8,9,10-trinorborn-2-ene

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: acceptable publication which meets basic scientic principles; basic data on expermimental design are given.

Data source

Reference

Reference 1

Reference Type:

publication

Title:

Range-Finding Toxicity Data: List VII

Author:

Smyth, HF; Carpenter, CP; Weil, CS; Pozzani UC; Striegel JA; Nycum, JS

Year:

1962

Bibliographic source:

Amer. Ind. Hyg. Assoc. J. 30 (1969), 470

Report date:

1969

Reference 2

Reference Type:

publication

Title:

Range-Finding Toxicity Data: List VI

Author:

Smyth, HF; Carpenter, CP; Weil, CS; Pozzani UC; Striegel JA

Year:

1962

Bibliographic source:

Amer. Ind. Hyg. Assoc. J. 23 (1962), 95

Report date:

1962

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 402 (Acute Dermal Toxicity)

Deviations:

yes

Remarks:

only 4 animal per group, occlusive wrapping, limited reporting

Principles of method if other than guideline:

pre-guidelien method similar to OECD 402

GLP compliance:

no

Remarks:

pre-GLP study

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

8,9,10-trinorborn-2-ene

EC Number:

207-866-0

EC Name:

8,9,10-trinorborn-2-ene

Cas Number:

498-66-8

Molecular formula:

C7H10

IUPAC Name:

Bicyclo-[2.2.1]-hept-2-ene

Details on test material:

no further details specified

Test animals

Species:

rabbit

Strain:

New Zealand White

Sex:

male

Details on test animals or test system and environmental conditions:

weight at study initiation: 2.5 -3.5 kg

Administration / exposure

Type of coverage:

occlusive

Vehicle:

unchanged (no vehicle)

Details on dermal exposure:

TEST SITE
- Area of exposure: part of the trunk
- % coverage: 1/10 of body surface
- Type of wrap if used: impervious plastic film

REMOVAL OF TEST SUBSTANCE
- Washing (if done): no data
- Time after start of exposure:24 h

TEST MATERIAL
- Amount(s) applied (volume or weight with unit): graduate doses were applied, but amount of applied substance was not specified

Duration of exposure:

24 h

Doses:

graduated doses, details not reported

No. of animals per sex per dose:

4, males only

Control animals:

not specified

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: no data
- Necropsy of survivors performed: no data
- Other examinations performed: clinical signs, body weight,organ weights, histopathology, other: no data

Statistics:

LD50 values calculated by method of THompson (1947, Bacteriol.Rev. 11,115) using the tables of Weil (1952, Biometrics 8, 249). confidence intervals of +/- 1.96 standard deviations are given.

Results and discussion

Effect levels

Sex:

male

Dose descriptor:

LD50

Effect level:

> 5 mL/kg bw

Remarks on result:

other: corresponds to 4353 mg/kg

Any other information on results incl. tables

Fiducial range of LD50 values is not given. Values converted to mg/kg are LD50 > 4353 mg/kg

Applicant's summary and conclusion

Interpretation of results:

not classified

Remarks:

Migrated information Criteria used for interpretation of results: EU

Conclusions:

the acute dermal toxicity was > 4353 mg/kg b.w. in male albino New Zealand rabbits.

Executive summary:

the acute dermal toxicity was determined in groups of 4 male albino New Zealand rabbits, each receiving a graduate single dose of test substance followed by 24h occlusive wrapping and 14 d observation. From mortality data the LD50 was calculated as > 5 ml/kg corresponding to > 4353 mg/kg.