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EC number: 258-054-8 | CAS number: 52628-25-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
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- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
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- Nanomaterial pour density
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- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
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- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Repeated dose toxicity: oral
Administrative data
- Endpoint:
- short-term repeated dose toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- Not reported
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Study well documented, meets generally accepted scientific principles, acceptable for assessment.
- Justification for data waiving:
- other:
Cross-referenceopen allclose all
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to other study
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 1 992
Materials and methods
- Principles of method if other than guideline:
- The test material was orally administered to Wistar rats daily for a period of 28 d. Animals were sacrificed after test material treatment to determine hematological indices.
- GLP compliance:
- no
- Limit test:
- no
Test material
- Reference substance name:
- Zinc chloride
- EC Number:
- 231-592-0
- EC Name:
- Zinc chloride
- Cas Number:
- 7646-85-7
- IUPAC Name:
- zinc dichloride
- Details on test material:
- - Name of test material (as cited in study report): Zinc chloride
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Age at study initiation: 2 months
- Weight at study initiation: 150-180 g
- Housing: Housed singly in cages
- Diet (e.g. ad libitum): Standard granulated rodent laboratory chow-LSM (CLPP, Motycz near Lublin)
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 18-20
- Humidity (%): 60±10
- Photoperiod (hrs dark / hrs light): Natural day-night light cycles
Administration / exposure
- Route of administration:
- oral: drinking water
- Vehicle:
- water
- Details on oral exposure:
- Not reported
- Analytical verification of doses or concentrations:
- no
- Details on analytical verification of doses or concentrations:
- Not applicable
- Duration of treatment / exposure:
- 28 d
- Frequency of treatment:
- Daily
Doses / concentrations
- Remarks:
- Doses / Concentrations:
0 and 0.12 mg Zn/cm3
Basis:
nominal in water
- No. of animals per sex per dose:
- Control group: 12 males and 12 females
Treatment group: 13 males and 17 females - Control animals:
- yes, concurrent vehicle
- Details on study design:
- Not reported
- Positive control:
- Not reported
Examinations
- Observations and examinations performed and frequency:
- BODY WEIGHT: Yes
FOOD CONSUMPTION AND COMPOUND INTAKE (if feeding study):
- Food consumption for each animal determined and mean daily diet consumption calculated as g food/kg body weight/day: Yes
HAEMATOLOGY: Yes
- Anaesthetic used for blood collection: Yes (ether)
- How many animals: 13 males and 17 females in treatment group and 12 males and 12 females in control group
- Parameters checked in table [No. 3, 4, 5 and fig. 2] were examined.
- Sacrifice and pathology:
- Not reported
- Other examinations:
- None
- Statistics:
- Students t-test at the significance level P < 0.05
Results and discussion
Results of examinations
- Clinical signs:
- effects observed, treatment-related
- Mortality:
- mortality observed, treatment-related
- Body weight and weight changes:
- no effects observed
- Food consumption and compound intake (if feeding study):
- effects observed, treatment-related
- Food efficiency:
- not examined
- Water consumption and compound intake (if drinking water study):
- not examined
- Ophthalmological findings:
- not examined
- Haematological findings:
- effects observed, treatment-related
- Clinical biochemistry findings:
- not examined
- Urinalysis findings:
- not examined
- Behaviour (functional findings):
- not examined
- Organ weight findings including organ / body weight ratios:
- not examined
- Gross pathological findings:
- not examined
- Histopathological findings: non-neoplastic:
- no effects observed
- Histopathological findings: neoplastic:
- not examined
- Details on results:
- CLINICAL SIGNS AND MORTALITY: No mortality observed (For details see table 1)
BODY WEIGHT AND WEIGHT GAIN: Body weight increment of treated animals was similar to that of the controls. (For details see table 1 and fig. 1)
FOOD CONSUMPTION AND COMPOUND INTAKE (if feeding study): Statistically significant decrease in food consumption was noted in treated animals as compared to that of the control group. (For detials see table 2)
HAEMATOLOGY: Statistically significant decrease in the erythrocyte count and haemoglobin level was found in the peripheral blood of treated animals. The haematocrit index decreased only slightly. (For details see table 3). Statistically significant increase in leukocyte count was noted in the treated males (For details see table 3). Percentage of reticulocytes and polychromatophilic erythrocytes in the peripheral blood of treated animals increased significantly as compared with that in the controls (For details see fig. 2). No major changes in the percentage composition of bone marrow cells between the control and treated animals observed. (For details see table 5)
Effect levels
- Dose descriptor:
- other: Significant increase in percentage of reticulocytes and polychromatophilic erythrocytes and statistically significant decrease in the erythrocyte count and haemoglobin level in the peripheral blood observed.
- Effect level:
- 0.12 other: mg Zn/cm3
- Sex:
- male/female
- Basis for effect level:
- other: Haematology
Target system / organ toxicity
- Critical effects observed:
- not specified
Any other information on results incl. tables
Table1: The effect of zinc chloride (ZC) on body weight gain in rats
Group of animals | Initial number of animals | Mortality | Number of animals examined | Body weight gain | |
Males | g | g/24 h | |||
Control | 12 | 0 | 12 | 84.83 ± 6.42 | 3.03 ± 0.23 |
ZC | 13 | 0 | 13 | 78.81 ± 4.07 | 2.82 ± 0.14 |
Females | |||||
Control | 12 | 0 | 12 | 36.70 ± 2.41 | 1.32 ± 0.09 |
ZC | 17 | 0 | 17 | 40.53 ± 2.49 | 1.45 ± 0.09 |
Table 2: Food, fluid and zinc intake in the studied animal groups
Group of animals | Food intake (g/rat/24 h) | Fluid intake (cm3/rat/24h) | Zinc (mg/kg body wt/24 h) |
Males | |||
Control | 27.02 ± 0.64 | 26.40 ± 0.70 | - |
ZC | 22.97 ± 0.40 | 19.85 ± 0.46*** | 11.66 ± 0.61 |
Females | |||
Control | 20.83 ± 0.34 | 25.20 ± 1.35 | - |
ZC | 19.79 ± 0.27* | 18.36 ± 0.85*** | 12.75 ± 1.09 |
Table 3: Haematological indices of peripheral blood in the studied animal groups
Group of animals | Erythrocytes x1012/dm3 | Haemoglobin nmol/L | Haematocrit (I)a | Leukocytes x 109/dm3 |
Males | ||||
Control | 8.25 ± 0.10 | 9.96 ± 0.22 | 0.48 ± 0.003 | 8.73 ± 0.72 |
ZC | 7.67 ± 0.15** | 8.79 ± 0.23** | 0.46 ± 0.004 | 12.60 ± 1.09* |
Females | ||||
Control | 8.31 ± 0.09 | 10.27 ± 0.34 | 0.48 ± 0.003 | 8.86 ± 0.65 |
ZC | 7.46 ± 0.15*** | 8.56 ± 0.24** | 0.46 ± 0.005 | 10.72 ± 0.94 |
a unit "one"
Table 4: Leukocyte picture of peripheral blood in rats
Group of animals | Several forms of leukocytes (109/dm3 | |||
Neutrophils | Eosinophils | Monocytes | Lymphocytes | |
Males | ||||
Control | 1.23 ± 0.20 | 0.13 ± 0.03 | 0.35 ± 0.06 | 7.02 ± 049 |
ZC | 2.40 ± 0.45* | 0.12 ± 0.02 | 0.35 ± 0.05 | 9.73 ± 0.73** |
Females | ||||
Control | 1.44 ± 0.21 | 0.17 ± 0.03 | 0.41 ± 0.06 | 6.85 ± 0.46 |
ZC | 1.59 ± 0.19 | 0.21 ± 0.05 | 0.36 ± 0.04 | 8.56 ± 0.72 |
Table 5: % composition of bone marrow cells in the studied animal groups
Males | Females | |||
Number of animals | Control | ZC | Control | ZC |
Proerythroblasts | 0.40±0.08 | 0.35 ± 0.06 | 0.44 ± 0.07 | 0.52±0.09 |
Basophilic erythroblasts | 2.98 ± 0.20 | 2.80 ± 0.02 | 3.46 ± 0.23 | 3.25 ± 0.24 |
Polychroamtophilic eryhtoroblasts | 9.81 ± 0.41 | 9.46 ± 0.35 | 10.36 ± 0.48 | 9.97 ± 0.41 |
Orthochromatic eryhtoroblasts | 23.08 ± 0.93 | 24.51 ± 1.13 | 24.21 ± 1.08 | 22.61 ± 0.71 |
Myeloblasts | 2.39 ± 0.19 | 1.76 ± 0.10* | 2.75 ± 0.30 | 2.30 ± 0.14 |
Neutrophil granulocytes | 29.89 ± 1.01 | 27.78 ± 1.43 | 25.78 ± 1.57 | 28.32 ± 0.84 |
Eosinophil granulocytes | 3.55 ± 0.32 | 2.95 ± 0.25 | 5.36 ± 0.75 | 4.09 ± 0.33 |
Basophil granulocytes | 0.79 ± 0.08 | 1.08 ± 0.14 | 0.92 ± 0.12 | 1.22 ± 0.18 |
Lymphocytes | 18.26 ± 1.06 | 20.37 ± 2.19 | 18.33 ±0.90 | 19.26 ± 0.88 |
Monocytes | 2.12 ± 0.13 | 2.34 ± 0.17 | 1.94 ± 0.12 | 2.09 ± 0.12 |
Plasma cells | 0.40 ± 0.09 | 0.41± 0.07 | 0.52 ± 0.09 | 0.36 ± 0.06 |
Reticulum cells | 5.72 ± 0.24 | 5.59 ± 0.23 | 5.26 ± 0.45 | 5.44 ± 0.31 |
Macrophagic reticulum cells | 0.53 ± 0.08 | 0.60 ± 0.08 | 0.68 ± 0.09 | 0.56 ± 0.06 |
Significantly different from control group: *P < 0.05, **P < 0.01, ***P < 0.001
Applicant's summary and conclusion
- Conclusions:
- Under the conditions of this test, the test material was observed to alter haematological parameters in Wistar rats.
- Executive summary:
A study was conducted in Wistar rats to assess the effect of test material on certain haematological parameters.
Animals were treated orally at a concentration of 0.12 mg Zn/cm3 daily for 28 d. Significant increase in percentage of recticulocytes and polychromatophilic erythrocytes and statistically significant decrease in the erythrocyte count and haemoglobin level in the peripheral blood was observed. No major changes in the percentage composition of bone marrow cells between the control and treated animals were observed.
Under the conditions of this test, the test material was observed to alter haematological parameters in Wistar rats.
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