was performed to assess the acute toxicity of the test item to the earthworm(Eisenia foetida)in an artificial soil. The method followed that described in the OECD Guidelines for Testing of Chemicals (1984) No 207 "Earthworm, Acute Toxicity Tests" and Commission Regulation (EC) No. 440/2008. Study performed in compliance with the Principles of Good Laboratory Practice (GLP) and reported with a valid GLP certificate.
Following a preliminary range-finding test, 60 earthworms (six replicates of 10 worms) were exposed to a single concentration of 1000 mg/kg of soil for a period of 14 days at a temperature of 19°C to 23°C. The number of mortalities were determined after 7 and 14 days exposure. A positive control using chloroacetamide, conducted approximately every 6 months, is reported for reference purposes.
The 14-Day LC50 for the test item to earthworms(Eisenia foetida)based on the nominal test concentration was greater than 1000 mg/kg. The No Observed Effect Concentration was equal to or greater than 1000 mg/kg.
The result of the positive control gave a 14-Day LC5o for chloroacetamide of 43 mg/kg with 95% confidence limits of 41 - 45 mg/kg. The No Observed Effect Concentration was 18 mg/kg.
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