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Toxicological information

Genetic toxicity: in vitro

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Administrative data

Endpoint:
in vitro gene mutation study in bacteria
Remarks:
Type of genotoxicity: gene mutation
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Meets generally accepted scientific standards, well documented and acceptable for assessment

Data source

Reference
Reference Type:
publication
Title:
Mutagenicities of quinoline and its derivatives
Author:
Nagao M, Yahagi T, Seino Y, Sugimura T, Ito N
Year:
1977
Bibliographic source:
Mutation Research 42: 335-342

Materials and methods

Principles of method if other than guideline:
The mutagenicity of isoquinoline was tested in Salmonella typhimurium tester trains TA98 and TA100.
GLP compliance:
not specified
Type of assay:
bacterial reverse mutation assay

Test material

Constituent 1
Chemical structure
Reference substance name:
Isoquinoline
EC Number:
204-341-8
EC Name:
Isoquinoline
Cas Number:
119-65-3
Molecular formula:
C9H7N
IUPAC Name:
isoquinoline
Details on test material:
- Name of test material (as cited in study report): isoquinoline
- Analytical purity: purest grade available

Method

Target gene:
his gene
Species / strainopen allclose all
Species / strain / cell type:
S. typhimurium TA 98
Additional strain / cell type characteristics:
not applicable
Species / strain / cell type:
S. typhimurium TA 100
Additional strain / cell type characteristics:
not applicable
Metabolic activation:
with and without
Metabolic activation system:
S-9 mix
Test concentrations with justification for top dose:
1-20 umol/plate
Vehicle / solvent:
- Vehicle(s)/solvent(s) used: [no data]
Controls
Untreated negative controls:
not specified
Negative solvent / vehicle controls:
not specified
True negative controls:
not specified
Positive controls:
not specified
Positive control substance:
not specified
Details on test system and experimental conditions:
Assay was carried out as described by Ames et al. with some modifications. Chemicals freshly dissolved in 100 ul of DMSO were pre-incubated at 37°C for 20 min with 0.5 ml of S-9 mix or 0.5 ml of 0.1 M sodium phosphate buffer (pH 7.4) and 0.1 ml of bacterial culture. Two ml of molten soft agar at 45°C were added, and the resulting mixture was poured over 25 ml of minimal-glucose agar containing 0.1 umol of L-histidine and 0.1 umol of biotin. After 2-day incubation, colonies of histidine prototroph were counted as revertants.
Evaluation criteria:
no data
Statistics:
no data

Results and discussion

Test resultsopen allclose all
Species / strain:
S. typhimurium TA 98
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
cytotoxicity
Remarks:
7 umol/plate and up (without S-9 mix), 10 umol/plate with S-9 mix
Vehicle controls validity:
not specified
Untreated negative controls validity:
not specified
Positive controls validity:
not specified
Species / strain:
S. typhimurium TA 100
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
cytotoxicity
Remarks:
7 umol/plate and up (without S-9 mix), 10 umol/plate with S-9 mix
Vehicle controls validity:
not specified
Untreated negative controls validity:
not specified
Positive controls validity:
not specified
Remarks on result:
other: all strains/cell types tested
Remarks:
Migrated from field 'Test system'.

Applicant's summary and conclusion

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