2-chloro-5-(trifluoromethyl)pyridine

Acute Toxicity: other routes

Administrative data

Endpoint:

acute toxicity: other routes

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1984

Report date:

1984

Materials and methods

GLP compliance:

yes

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

2-chloro-5-(trifluoromethyl)pyridine

EC Number:

257-856-5

EC Name:

2-chloro-5-(trifluoromethyl)pyridine

Cas Number:

52334-81-3

Molecular formula:

C6H3ClF3N

IUPAC Name:

2-chloro-5-(trifluoromethyl)pyridine

Constituent 2

Reference substance name:

2-chloro-5-(trifluoromethyl) pyridine

IUPAC Name:

2-chloro-5-(trifluoromethyl) pyridine

Details on test material:

2-chloro-5-trifluoromethyl pyridine CTL Number Y02424/003/001, is a white solid with melting point 32°C and boiling point 152°C and a stated purity of >98%w/w. It was supplied by Mond Division, Imperial Chemical Industries PLC, Runcorn Heath, Cheshire UK.

Test animals

Species:

mouse

Strain:

C57BL

Sex:

male/female

Details on test animals or test system and environmental conditions:

Male and femal C57BL/6J mice (6-8 weeks old) were used for both phase I (LD50/7day) and Phase II (Mouse Micronucleus) of the study. The animals were supplied by the Animal Breeding Unit, Alderley Park, Macclesfield, Cheshire.
On arival the mice were housed ten per cage on mobile mouse racks and given food (Porton Combined Diet (PCD) supplied by Special Diets Services Ltd, Stepfield, Witham, Essex, UK) and filtered tap water (via an automated watering system) ad libitum.
The temperature of the animal cell was maintained in the range 20-26°C and a relative humidity range of 36-65%. Temperature and relative humidity were measured on a maximum and minimum thermometer and hygrometer respectively, and were recorded in the CTL Cleanside Temperature Record Book. Lighting was cycled with 12 hours light and dark each 24 hours. The animal cell was under negative pressure with respect to the access corridor and had approximately 22 air changes per hour.

Administration / exposure

Route of administration:

intraperitoneal

Vehicle:

corn oil

Details on exposure:

2-chloro-5(trifluoromethyl) pyridine was administered as a single intraperitoneal dose to groups of five male and five female mice at the dose levels below. The animals were observed daily for deaths over a seven day period.

Doses:

100, 250, 500, 750, 1000mg/kg/bw

No. of animals per sex per dose:

5 males and 5 females

Control animals:

no

Details on study design:

The i.p. LD50/7 of CTF was determined in the mouse by calculation from the deaths observed over a seven-day observation period following single intraperitoneal dose.

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

ca. 750 mg/kg bw

Based on:

test mat.

Mortality:

2/5 males dosed at 750mg/kg/bw died within the 7 day observation period
2/5 females dosed at 1000mg/kg/bw died within the 7 day observation period

Any other information on results incl. tables

2-chloro-5(trifluoromethyl) pyridine was administered as a single intraperitoneal dose to groups of five male and five female mice at the dose levels shown. The animals were observed daily for deaths over a seven day period.

Group	Compound	Dose mg/kg	Sex	Animal Nos.	No. Deaths
6	CTF	100	M	26-30	0
			F	76-80	0
7	CTF	250	M	31-35	0
			F	81-85	0
8	CTF	500	M	36-40	0
			F	86-90	0
9	CTF	750	M	41-45	2
			F	91-95	0
10	CTF	1000	M	46-50	0
			F	96-100	2

Applicant's summary and conclusion

Conclusions:

The intraperitoneal LD50 (7 day) for CTF was determined to be 750mg/kg/bw in the mouse.

Executive summary:

2-chloro-5(trifluoromethyl) pyridine (CTF) was administered in corn oil as a single intraperitoneal dose to groups of five male and five female mice at dose levels of 100, 250, 500, 750 and 1000mg/kg/bw. The animals were observed daily for deaths over a seven day period. The intraperitoneal LD50 (7 day) for CTF was determined to be 750mg/kg/bw in the mouse.