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Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
5.1. - 3.2. 2004
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2004
Report date:
2004

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
Deviations:
no
Qualifier:
according to guideline
Guideline:
EU Method C.4-E (Determination of the "Ready" Biodegradability - Closed Bottle Test)
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
(3S,5S)-5-[(1S,3S)-1-azido-3-{[4-methoxy-3-(3-methoxypropoxy)phenyl]methyl}-4-methylpentyl]-3-(propan-2-yl)oxolan-2-one
EC Number:
608-748-5
Cas Number:
324763-46-4
Molecular formula:
C25 H39 N3 O5
IUPAC Name:
(3S,5S)-5-[(1S,3S)-1-azido-3-{[4-methoxy-3-(3-methoxypropoxy)phenyl]methyl}-4-methylpentyl]-3-(propan-2-yl)oxolan-2-one

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
sewage, predominantly domestic (adaptation not specified)
Details on inoculum:
- Source of inoculum/activated sludge: sewage from the output of the second stage of a sewage treatment plant
Duration of test (contact time):
28 d
Initial test substance concentration
Initial conc.:
3.64 - 3.65 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
O2 consumption
Details on study design:
TEST CONDITIONS
- Test temperature: 20 +/- 1 °C
- Continuous darkness: yes

TEST SYSTEM
- Number of culture flasks/concentration: 2x 9

SAMPLING
- Sampling frequency: Day 0, 3, 7, 10, 14, 17, 21, 24, 28

CONTROL AND BLANK SYSTEM
- Inoculum blank: 2x 9 replicates
- Reference substance: 2x 9 replicates
- Toxicity control: 2x 5 replicates
- 9 replicates for determination of oxidized nitrogen forms

Reference substance
Reference substance:
benzoic acid, sodium salt

Results and discussion

% Degradation
Parameter:
% degradation (O2 consumption)
Value:
0
Sampling time:
28 d

BOD5 / COD results

Results with reference substance:
Degradation kinetic:
Day 0: 0%
Day 3: 60.3%
Day 7: 78.8%
Day 28: 85%

Applicant's summary and conclusion

Interpretation of results:
under test conditions no biodegradation observed
Conclusions:
In this 28 day study of ready biological degradability the decomposition of 0 % of the test substance was attained.
The test substance is not readily biodegradable.

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