α,α'-propylenedinitrilodi-o-cresol

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2012

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes

Analytical monitoring:

yes

Vehicle:

no

Details on test solutions:

- 40 mg of the test item was weighed into a glass flask and quantitatively transferred into a volumetric flask by multiple washing with the test medium in a total volume of 1000 mL.
- After a thorough mixing the stock mixture was not homogeneous with visible not dissolved parts of the test item.
- Therefore, the stock mixture in a tightly closed flask was sonicated with heating at 40°C for 20 min and then mixed by mechanic shaker at room temperature for 72 h.
- The homogeneous and transparent stock solution of yellow colour and characteristic odour was used as the highest test concentration and was used to prepare the lower test concentrations by sequential dilution with the test medium.
- The solutions of the test item were homogeneous, transparent of yellow colour and characteristic odour and no changes in appearance were recorded during the test.

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna Straus
- Source: Institute of Industrial Organic Chemistry Branch Pszczyna, Department of Ecotoxicology, Laboratory of Aquatic Toxicology
- Age at study initiation (mean and range, SD): < 24 h old at test initiation
- Feeding during test: no
- Food type: Pseudokirchneriella subcapitata algae suspension

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test temperature:

20.8 – 22.7 ºC

pH:

Fresh solution at exposure initiation: 7.60 - 8.15
Old solutions at renewal: 7.70 - 8.11

Dissolved oxygen:

Fresh solution at exposure initiation: 7.07 - 8.60
Old solutions at renewal: 7.51 - 8.30

Nominal and measured concentrations:

Nominal test concentrations: control, 2.5, 5, 10, 20 and 40 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: glass beakers covered with transparent lids
- Size, fill volume: 150 mL, 50 mL
- Aeration: no
- Renewal rate of test solution: renewal of old solutions for fresh solutions after 24 h of exposure
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

OTHER TEST CONDITIONS
- Photoperiod: 16 h light : 8 h darkness

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

3.162 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

1.77 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

LOEC

Effect conc.:

2.87 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

5.034 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

2.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

- In samples of fresh solutions the concentration of the test item was in the range of 80.0 – 92.8% of nominal.
- In samples of old solutions the determined concentration of the detected substance was in the range of 25.0 – 82.2% of initial.
- The concentration of the test item was not stable under test conditions.

Results with reference substance (positive control):

The EC50 (48h) of the control substance potassium dichromate was 0.723 mg/L.

Immobilization of Daphnia magna in control was 5% (allowed not more than 10%).

Oxygen concentration in the test vessels was between 7.07 – 8.60 mg/L (allowed not less than 3 mg/L).

Validity criteria fulfilled:

yes

Conclusions:

The acute toxicity of aquatic invertebrates was investigated for ‘α,α'-propylenedinitrilodi-o-cresol’ with the waterflea Daphnia magna in a GLP guideline study according to OECD 202. 

After 48 hours exposure in a semi-static test system the EC50 was 3.162 mg/L based on measured test substance concentration.

Description of key information

Acutely toxic to aquatic invertebrates.

The acute toxicity of aquatic invertebrates was investigated with the waterflea Daphnia magnas in a GLP guideline study according to OECD 202.

After 48 hours exposure in a semi-static test system the EC50 was 3.162 mg/l based on measured test substance concentration.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

3.162 mg/L