Registration Dossier
Registration Dossier
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EC number: 279-505-5 | CAS number: 80584-91-4
Toxicity to terrestrial plants
Administrative data
- Endpoint:
- toxicity to terrestrial plants: short-term (with study design considered suitable for long-term assessment)
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1989-12-13 to 1990-02-16
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: GLP study. Deviations from the updated OECD-guideline (adopted 2006): Percent particles < 20 µm not determined. Photoperiod 16h light/ 8h dark not maintainet from day 4 to day 14 after begin exposition.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 990
- Report date:
- 1990
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 208 (Terrestrial Plants Test: Seedling Emergence and Seedling Growth Test)
- Deviations:
- yes
- Remarks:
- Percent particles < 20 micrometer not determined
- GLP compliance:
- yes
Test material
- Reference substance name:
- 6,6',6''-(1,3,5-triazine-2,4,6-triyltriimino)trihexanoic acid
- EC Number:
- 279-505-5
- EC Name:
- 6,6',6''-(1,3,5-triazine-2,4,6-triyltriimino)trihexanoic acid
- Cas Number:
- 80584-91-4
- Molecular formula:
- C21H36N6O6
- IUPAC Name:
- 6,6',6''-(1,3,5-triazine-2,4,6-triyltriimino)trihexanoic acid
- Test material form:
- solid: particulate/powder
Constituent 1
Test substrate
- Details on preparation and application of test substrate:
- - Method of mixing into soil (if used): In order to achieve the required test concentrations the dry premixes of test substance and sand were blended with the dry soil in a HOBART-blender. After dispensing this blend into the test containers the seeds were planted and covere with a thin layer of soil. The water was added subsequently
- Controls: Blank: Soil
Test organisms
open allclose all
- Species:
- Vicia sativa
- Details on test organisms:
- - Seed weight: 50.13 mg
- Seed viability: 94 %
- Species:
- Brassica rapa
- Details on test organisms:
- - Seed weight: 2.19 mg
- Seed viability: 98 %
- Species:
- Lolium perenne
- Details on test organisms:
- - Seed weight: 2.21 mg
- Seed viability: 94 %
Study design
- Test type:
- seedling emergence toxicity test
- Study type:
- laboratory study
- Substrate type:
- artificial soil
- Limit test:
- no
- Total exposure duration:
- 18 d
Test conditions
- Test temperature:
- 18 - 21°C
- pH:
- 7.2
- Details on test conditions:
- TEST SYSTEM
- Testing facility: growth chamber
- No. of replicates per treatment group: 4 replicates with 5 seeds each
- No. of replicates per control: 4 replicates with 5 seeds each
SOURCE AND PROPERTIES OF SUBSTRATE
- Composition (if artificial substrate): 80% "Loess" and 20% fine quartz sand
- Organic carbon (%): 0.23 %
GROWTH CONDITIONS
- Photoperiod: Photoperiod 16h light/ 8h dark not maintained from day 4 to day 14 after begin exposition (continous light during this period).
- Light intensity and quality: 2600 - 5000 Lux (52 - 100 µE/m2sec.)
- Air humidity (%): 56 - 83 %
- Watering regime and schedules: Daily control of weight (pot including soil, water, plant) from monday to friday and additon of water where necessary. - Nominal and measured concentrations:
- 1, 10, 100 mg/kg (nominal)
Results and discussion
Effect concentrationsopen allclose all
- Species:
- Lolium perenne
- Duration:
- 4 d
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- seedling emergence
- Species:
- Brassica rapa
- Duration:
- 3 d
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/kg soil ww
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- seedling emergence
- Species:
- Vicia sativa
- Duration:
- 4 d
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- seedling emergence
- Species:
- Lolium perenne
- Duration:
- 16 d
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth
- Species:
- Brassica rapa
- Duration:
- 16 d
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth
- Species:
- Vicia sativa
- Duration:
- 16 d
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth
- Details on results:
- Emergence:
EC 0 for all three species tested: 100 mg/kg
EC 100 for all three species tested: 100 mg/kg
Growth:
EC 0 (15-16d) in the test for LOLIUM PERENNE: 100 mg/kg
EC 0 (15-16d) in the test for BRASSICA RAPA: 100 mg/kg
EC 0 (15-16d) in the test for VICIA SATIVA: 100 mg/kg
EC 100 (15-16d) in the test for LOLIUM PERENNE: 100 mg/kg
EC 100 (15-16d) in the test for BRASSICA RAPA: 100 mg/kg
EC 100 (15-16d) in the test for VICIA SATIVA: 100 mg/kg
No visible phytotoxic effects were observed on all three species.
Applicant's summary and conclusion
- Executive summary:
Potential effects of the test item on seedling emergence and growth were assessed with OECD 208.
Emergence: EC 50 for all three species tested (LOLIUM PERENNE, BRASSICA RAPA, VICIA SATIVA) exceeded 100 mg/kg.
Growth: EC 50 for all three species tested (LOLIUM PERENNE, BRASSICA RAPA, VICIA SATIVA) exceeded 100 mg/kg.
No visible phytotoxic effects were observed on all three species.
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