Selenious acid

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Well performed study. Conducted according to US EPA guidelines for acute testing. LC50 values based on measured Se concentrations.

Data source

Reference

Reference Type:

publication

Title:

Comparative Toxicity of Selenate, Selenite, Seleno-DL-Methionine and Seleno-DL-Cystine to Daphnia Magna

Author:

Maier KJ, Foe CG, Knight AW

Year:

1993

Bibliographic source:

Environmental Toxicology and Chemistry 12: 755-763

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: EPA-660/3-75-009: Methods for Acute Toxicity Tests with Fish, Macroinvertebrates, and Amphibians

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Sodium selenite

EC Number:

233-267-9

EC Name:

Sodium selenite

Cas Number:

10102-18-8

Molecular formula:

H2O3Se.2Na

IUPAC Name:

disodium selenite

Details on test material:

- Name of test material (as cited in study report): Selenite
- Molecular formula (if other than submission substance): Na2SeO3
- Molecular weight (if other than submission substance): 173 g/mol
- Analytical purity: no data
- Source: Sigma Chemical Co., St. Louis, MO, USA

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Frequency: At the start and end of exposure

Test solutions

Vehicle:

no

Details on test solutions:

see test conditions

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Source: Laboratory stock
- Age at study initiation: Neonates
- Feeding during test: No

ACCLIMATION
- Acclimation period: no data

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

as measured ± 3% mg/L as CaCO3

Test temperature:

20 ± 0.1 °C

pH:

8.2 ± 0.1

Dissolved oxygen:

8.6 ± 0.2 mg/L

Salinity:

Not applicable

Nominal and measured concentrations:

as calculated ± 5% mg Se/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 500 ml acid-washed glass jars with 100 mL of test medium
- Renewal rate of test solution (frequency/flow rate): 95% of solution replaced at 24 hours
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 5
- No. of vessels per control (replicates): 5

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Reconstituted, moderately hard, fresh water that contained salt concentrations twice those in EPA soft fresh water. The initial water was reverse-osmosis-treated water that met EPA specifications, to which 96 mg/L NaHCO3, 60 mg/L CaSO4.2H2O, 60 mg/L MgSO4, and 4 mg/L KCl (all reagent grade) were added.
- Sulfate: 81.5 mg/L

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

550 µg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

element

Remarks:

Se

Basis for effect:

mortality

Reported statistics and error estimates:

LC50 values were interpolated by probit analysis, using the SAS statistical program.

Applicant's summary and conclusion

Conclusions:

Reliable (Klimisch 1) study on toxicity of sodium selenite to Daphnia magna. The 48-h LC50 value was 550 µg Se/L.