1,3,5-triazine-2,4,6(1H,3H,5H)-trithione, trisodium salt

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1993

Report date:

1993

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 402 (Acute Dermal Toxicity)

Deviations:

no

GLP compliance:

yes

Test type:

standard acute method

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

1,3,5-triazine-2,4,6(1H,3H,5H)-trithione, trisodium salt

EC Number:

241-749-5

EC Name:

1,3,5-triazine-2,4,6(1H,3H,5H)-trithione, trisodium salt

Cas Number:

17766-26-6

Molecular formula:

C3N3S3.3Na

IUPAC Name:

trisodium trisulfanylidene-1,3,5-triazinane-1,3,5-triide

Test animals

Species:

rat

Strain:

other: CD(SD)BR VAF/PlusT

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Age at study initiation: males 7 weeks, females 8 weeks
- Weight at study initiation: males 200-253 g, females 197-222 g
- Housing: individually in stainless-steel mesh cages
- Diet: Agway Prolab Animal diet (RMH 3000) certified pellets, ad libitum
- Water: ad libitum
- Acclimation period: 5 days

ENVIRONMENTAL CONDITIONS
- Temperature (° C): 21-22
- Humidity (%): 59-66
- Photoperiod (hrs dark / hrs light): 12/12

IN-LIFE DATES: From: 04 May 1993 To: 09 June 1993

Administration / exposure

Type of coverage:

occlusive

Vehicle:

unchanged (no vehicle)

Details on dermal exposure:

TEST SITE
- Area of exposure: an area of the dorsal skin
- Type of wrap if used: a fiber pad and an occlusive wrap

REMOVAL OF TEST SUBSTANCE
- Washing (if done): running water
- Time after start of exposure: 24 h

TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 2000 mg/kg bw
- Constant volume or concentration used: yes
- For solids, paste formed: the test material was administered as a solid moistened thoroughly with water

Duration of exposure:

24 hours

Doses:

2000 mg TMT 55/kg bw (equivalent to 1100 mg anhydrous TMT/kg bw)

No. of animals per sex per dose:

5

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations (mortality, clinical signs, irritation using Draize scale): at day 0 and once daily thereafter (a total of 14 calendar days)
- Frequency of weighing: day 0 (prior to dosing), day 7 and day 14
- Necropsy of survivors performed: all animals were necropsied at the completion of the 14-day observation period

Results and discussion

Effect levels

Effect levels 1

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 2 000 mg/kg bw

Based on:

test mat.

Remarks on result:

other: hydrated form TMT 55

Effect levels 2

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 1 100 mg/kg bw

Based on:

other: anhydrous form

Remarks on result:

other: recalculated value for anhydrous TMT based on 55% TMT

Mortality:

All animals survived to the end of the study.

Clinical signs:

other: Skin irritation was observed ranging from barely perceptible signs of irritation (slight erythema), to signs of strong irritation (servere erythema, slight edema) with irreversible skin damage. At the end of the observation period, scars were present at t

Gross pathology:

A large spleen, noted for a single male, was processed for microscopic examination. Histologically, the increased size of the spleen was associated with hyperplastic lymphoid follicles. The cause for this change was not identified, but may have been related to the inflammatory response associated with the skin damage seen in this rat. No other treatment-related changes or signs of organ toxicity were evident at necropsy.

Applicant's summary and conclusion

Interpretation of results:

other: not classified for hydrated form TMT 55; inconclusive for anhydrous TMT

Remarks:

Criteria used for interpretation of results: OECD GHS

Conclusions:

In this acute dermal toxicity study in the rat the LD50 for the test item TMT 55 was greater than 2000 mg/kg bw (corresponding to > 1100 mg anhydrous TMT/kg bw) for both sexes.