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Short-term toxicity to fish

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Description of key information

Key value for chemical safety assessment

LC50 for freshwater fish:
2 mg/L

Additional information

In the Klimisch 1 GLP study from Kimmel (2013) the acute toxicity of Sulfosuccinic (FA C12-18)E, 2Na to zebra fish (Danio rerio) was determined in a 96‑hour semi-static test with daily test medium renewal according to the EU Commission Directive 92/69/, Part C.1, the Commission Regulation (EC) No 440/2008, Part C.1 and the OECD Guideline for Testing of Chemicals No. 203 (1992). The following nominal concentrations were tested: 0.15, 0.47, 1.5, 4.7 and 15 mg/L mg test item/L. Additionally, a control (test water without test item) was tested in parallel.

The measured concentrations of the test item in the test media of the test concentrations of 0.15 to 15 mg/L were between 81 and 112% of the nominal values at the start of the test and below Limit of Quantification (LOQ) to 109% at the end of each renewal period. Thus, the correct dosage of the test item was confirmed, but the test item concentrations of the two lowest concentrations declined below 80% over the renewal period of 24 hours. Therefore the biological results were related to the following mean measured test item concentrations: control, 0.090, 0.33, 1.6, 4.8 and 16 mg test item/L.

In the control and at the test concentrations up to and including 0.090 mg/L, all fish survived until the end of the test and no visible abnormalities were observed in the test fish. At the next higher test concentration of 0.33 mg/L, three test fish showed one or several visible abnormalities. One of the seven test fish died at this test concentration. At the test concentration of 4.8 mg/L all test fish were dead after 48 hours of test duration while at the highest test concentration of 16 mg/L, all fish were dead already after 24 hours of test duration. Thus, the 96‑hour NOEC and LC0of Sulfosuccinic (FA C12-18)E, 2Na to zebra fish were both determined to be 0.090 mg/L. The 96‑hour LC50of Sulfosuccinic (FA C12-18)E, 2Na was calculated to be 2.0 mg/L with a 95% confidence interval of 0.8-3.3 mg/L. The 96‑hour LC100was 4.8 mg/L.

The results of this study are considered relevant and reliable for the risk assessment.