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Environmental fate & pathways

Biodegradation in water and sediment: simulation tests

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Administrative data

Endpoint:
biodegradation in water: simulation testing on ultimate degradation in surface water
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2002
Reliability:
1 (reliable without restriction)
Justification for type of information:
Read-across from the analogue substance RDX. Please see the RA justification document in section 13.

Data source

Reference
Reference Type:
publication
Title:
Unnamed
Year:
2002

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
EPA OPPTS 835.5154 (Anaerobic Biodegradability in the Subsurface)
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
Perhydro-1,3,5-trinitro-1,3,5-triazine
EC Number:
204-500-1
EC Name:
Perhydro-1,3,5-trinitro-1,3,5-triazine
Cas Number:
121-82-4
Molecular formula:
C3H6N6O6
IUPAC Name:
Perhydro-1,3,5-trinitro-1,3,5-triazine

Study design

Oxygen conditions:
anaerobic
Inoculum or test system:
activated sludge, adapted
Duration of test (contact time):
>= 36 d

Results and discussion

% Degradation
% Degr.:
72.3
St. dev.:
0.8
Parameter:
CO2 evolution
Sampling time:
36 d
Transformation products:
yes
Identity of transformation productsopen allclose all
No.:
#1
Reference
Reference substance name:
Unnamed
IUPAC name:
Methylenedinitramine
No.:
#2
Reference
Reference substance name:
Unnamed
IUPAC name:
hexahydro-1-nitroso-3,5-dinitro-1,3,5-triazine
Identifier:
common name
Identity:
hexahydro-1-nitroso-3,5-dinitro-1,3,5-triazine
No.:
#3
Reference
Reference substance name:
Unnamed
IUPAC name:
hexahydro-1,3-dinitroso-5-nitro-1,3,5-triazine
Identifier:
common name
Identity:
hexahydro-1,3-dinitroso-5-nitro-1,3,5-triazine
No.:
#4
Reference
Reference substance name:
Unnamed
IUPAC name:
1,3,5-trinitroso-1,3,5-triazinane
Inventory number:
InventoryMultipleMappingImpl [inventoryEntryValue=EC 237-766-2]
CAS number:
13980-04-6
No.:
#5
Reference
Reference substance name:
Unnamed
IUPAC name:
formaldehyde
Inventory number:
InventoryMultipleMappingImpl [inventoryEntryValue=EC 200-001-8]
CAS number:
50-00-0
Identity:
Formaldehyde
Molecular formula:
CH2O
Molecular weight:
30.026
SMILES notation:
C=O
InChl:
InChI=1/CH2O/c1-2/h1H2
No.:
#6
Reference
Reference substance name:
Unnamed
IUPAC name:
methanol
Inventory number:
InventoryMultipleMappingImpl [inventoryEntryValue=EC 200-659-6]
CAS number:
67-56-1
Identity:
Methanol
Molecular formula:
CH4O
Molecular weight:
32.042
SMILES notation:
CO
InChl:
InChI=1/CH4O/c1-2/h2H,1H3
No.:
#7
Reference
Reference substance name:
Unnamed
IUPAC name:
Carbon dioxide
Inventory number:
InventoryMultipleMappingImpl [inventoryEntryValue=EC 204-696-9]
CAS number:
124-38-9
Identifier:
common name
Identity:
Carbonic acid gas
Molecular formula:
CO2
Molecular weight:
ca. 44
SMILES notation:
O=C=O
InChl:
InChI=1S/CO2/c2-1-3
No.:
#8
Reference
Reference substance name:
Unnamed
IUPAC name:
nitrous oxide

Applicant's summary and conclusion

Validity criteria
Validity criteria fulfilled:
yes
Conclusions:
Klebsiella pneumoniae demonstrated an initial denitration followed by ring cleavage and decomposition, whereas the second strain, Clostridium bifermentans, induced an initial reduction of RDX to MNX, followed by denitration and decomposition. Both strains produced a similar product distribution including MEDINA, N2O, HCHO, MeOH, and CO2.
Executive summary:

HMX and RDX have been demonstrated to be very similar in structure, physical/chemical properties and toxicological profile in the Analogue Approach - Read Across High Melting Explosive (HMX) (2013) document (attached in section 13). It is expected that HMX and RDX have nearly the same environmental fate and behaviour. Therefore, a read-across from HMX to data obtained with RDX is scientifically justified.