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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
acute toxicity: inhalation
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1964-12-02 - 1965-01-1
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1965
Report date:
1965

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 403 (Acute Inhalation Toxicity)
Deviations:
not specified
GLP compliance:
no
Test type:
standard acute method
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
3,3,5-trimethylcyclohexan-1-one
EC Number:
212-855-9
EC Name:
3,3,5-trimethylcyclohexan-1-one
Cas Number:
873-94-9
Molecular formula:
C9H16O
IUPAC Name:
3,3,5-trimethylcyclohexan-1-one
Details on test material:
Test substance: other TS: Origin: Esso Research and Engineering Company, 2 Dec 1964,

Test animals

Species:
rat
Strain:
Wistar
Sex:
male
Details on test animals or test system and environmental conditions:
TEST ORGANISMS: 
- Weight at study initiation: 200-225 grams
- Number of animals: 10
- Controls: yes

Administration / exposure

Route of administration:
inhalation: aerosol
Type of inhalation exposure:
whole body
Vehicle:
air
Details on inhalation exposure:
- Type of exposure: inhalation, aerosol (mist)
- The test atmosphere is not well characterized. However, due to a saturation vapour pressure of 3.45 mg/l and the applied technique (use of precision metering pump into a stainless steel spray nozzle tip) it can be concluded that the atmosphere was a mist rather than a vapour.
Analytical verification of test atmosphere concentrations:
not specified
Duration of exposure:
4 h
Concentrations:
- Concentrations: 11.5; 15; 18 mg/l
No. of animals per sex per dose:
10 males
Control animals:
yes
Details on study design:
EXAMINATIONS: 
- Observations for deaths and toxic signs: 30 min intervals
- post observation: 14 days, daily
- gross pathology: at end of post observation period: all surviving animals
- vaporization of the material was achieved by means of a precision metering pump into a stainless steel spray nozzle tip.
Statistics:
no data

Results and discussion

Effect levels
Sex:
male
Dose descriptor:
LC50
Effect level:
14.2 mg/L air (nominal)
Exp. duration:
4 h
Remarks on result:
other: aerosol
Mortality:
- Number of deaths at each dose, time of death:     
11.5 mg/L: 4 hours = 0; 14 days = 0 death     
15.0 mg/L: 4 hours = 0; 14 days = 8 deaths
18.0 mg/L: 4 hours = 10 deaths
LD50 confidence intervals: 12.6 - 16.0 mg/L; slope function 1.18
Clinical signs:
other: During exposure, the animals generally exhibited hyperactivity and towards the end of the exposure became prostrate and comatose. Following exposure, the anomals showed depression and decrease of activity, piloerection and weight loss.
Body weight:
weight loss
Gross pathology:
Autopsy of the high dose animals revealed moderate atelectasis of the lung and congestion of the kidneys, findings in surviving animals were not significant.
Other findings:
no other findings

Any other information on results incl. tables

no remarks

Applicant's summary and conclusion

Conclusions:
Under the conditions of this study, the LC50 of 3,3,5-trimethylcyclohexanone after exposure by inhalation was determined to be 14.2 mg/l in male rats.
Executive summary:

Groups of 10 male rats were exposed to 3,3,5-trimethylcyclohexanone for 4 hours by whole body exposure. The observation period was 2 weeks. At a concentration of 11.5 mg/l no animal died. At a concentration of 15.0 mg/l 80 % of the rats died after the observation period of 14 days. At very high concentrations of 18.0 mg/l all animals died within 4 hours. Clinical signs were depression and decrease of activity, piloerection and weight loss. At necropsy of the high exposure concentrations, moderate atelectasis of the lung and congestion of the kidneys were notified, surviving animals were not significant

Therefore, under the conditions of this study the LC50 of 3,3,5 -trimethylcyclohexanone after inhalative exposure in rats was 14.2 mg/l.