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Ecotoxicological information

Toxicity to microorganisms

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Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
July 10 to 16 2009
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2009
Report Date:
2009

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
Deviations:
yes
Remarks:
use of copper(II)sulphate pentahydrate as referrence item instead of 3,5-dichlorophenol
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): 2-Octyl-1-dodecanol
- Substance type: pure active compound
- Physical state: liquid
- Lot/batch No.: 03508
- Expiration date of the lot/batch: January 1, 2011
- Storage condition of test material: room temperature, protected from moisture and light

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The tets substance was weighed out directly into an Erlenmeyer flask and flled up with 250 ml autoclaved tap water and stirred for 24 hours.
- Controls: 2 control samples without test item were included, one at the beginnning and one at the end of the test
- Evidence of undissolved material (e.g. precipitate, surface film, etc): The test item formed drops and swam on the surface of the test solution.

Test organisms

Test organisms (species):
activated sludge of a predominantly domestic sewage
Details on inoculum:
- Pretreatment: The sludge was washed twice with autoclaved tap water and adjusted corresponding to a dry weight of 4.0 g/l +/- 10%
- Initial biomass concentration: 3.83 g/l dry sludge concentration

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
3 h

Test conditions

Test temperature:
18-22°C
pH:
6.72
Nominal and measured concentrations:
1000 mg/l (nominal)
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: 500 ml erlenmeyer flasks, DIN 12380
- Aeration: permanent
- No. of vessels per concentration (replicates): one
- No. of vessels per control (replicates): two
- Composition of tes medium: 200 ml inoculum, 16 ml synthetic waste water, 284 ml autoclaved tap water and test item concentration

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : oxygen depletion by means of an oximeter

TEST CONCENTRATIONS
- Range finding study
- Test concentrations: 10, 100 and 1000 mg/l
- Results used to determine the conditions for the definitive study: no inhibition at any of the concentrations tested
Reference substance (positive control):
yes
Remarks:
copper (II) sulphate pentahydrate p.a.

Results and discussion

Effect concentrations
Key result
Duration:
3 h
Dose descriptor:
EC0
Effect conc.:
>= 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth inhibition
Results with reference substance (positive control):
- Results with reference substance valid? Yes
- Relevant effect levels: EC50 = 99 mg/l

Any other information on results incl. tables

Table 1: respiration rates and Inhibition of Respiration of Test and Reference Item Concentrations
  concentration [mg/l] Respiration rate [mgO2/l*h] Inhibition [%]
Test item 1000 20 0
Control 1 - 20.4 -
Control 2 - 19.6 -
reference item 180 4 80
  100 9.6 52
  58 15.6 22

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
2-Octyldodecan-1-ol is not toxic at concentrations <= 1000 mg/l to activated sludge of a municipal sewage treatment plant.
Executive summary:

2-Octyldodecan-1-ol is not toxic at concentrations <= 1000 mg/l to activated sludge of a municipal sewage treatment plant.