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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2006
Report date:
2006

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
Qualifier:
according to guideline
Guideline:
ISO 8692 (Water Quality - Fresh Water Algal Growth Inhibition Test with Scenedesmus subspicatus and Selenastrum capricornutum)
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
3,5,7-triaza-1-azoniatricyclo[3.3.1.13,7]decane nitrate
EC Number:
687-538-5
Cas Number:
18423-20-6
Molecular formula:
C6H13N5O3
IUPAC Name:
3,5,7-triaza-1-azoniatricyclo[3.3.1.13,7]decane nitrate
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
Not avalaible

Sampling and analysis

Analytical monitoring:
not specified
Details on sampling:
Not avalaible

Test solutions

Vehicle:
not specified
Details on test solutions:
Not avalaible

Test organisms

Test organisms (species):
Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
Details on test organisms:
TEST ORGANISM
- Common name:Selenastrum capricornutum (new name: Pseudokirchnerella subcapitata)
- Source (laboratory, culture collection): culture collection CCAP "Culture collection of Algae and Protozoa, ref. 278/4

ACCLIMATION
- Acclimation period: 3 or 4 days

Study design

Test type:
not specified
Water media type:
freshwater
Total exposure duration:
72 h
Post exposure observation period:
Not available

Test conditions

Hardness:
Not available
Test temperature:
23 (+/-) 2ºC
pH:
pH (control) (0 horas): 7.8
pH (control) (72 horas): 9.3
pH (100 %) (0 horas): 7.4
pH (100 %) (72 horas): 7.7
Dissolved oxygen:
Not available
Salinity:
Not avalaible
Nominal and measured concentrations:
100 mg/L, 50 mg/L, 25 mg/L, 12.5 mg/L y 6.25 mg/L
Details on test conditions:
Not avalaible
Reference substance (positive control):
not specified

Results and discussion

Effect concentrationsopen allclose all
Duration:
72 h
Dose descriptor:
IC50
Effect conc.:
ca. 43.4 mg/L
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
ca. 12.5 mg/L
Duration:
72 h
Dose descriptor:
LOEC
Effect conc.:
ca. 25 mg/L
Details on results:
Not avalaible
Results with reference substance (positive control):
Not avalaible

Any other information on results incl. tables

   Control  6.25 mg/l 12.5 mg/l  25 mg/l  50 mg/l  100 mg/l 
 %Inhibición (72h)  0.0 9.97  3.01  14.70  62.66  99.78 

Applicant's summary and conclusion

Conclusions:
CE50b (72 h) (Selenastrum capricornutum): 43.4 mg/L.
Not classified as Acute 1.

This data will be used for chronic toxicity classification.
Executive summary:

N/Ref. 1555 CE50b (72 h) Intervalo de confianza 95% (mg/L)
S/Ref. Sol. Nitrato de Hexamina 43.4 mg/L 40.6 -46.0
NOAC2: 12.5 mg/L LOEC3: 25.0 mg/L