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Diss Factsheets

Administrative data

Endpoint:
acute toxicity: dermal
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Meets generally accepted scientific standards, well documented and acceptable for assessment

Data source

Reference
Reference Type:
publication
Title:
Range-Finding Toxicity Data: List IV
Author:
Smyth HF, Carpenter CP, Weil CS
Year:
1951
Bibliographic source:
Arch.Ind.Hyg.Occup.Med. 4: 119-122

Materials and methods

Principles of method if other than guideline:
Penetration of rabbit skin is estimated by a technique closely akin to the one-day, cuff Method of Draize and associates using groups of four male albino New Zealand rabbits weighing 2.5 to 3.5 kg. The fur is removed from the entire trunk by clipping, and the dose is retained beneath an impervious plastic film. Dosages greater than 20 ml/kg cannot be retained in contact with the skin. The animals are immobilized during the 24-hour contact period, after which the film is removed and the rabbits are caged for the subsequent 14-day observation period. Based upon mortalities during a 14-day observation period, the most probable LD50 value and its fiducial range are estimated by the method of Thompson using the Tables of Weil.
GLP compliance:
no
Test type:
standard acute method

Test material

Constituent 1
Chemical structure
Reference substance name:
Isoquinoline
EC Number:
204-341-8
EC Name:
Isoquinoline
Cas Number:
119-65-3
Molecular formula:
C9H7N
IUPAC Name:
isoquinoline
Details on test material:
- Name of test material (as cited in study report): Iso quinoline

Test animals

Species:
rabbit
Strain:
New Zealand White
Sex:
male
Details on test animals or test system and environmental conditions:
New Zealand rabbits weighing 2.5 to 3.5 kg

Administration / exposure

Type of coverage:
occlusive
Vehicle:
not specified
Details on dermal exposure:
The fur is removed from the entire trunk by clipping, and the dose is retained beneath an impervious plastic film. Dosages greater than 20 ml/kg cannot be retained in contact with the skin. The animals are immobilized during the 24-hour contact period, after which the film is removed and the rabbits are caged for the subsequent 14-day observation period.
Duration of exposure:
24 hours
Doses:
no data
No. of animals per sex per dose:
4 male animals per dose
Control animals:
not specified
Details on study design:
- Duration of observation period following administration: 14 days
no further data
Statistics:
Based upon mortalities during a 14-day observation period, the most probable LD50 value and its fiducial range are estimated by the method of Thompson using the Tables of Weil.

Results and discussion

Effect levels
Sex:
male
Dose descriptor:
LD50
Effect level:
648 mg/kg bw
Based on:
test mat.
95% CL:
> 417 - < 1 000
Remarks on result:
other: LD50 = 0.59 ml/kg according to the publication, the values were converted based on a density of 1.099 g/cm3
Mortality:
no data
Clinical signs:
no data
Body weight:
no data
Gross pathology:
no data
Other findings:
no data

Applicant's summary and conclusion