Registration Dossier
Registration Dossier
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EC number: 204-341-8 | CAS number: 119-65-3
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Meets generally accepted scientific standards, well documented and acceptable for assessment
Data source
Reference
- Reference Type:
- publication
- Title:
- Mutagenicities of quinoline and its derivatives
- Author:
- Nagao M, Yahagi T, Seino Y, Sugimura T, Ito N
- Year:
- 1 977
- Bibliographic source:
- Mutation Research 42: 335-342
Materials and methods
- Principles of method if other than guideline:
- The mutagenicity of isoquinoline was tested in Salmonella typhimurium tester trains TA98 and TA100.
- GLP compliance:
- not specified
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Isoquinoline
- EC Number:
- 204-341-8
- EC Name:
- Isoquinoline
- Cas Number:
- 119-65-3
- Molecular formula:
- C9H7N
- IUPAC Name:
- isoquinoline
- Details on test material:
- - Name of test material (as cited in study report): isoquinoline
- Analytical purity: purest grade available
Constituent 1
Method
- Target gene:
- his gene
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 98
- Additional strain / cell type characteristics:
- not applicable
- Species / strain / cell type:
- S. typhimurium TA 100
- Additional strain / cell type characteristics:
- not applicable
- Metabolic activation:
- with and without
- Metabolic activation system:
- S-9 mix
- Test concentrations with justification for top dose:
- 1-20 umol/plate
- Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: [no data]
Controls
- Untreated negative controls:
- not specified
- Negative solvent / vehicle controls:
- not specified
- True negative controls:
- not specified
- Positive controls:
- not specified
- Positive control substance:
- not specified
- Details on test system and experimental conditions:
- Assay was carried out as described by Ames et al. with some modifications. Chemicals freshly dissolved in 100 ul of DMSO were pre-incubated at 37°C for 20 min with 0.5 ml of S-9 mix or 0.5 ml of 0.1 M sodium phosphate buffer (pH 7.4) and 0.1 ml of bacterial culture. Two ml of molten soft agar at 45°C were added, and the resulting mixture was poured over 25 ml of minimal-glucose agar containing 0.1 umol of L-histidine and 0.1 umol of biotin. After 2-day incubation, colonies of histidine prototroph were counted as revertants.
- Evaluation criteria:
- no data
- Statistics:
- no data
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- 7 umol/plate and up (without S-9 mix), 10 umol/plate with S-9 mix
- Vehicle controls validity:
- not specified
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- not specified
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- 7 umol/plate and up (without S-9 mix), 10 umol/plate with S-9 mix
- Vehicle controls validity:
- not specified
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- not specified
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
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Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
