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EC number: 403-030-6 | CAS number: 137398-54-0 ROBAC AS/100
Long-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
Description of key information
Expressed as nominal concentration of the test substance, the lethal effect of ROBAC AS100 on Daphnia magna is as following:
The 21 days EC50 of the test substance to Daphnia magna was higher than the saturated solution concentration under the test condition (measured concentration is less than LOD (LOD=1.12 ng/ml)).
Key value for chemical safety assessment
Additional information
Introduction
According to OECD, Guidelines for the Testing of Chemicals, 2008, 211 Daphnia magna Reproduction Test, the influence of the test substance ROBAC AS100 to Daphnia magna was determined in a 21 days reproduction test. One treatment group and one control group were included in definitive test.
Method
Base on the information provided by the sponsor, physical and chemical nature of the test substance, the test substance is poorly water-soluble. A pre-test was performed before the definitive test and the treatment group was prepared as the saturated solution under the test conditions.
Results
The mortality of the parent animals was 10% at the end of the test in control group. No aborted eggs and dead offspring were observed during the test. The mean number of live offspring produced per parent animal surviving at the end of the test in control group was 110. The test is valid.
There were no observed inhibition on production of Daphnia magna during this test. The concentrations of the test substance in the
test solutions were analyzed using HPLC method. In the definitive test, the measured concentration of test substance was less than Limit of detection (LOD). Under test conditions, the 21 days EC50 of the test substance to Daphnia magna was higher than the concentration of the saturated solution of the test condition (measured concentration is less than LOD (LOD=1.12 ng/ml)).
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