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EC number: 218-780-8 | CAS number: 2234-16-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Toxicity to microorganisms
Administrative data
- Endpoint:
- toxicity to microorganisms
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 3 (not reliable)
- Rationale for reliability incl. deficiencies:
- other: No guideline was followed. No data on GLP.
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 2 001
Materials and methods
- Principles of method if other than guideline:
- The 2-d ciliate (T. pyriformis) population growth impairment (IGC50) data was determined experimentally by a static 40-h assay. Stock solutions were prepared in dimethyl sulfoxide. Ciliate toxicological assessments were performed following the protocol described by Schultz 1977. The population density was measured spectrophotometrically at 540 nm. Test conditions allow for 8 to 10 cell cycles in control cultures. Test substance was tested in a range finder prior to testing in duplicate for three additional replicates. Two controls, one without test chemical but inoculated with T. pyriformis and the other, a blank with neither test chemical nor ciliates, were used to provide a measure of the test acceptability and as a basis for interpretation of treatment data. Each definitive test replicate consisted of six to eight different concentrations with duplicate flasks of each concentration. Only replicates with control-absorbency values between 0.60 and 0.90 were used in the analyses. The 50% growth inhibition concentration (IGC50) was determined using the probit analysis routine in the Statistical Analysis System (SASt) software. All statistical analyses were performed on nominal concentrations; chemical analyses of concentrations were not performed.
- GLP compliance:
- not specified
Test material
- Reference substance name:
- 1-(2,4-dichlorophenyl)ethan-1-one
- EC Number:
- 218-780-8
- EC Name:
- 1-(2,4-dichlorophenyl)ethan-1-one
- Cas Number:
- 2234-16-4
- Molecular formula:
- C8H6Cl2O
- IUPAC Name:
- 1-(2,4-dichlorophenyl)ethan-1-one
- Details on test material:
- - Name of test material (as cited in study report): 2,4-Dichloroacetophenone
- Purity: 95% or greater and was not repurified prior to use.
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- - Chemical name of vehicle (organic solvent, emulsifier or dispersant): dimethyl sulfoxide.
Test organisms
- Test organisms (species):
- Tetrahymena pyriformis
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 40 h
Test conditions
- Nominal and measured concentrations:
- Six to eight different concentrations with duplicate flasks of each concentration.
No details reported. - Details on test conditions:
- TEST CONCENTRATIONS
- Range finding study: The test substance was tested in a range finder prior to testing in duplicate for three additional replicates. Two controls, one without test chemical but inoculated with T. pyriformis and the other, a blank with neither test chemical nor ciliates, were used to provide a measure of the test acceptability and as a basis for interpretation of treatment data. Each definitive test replicate consisted of six to eight different concentrations with duplicate flasks of each concentration.
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Population density, spectrophotometrically measured at 540 mm. - Reference substance (positive control):
- not specified
Results and discussion
Effect concentrations
- Key result
- Duration:
- 40 h
- Dose descriptor:
- other: log (1/inhibitory growth concentration [IGC50])
- Effect conc.:
- 0.62 mmol/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Reported statistics and error estimates:
- The 50% growth inhibition concentration (IGC50) was determined using the probit analysis routine in the Statistical Analysis System (SASt) software.
Any other information on results incl. tables
Log (1/LC50) = 0.62 mmol/L
LC50 = 0.239 mmol/L
LC50 = 45.18 mg/L
Applicant's summary and conclusion
- Validity criteria fulfilled:
- not specified
- Conclusions:
- The log (1/inhibitory growth concentration [IGC50]) for 40h exposure of the test substance was reported to be 0.62 mmol/L (45.18 mg/L) in Tetrahymena pyriformis.
- Executive summary:
The 2-d ciliate (Tetrahymena pyriformis) population growth impairment (IGC50) data was determined experimentally by a static 40-h assay. Stock solutions were prepared in dimethyl sulfoxide. Ciliate toxicological assessments were performed following the protocol described by Schultz 1977. The population density was measured spectrophotometrically at 540 nm. Test conditions allow for 8 to 10 cell cycles in control cultures. Test substance was tested in a range finder prior to testing in duplicate for three additional replicates. Two controls, one without test chemical but inoculated with T. pyriformis and the other, a blank with neither test chemical nor ciliates, were used to provide a measure of the test acceptability and as a basis for interpretation of treatment data. Each definitive test replicate consisted of six to eight different concentrations with duplicate flasks of each concentration. Only replicates with control-absorbency values between 0.60 and 0.90 were used in the analyses. The 50% growth inhibition concentration (IGC50) was determined using the probit analysis routine in the Statistical Analysis System (SASt) software. All statistical analyses were performed on nominal concentrations; chemical analyses of concentrations were not performed. The log (1/inhibitory growth concentration [IGC50]) for 40h exposure of the test substance was reported to be 0.62 mmol/L (~45.18 mg/L) in Tetrahymena pyriformis.
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