Diisopropylbiphenyl and triisopropylbiphenyl

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Jan 21, 1986 - May 16, 1986

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1986

Report date:

1986

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

other: FIFRA (US EPA)

Test guideline 2

Qualifier:

according to guideline

Guideline:

other: TSCA (US EPA)

GLP compliance:

not specified

Test type:

standard acute method

Test material

Test material information

Constituent 1

Reference substance name:

Diisopropylbiphenyl and triisopropylbiphenyl

EC Number:

915-589-8

Molecular formula:

not applicable for UVCB substance

IUPAC Name:

Diisopropylbiphenyl and triisopropylbiphenyl

Details on test material:

- Name of test material (as cited in study report): Diisopropyl biphenyl, isomeric mixture (acronym used: DIPB or di-IPB/tri-IPB)
- Substance type: organic
- Density: 0.9669 g/ml
- Physical state: clear liquid
- Analytical purity: no data
- Impurities (identity and concentrations): no data
- Isomers composition: no data
- Lot/batch No.: NBP-3184823
- Expiration date of the lot/batch: no data
- Storage condition of test material: room temperature

Test animals

Species:

rat

Strain:

other: CDR (Sprague-Dawley derived)

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Species: Albino rats, CDR (Srague-Dawley derived)
- Source: Charles River Breeding Laboratories, Inc; Wilmington , Massachusetts, USA
- Age at study initiation: Young adults /approx. 9-12 weeks
- Weight at study initiation (prefasted): - Males: 250 - 338 g
- Females: 212 - 269 g
- Fasting period before study: approx. 18 h
- Housing: Group housed (6 per cage) during acclimation, individually during study in suspended stainless steel cages with wire mesh bottoms
- Diet: Purina Laboratory Chow, ad libitum
- Water: ad libitum
- Acclimation period: 8, 10 or 21 days

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 19.5 - 24.5°C (= 67 - 76°F)
- Humidity (%): 30 - 70
- Air changes (per hr): no data
- Photoperiod (hrs dark / hrs light): 12/12

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

unchanged (no vehicle)

Details on oral exposure:

Test material was administered by oral intubation, using a ball-tipped intubation needle fitted onto a syringe

Doses:

4000 mg/kg, 5000 mg/kg, 6250 mg/kg

No. of animals per sex per dose:

5 (except 5000 mg/kg dose: 10 male, 5 female)

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations: 1 h, 2 h, and 4 h after application; thereafter daily for 14 days
- Frequency of weighing: Pre-fast, post-fast, day 7 and day 14
- Necropsy of survivors performed: yes
- Gross postmortem examinations were performed on all animals which died or were found dead during the study

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 5 000 mg/kg bw

Based on:

test mat.

Mortality:

see table below

Clinical signs:

other: No abnormalities were observed during 4 h after application. 24 h after application and/or day 2 most animals exhibited hypoactivity, soft stool, fecal and/or urinary staining. All surviving animals were free of significant abnormalities at termination of

Gross pathology:

Postmortem examinations of animals found dead during the study revealed primarily changes in lungs and gastrointestinal tract.
Several animals exhibited changes in the stomach and intestine which were suggestive of a corrosive effect.
Other changes appeared to represent autolytic changes or antemortem stress.

Any other information on results incl. tables

Dose levels and mortality (excerpt from original report):

Dose level (mg/kg)		Mortality
	Male	Female	Total	Time of death (day)
4000	1/5	1/5	2/10	2 - 3
5000 (A)	(4/5) a	1/5	a	2 – 7
5000 (B)	2/5	-	2/5	2 – 4
6250	2/5	1/5	3/10	2 - 3

a: Because the weight of males dosed initially at 5000 mg/kg [designated 5000 (A)] exceeded the weight range of males dosed at the other two levels, an additional group of males in the same weight range as males in the other two dose levels was dosed [(designated 5000 (B)].

Only data from the 5000 (B) group were considered for estimation of LD50.

Applicant's summary and conclusion

Interpretation of results:

not classified

Remarks:

Migrated information

Conclusions:

The test substance, alkylation and transalkylation products of biphenyl with propene, showed low toxicity only.