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Administrative data

Endpoint:
toxicity to terrestrial plants: short-term (with study design considered suitable for long-term assessment)
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1989-12-13 to 1990-02-16
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: GLP study. Deviations from the updated OECD-guideline (adopted 2006): Percent particles < 20 µm not determined. Photoperiod 16h light/ 8h dark not maintainet from day 4 to day 14 after begin exposition.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1990
Report date:
1990

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 208 (Terrestrial Plants Test: Seedling Emergence and Seedling Growth Test)
Deviations:
yes
Remarks:
Percent particles < 20 micrometer not determined
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
6,6',6''-(1,3,5-triazine-2,4,6-triyltriimino)trihexanoic acid
EC Number:
279-505-5
EC Name:
6,6',6''-(1,3,5-triazine-2,4,6-triyltriimino)trihexanoic acid
Cas Number:
80584-91-4
Molecular formula:
C21H36N6O6
IUPAC Name:
6,6',6''-(1,3,5-triazine-2,4,6-triyltriimino)trihexanoic acid
Test material form:
solid: particulate/powder

Test substrate

Details on preparation and application of test substrate:
- Method of mixing into soil (if used): In order to achieve the required test concentrations the dry premixes of test substance and sand were blended with the dry soil in a HOBART-blender. After dispensing this blend into the test containers the seeds were planted and covere with a thin layer of soil. The water was added subsequently
- Controls: Blank: Soil

Test organisms

open allclose all
Species:
Vicia sativa
Details on test organisms:
- Seed weight: 50.13 mg
- Seed viability: 94 %
Species:
Brassica rapa
Details on test organisms:
- Seed weight: 2.19 mg
- Seed viability: 98 %
Species:
Lolium perenne
Details on test organisms:
- Seed weight: 2.21 mg
- Seed viability: 94 %

Study design

Test type:
seedling emergence toxicity test
Study type:
laboratory study
Substrate type:
artificial soil
Limit test:
no
Total exposure duration:
18 d

Test conditions

Test temperature:
18 - 21°C
pH:
7.2
Details on test conditions:
TEST SYSTEM
- Testing facility: growth chamber
- No. of replicates per treatment group: 4 replicates with 5 seeds each
- No. of replicates per control: 4 replicates with 5 seeds each

SOURCE AND PROPERTIES OF SUBSTRATE
- Composition (if artificial substrate): 80% "Loess" and 20% fine quartz sand
- Organic carbon (%): 0.23 %

GROWTH CONDITIONS
- Photoperiod: Photoperiod 16h light/ 8h dark not maintained from day 4 to day 14 after begin exposition (continous light during this period).
- Light intensity and quality: 2600 - 5000 Lux (52 - 100 µE/m2sec.)
- Air humidity (%): 56 - 83 %
- Watering regime and schedules: Daily control of weight (pot including soil, water, plant) from monday to friday and additon of water where necessary.


Nominal and measured concentrations:
1, 10, 100 mg/kg (nominal)

Results and discussion

Effect concentrationsopen allclose all
Species:
Lolium perenne
Duration:
4 d
Dose descriptor:
EC50
Effect conc.:
> 100 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
seedling emergence
Species:
Brassica rapa
Duration:
3 d
Dose descriptor:
EC50
Effect conc.:
> 100 mg/kg soil ww
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
seedling emergence
Species:
Vicia sativa
Duration:
4 d
Dose descriptor:
EC50
Effect conc.:
> 100 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
seedling emergence
Species:
Lolium perenne
Duration:
16 d
Dose descriptor:
EC50
Effect conc.:
> 100 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth
Species:
Brassica rapa
Duration:
16 d
Dose descriptor:
EC50
Effect conc.:
> 100 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth
Species:
Vicia sativa
Duration:
16 d
Dose descriptor:
EC50
Effect conc.:
> 100 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth
Details on results:
Emergence:
EC 0 for all three species tested: 100 mg/kg
EC 100 for all three species tested: 100 mg/kg
Growth:
EC 0 (15-16d) in the test for LOLIUM PERENNE: 100 mg/kg
EC 0 (15-16d) in the test for BRASSICA RAPA: 100 mg/kg
EC 0 (15-16d) in the test for VICIA SATIVA: 100 mg/kg
EC 100 (15-16d) in the test for LOLIUM PERENNE: 100 mg/kg
EC 100 (15-16d) in the test for BRASSICA RAPA: 100 mg/kg
EC 100 (15-16d) in the test for VICIA SATIVA: 100 mg/kg

No visible phytotoxic effects were observed on all three species.

Applicant's summary and conclusion

Executive summary:

Potential effects of the test item on seedling emergence and growth were assessed with OECD 208.

Emergence: EC 50 for all three species tested (LOLIUM PERENNE, BRASSICA RAPA, VICIA SATIVA) exceeded 100 mg/kg.

Growth: EC 50 for all three species tested (LOLIUM PERENNE, BRASSICA RAPA, VICIA SATIVA) exceeded 100 mg/kg.

No visible phytotoxic effects were observed on all three species.

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