2,5-dimethoxyaniline

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1989

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Study performed to OECD 203 including GLP certificate and well documented

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1989

Report date:

1989

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

other: "Letale Wirkung beim Zebrabärbling Brachydanio rerio" (LC0, LC50, LC100; 48 - 96 Stunden) - recommendation of the GErman Federal Environmetal Agency, Texte 16/84, Umweltbundesamt

Deviations:

no

Test guideline 2

Qualifier:

equivalent or similar to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Deviations:

not specified

Test guideline 3

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

not specified

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

2,5-dimethoxyaniline

EC Number:

203-040-9

EC Name:

2,5-dimethoxyaniline

Cas Number:

102-56-7

Molecular formula:

C8H11NO2

IUPAC Name:

2,5-dimethoxyaniline

Details on test material:

molecular formula C8-H11-N-O2
molecular weight 153,5 g/mol

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

Actual substance concentration were determined at the beginning, after 48 h and at the end of the experiment at 96 h. Therefore 100 ml of the solution was taken out of the bath middle. The analytical data were given above 80 % of target concentration.
Target (mg/l) meassured median (mg/l) measured (%)
100 100.1 100.1
220 220.0 100.0
500 562.0* 112,4
* median after 0 and 48 hours

Test solutions

Vehicle:

yes

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

Strain: Hamilton-Buchanan
Origin West Aquarium, 3422 Bad Lauterberg, Germany
Date of hatching: 1989-01-12 / 1989-01-19
Date of transfer to test basins: 1989-04-12 / 1989-04-19

Acclimation period of 14 days in dilution water (test water according to ISO/DIS 7346/1):
Temperature 22 +/- 1 °C
O2-content >= 80 % of Saturation
Illumination 12 h daily
Biomasse loading: <= 1 g Fish / l water
Feeding: 2 times a day ad libitum
Food: Tetra Min, Tetra Werke, Melle, Germany

The experiment was perfomred in three parts: part 1 0, 0* and 100 mg/lwith 0* being the vehicle control with Tween 80), part 2 (0 and 500 mg/l), part 3 (0 and 220 mg/l). From the fish for each test-part 10 have been choosen for determination of body length. Those enimals were not used for the test
part 1: n=10, range = 2.6 - 3.3 cm, mean = 2,9 cm, SD = 0,20 cm
part 2: n=10, range = 2.8 - 3.5 cm, mean = 3.1 cm, SD = 0,26 cm
part 1: n=10, range = 2.9 - 3.4 cm, mean = 3.1 cm, SD = 0,19 cm
total: n=30, range = 2.6 - 3.5 cm, mean = 3.1 cm, SD = 0,24 cm

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Post exposure observation period:

none

Test conditions

Hardness:

test water (reconstituted fresh water) according to ISO/DIS 7346/1

Test temperature:

22 +/- 1 °C

pH:

concentration groups 7.3 - 8.1
controls 7.5 - 8.2

Dissolved oxygen:

concentration groups 4.6 - 8.6 mg/l
controls 4.2 - 9.1 mg/l

Salinity:

test water (reconstituted fresh water) according to ISO/DIS 7346/1

Nominal and measured concentrations:

At the start, after 48 h and at the end of the test (96 h) the actual concentration for all the dilutions were determined (100 ml samples each). The arithmetic means fo the determined concentrations were (mg/l):

nominal analytically measured
100 100,1
220 220,0
500 562,0

Details on test conditions:

Test system:
Test vessel: Glass basins, volume 10 l (30 cm * 22 cm * 24 cm)
Aeration: For concentration group 220 mg/l of part 3 and the Tween group from 72 h after start of the experiment aeratio had been applied to acertain a minimum O2-saturation from 60 % saturation. For all other groups saturation was not neccessary.
No of organism per vessel: 10
No of vessels per concentration: one
No of vessels per control (replicas): three
No of vessels per vehicle control: one

Photoperiod: 12 h, approx 700 LUX
Adjustment of pH: no

Reference substance (positive control):

yes

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

LC0

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 3

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

> 220 - < 500 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 4

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 220 - < 500 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 5

Duration:

48 h

Dose descriptor:

LC100

Effect conc.:

500 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 6

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

500 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

In an acute toxicity test on Brachydanio rerio equivalent to OECD 203, the substance Aminohydrochinondimethylether was tested for acute toxicity. The LC0 was 100 mg/l after both, 48 and 96 hours. The LC50 was determined to be between 220 and 500 mg/l, after 48 and 96 hours.

Executive summary:

In an akute toxicity test with Brachydanio rerio equivalent to OECD 203, the substance Aminohydrochinondimethylether was tested for acute toxicity. The LC0 was 100 mg/l after both, 48 and 96 hours. The LC50 was determined to be between 220 and 500 mg/l, after 48 and 96 hours.