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Short-term toxicity to fish

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short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2011-12-05 - 2011-12-12
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
according to
OECD Guideline 203 (Fish, Acute Toxicity Test)
GLP compliance:
Specific details on test material used for the study:
- Name of test material (as cited in study report): 1,1-[[3-(dimethylamino)propyl]imino]bis(2-propanol)
- Substance type: organic
- Physical state: clear amber liquid
- Analytical purity: 85.7%
- Impurities (identity and concentrations): not reported
- Lot/batch No.: PFW100241
- Storage condition of test material: at ambient temperature in original container
Analytical monitoring:
Details on sampling:
- Samples for test substance concentration verification were taken at 0 and 96 h.
Details on test solutions:
- A 120 mg/L test solution of 1,1’-[[3-(DIMETHYLAMINO)PROPYL]IMINO]BIS(2-PROPANOL) was prepared by adding a nominal solution of 1.2 g to 10 L of dilution water. The test solution was stirred vigorously for 15 minutes resulting in a clear colourless solution. The pH of the test solution was adjusted from 10.1 to 7.7 using 1M HCl.
- Dilution water: tap water which had been coarsely filtered to remove particulate material, passed through activated carbon and dechlorinated using sodium thiosulphate. Salts were added, as required, to maintain minimum hardness levels, and the treated water passed through an ultraviolet steriliser and filtered to 10 μm.
Test organisms (species):
Oncorhynchus mykiss (previous name: Salmo gairdneri)
Details on test organisms:
- Common name: rainbow trout
- Source: Houghton Springs Fish Farm, Winterbourne Houghton, Blandford Forum, Dorset, DT11 0PD
- Age at study initiation (mean and range, SD): not reported (juvenile)
- Length at study initiation (length definition, mean, range and SD): not reported
- Weight at study initiation (mean and range, SD): not reported
- Feeding during test: no

- Acclimation period: approx 4 wks
- Acclimation conditions (same as test or not): same as test
- Type and amount of food: commercial fish food (amount not specified)
- Feeding frequency: daily
- Prophylactic treatment: a twice daily treatment of formalin and malachite green which was completed > 7 days before the start of the test (upon arrival until finalisation of this treatment fish were also held in 20% seawater)
- Health during acclimation (any mortality observed): no mortalities recorded within 7 days prior to the start of the study
Test type:
Water media type:
Limit test:
Total exposure duration:
96 h
In the dechlorinated water supply: 61.0 mg/L expressed as CaCO3
Test temperature:
14.5 - 15.0 °C
7.35 - 7.86
Dissolved oxygen:
7.85 - 10.29 mg/L
Not applicable
Nominal and measured concentrations:
Nominal: 0, 120 mg/L
Measured: < 1.0, 130 mg/L at both t=0 and t= 96h
Details on test conditions:
- Test vessel: glas vessels
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: glass, 10 L, fill volume not reported
- Aeration: yes, gently
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1

- Source/preparation of dilution water: as described under "details on test solution"
- Total organic carbon: 2.49 mg/L
- Particulate matter: <3 mg/L
- Metals: not reported
- Pesticides: < 0.05 μg/L (organo chlorine), < 0.01 μg/L (organo phosphorus)
- Chlorine: < 2 μg/L, as Cl2, free as well as total
- Alkalinity: 30.2 mg/L as CaCO3
- Ca/mg ratio: not reported
- Conductivity: 233 μS/cm
- Intervals of water quality measurement: pH and conductivity daily, alkalinity, hardness and free & total chlorine once per week, other parameters quarterly.
- During the test, pH, temperature, and dissolved oxygen concentrations were measured daily.

- Adjustment of pH: from 10.1 to 7.7 using 1M HCl
- Photoperiod: 16h day, 8h night, 20 min dawn
- Light intensity: not reported

EFFECT PARAMETERS MEASURED (with observation intervals if applicable): mortality (at 3, 24, 48, 72 and 96 h)

- Test concentrations: 0 (dilution water) and 120 mg/L
Key result
96 h
Dose descriptor:
Effect conc.:
> 120 mg/L
Nominal / measured:
Conc. based on:
test mat.
Basis for effect:
Details on results:
- Behavioural abnormalities: none
- Observations on body length and weight: control fish at end of exposure period: weight: range 0.75 - 1.18 g, mean 0.94 g; length: range 40 - 48 mm, mean 44 mm
- Other biological observations: none
- Mortality of control: no
- Other adverse effects control: none
- Abnormal responses: none
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: no
- Effect concentrations exceeding solubility of substance in test medium: no
Reported statistics and error estimates:
Validity criteria fulfilled:
Juvenile rainbow trout (Oncorhynchus mykiss) was exposed to the test substance at 0 and 120 mg/L (limit test). No mortality was observed and the 96-h LC50 is concluded to be > 120 mg/L. Measured concentrations were in agreement with nominal concentrations.

Description of key information

A limit test was performed under GLP conditions and according to OECD TG 203.

An LC50 was found for Rainbow trout (Oncorhynchus mykiss) of > 120 mg/L after an exposure of 96 hours in a static test (Vaughan et al., 2012).

Key value for chemical safety assessment

Additional information

A reliable (K1) study is available to cover this endpoint.