Acetaldehyde oxime

Specific investigations: other studies

Administrative data

Endpoint:

specific investigations: other studies

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

1981

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: No GLP. No replications.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1981

Report date:

1981

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

The test substance was administered by gavaging to a beagle dog as a 2% aqueous solution followed after 4 h by 95% ethanol (0.5 mL/kg) given orally as a 25% aqueous solution. Blood pressure and heart rate measurements were carried out every 30 min post-AAO for a 4 h period. The monitoring was continued post-ethanol at 30 min intervals for a total of 165 min. Arterial samples were collected at each measurement time.

GLP compliance:

not specified

Type of method:

in vivo

Endpoint addressed:

other:

Test material

Test material information

Constituent 1

Reference substance name:

Acetaldehyde oxime

EC Number:

203-479-6

EC Name:

Acetaldehyde oxime

Cas Number:

107-29-9

Molecular formula:

C2H5NO

IUPAC Name:

acetaldehyde oxime

Details on test material:

- test material: acetaldehyde oxime (AAO)

Test animals

Species:

dog

Strain:

Beagle

Sex:

male

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Marshall Research Animals Inc., North Rose, NY, USA
- Age: 9-10 months
- Weight: 11.4 - 14 kg
- Housing: individually in elevated standard size dog cages
- Diet: 400 g of standard dog chow (Wayne Feeds) at 10 am each day and uneaten food removed 4.5 h later
- Water: ad libitum

ENVIRONMENTAL CONDITIONS
- Temperature (°C):22±3°C
- Humidity (%): 50±5%
- Air changes (per hr): 12
- Photoperiod (hrs dark / hrs light): 12/12

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

water

Analytical verification of doses or concentrations:

no

Doses / concentrations

Doses / concentrations 1

Remarks:

Doses / Concentrations:
100 mg/kg AAO
Basis:
actual ingested

Doses / concentrations 2

Remarks:

Doses / Concentrations:
0.5 mL/kg 95% ethanol
Basis:
actual ingested

No. of animals per sex per dose:

1

Control animals:

no

Examinations

Examinations:

electrocardiogram, systemic arterial blood pressure, heart rate, respiration rate, body temperature, blood sampling and analysis

Applicant's summary and conclusion

Conclusions:

Oral acetaldehyde oxime (AAO) given alone or in combination with ethanol had no dramatic effects on the cardiovascular dynamics of the conscious dog. But moderate changes were observed. There was a gradual increase in heart rate following AAO alone and there occurred a slight fall in blood pressure when AAO was followed by ethanol. Acetaldehyde blood levels were not elevated following the administration of AAO in combination with ethanol.

Executive summary:

A beagle dog was used to study the effects of oral acetaldehyde oxime (AAO) alone and in combination with ethanol. Measurements of the responses to AAO alone were made over a period of 4 h at the end of which ethanol at 0.5ml/kg diluted as a 25% aqueous solution was given orally and the studies continued for 3 h. There was essentially no overall change in the systemic blood pressure during the 4 h post AAO study period. However, the heart rate showed a progressive increase from a baseline value of about 120 beats/minute up to 180 beats/minute at 3 h. By extending this period to 4 h, the investigaters had hoped to better establish the validity of the elevated heart rate. It turned out that at the end of 4 h the heart rate was unchanged (at 180 beats/minute). Results of measurements made following ethanol administration showed that over the 3 h study period there were essentially no changes in heart rate but blood pressure did tend to decrease slightly from values obtained with AAO alone prior to administration of ethanol.