1-hydroxy-4-methyl-6-(2,4,4-trimethylpentyl)pyridin-2(1H)-one, compound with 2-aminoethanol (1:1)

Other distribution data

Administrative data

Endpoint:

other distribution data

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2004-07-14 - 2004-08-13

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Test was carried out according established internal method

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2004

Report date:

2004

Materials and methods

Principles of method if other than guideline:

Measuring the elimination of Octopirox from a buffered aqueous solution by adding an aqueous Fe(III) solution in 10 fold excess

GLP compliance:

no

Type of study:

other: Complexation of Octopirox by Fe(III) ions

Media:

other: test item and Fe(III) dissolved in water

Test material

Test material information

Constituent 1

Reference substance name:

1-hydroxy-4-methyl-6-(2,4,4-trimethylpentyl)pyridin-2(1H)-one, compound with 2-aminoethanol (1:1)

EC Number:

272-574-2

EC Name:

1-hydroxy-4-methyl-6-(2,4,4-trimethylpentyl)pyridin-2(1H)-one, compound with 2-aminoethanol (1:1)

Cas Number:

68890-66-4

Molecular formula:

C14H23NO2.C2H7NO

IUPAC Name:

1-hydroxy-4-methyl-6-(2,4,4-trimethylpentyl)pyridin-2(1H)-one, compound with 2-aminoethanol (1:1)

Constituent 2

Reference substance name:

Octopirox

IUPAC Name:

Octopirox

Details on test material:

as described in IUCLID Section 1

Results and discussion

Any other information on results incl. tables

Recovery experiment:

15,0 mg Octopirox® were dissolved in 100 ml water. Three times each 10 ml of the standard solution were percolated through RP-8 solid phase extraction cartridges (6 ml/1000 mg adsorbent). After washing with approx. 70 ml water the cartridges were sucked to dryness and eluted with 10 ml methanol. 10 µl titanium(III) chloride were added to 1 ml of each methanol eluate and volumes were made up to 5 ml with methanol. This final solutions were injected into the HPLC instrument.

 

Sample	Recovery rate [%]
Recov. 1	94,9
Recov. 2	93,8
Recov. 3	94,0
mean	94,2

Result

 The elimination rate caused by complexation with iron(III) ions is³99,7 %.

 

	initial Octopirox® concentration [µg/L]	Octopirox® concentration after 24 h contact with iron(III) [µg/L]	Octopirox® concentration after 48 h contact with iron(III) [µg/L]
Trial A	18000	60	282*
Trial B	18000	54	39
Trial C	18000	46	39

 

*: outlier, probably caused by filtration problems.

 

Applicant's summary and conclusion

Executive summary:

Complextion of Octopriox with di- and trivalent ions like e.g. Fe(II), Fe(III), Ca(II), Al(III) reduces the freely available Octopirox concentration considerable. This means ecotoxicity relevant for an environmental safety assessment is reduced as well.