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Toxicological information

Eye irritation

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Administrative data

Endpoint:
eye irritation: in vivo
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Remarks:
no data on test substance purity

Data source

Reference
Reference Type:
publication
Title:
Acute Toxicologic Evaluation of 1-Ethylpiperazine
Author:
Bomhard E., Pauluhn J., Ruf J.
Year:
1997
Bibliographic source:
International Journal of Toxicology, 16(2), 60 - 61

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 405 (Acute Eye Irritation / Corrosion)
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
1-ethylpiperazine
EC Number:
226-166-6
EC Name:
1-ethylpiperazine
Cas Number:
5308-25-8
Molecular formula:
C6H14N2
IUPAC Name:
1-ethylpiperazine
Details on test material:
- Name of test material (as cited in study report): 1 (N)-Ethylpiperazine
- Analytical purity: no data
No further information available.

Test animals / tissue source

Species:
rabbit
Strain:
New Zealand White
Details on test animals or tissues and environmental conditions:
TEST ANIMALS
- Weight at study initiation: 3.1 - 3.4 kg
No further information.

Test system

Vehicle:
unchanged (no vehicle)
Controls:
yes, concurrent no treatment
Amount / concentration applied:
TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 100 µL
Duration of treatment / exposure:
24 h
Observation period (in vivo):
7 days
Number of animals or in vitro replicates:
3
Details on study design:
REMOVAL OF TEST SUBSTANCE
- Washing (if done): rinsed with physiologic saline
- Time after start of exposure: 24 h

SCORING SYSTEM: according to Draize; the irritation indices were calculated in accordance with Guideline 83/467/EC No. 8

Results and discussion

In vivo

Resultsopen allclose all
Irritation parameter:
cornea opacity score
Basis:
mean
Time point:
other: 24 - 48 - 72 h
Score:
3
Max. score:
4
Reversibility:
not specified
Irritation parameter:
iris score
Basis:
mean
Time point:
other: 24 - 48 - 72 h
Score:
1
Max. score:
2
Reversibility:
not specified
Irritation parameter:
conjunctivae score
Basis:
mean
Time point:
other: 24 - 48 - 72 h
Score:
3
Max. score:
3
Reversibility:
not specified
Irritation parameter:
chemosis score
Basis:
mean
Time point:
other: 24 - 48 - 72 h
Score:
3
Max. score:
4
Reversibility:
not specified
Irritant / corrosive response data:
After 7 d the nictitating membrane and the palpebral conjunctiva showed necrotic changes. Therefore, the test substance was found to cause severe damage to the eyes.

Applicant's summary and conclusion

Interpretation of results:
Category 1 (irreversible effects on the eye) based on GHS criteria