Registration Dossier

Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
From August 30 to September 3, 2011
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2011
Report date:
2011

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Qualifier:
according to guideline
Guideline:
other: EN 14735:2005 Characterization of waste-preparation of waste sample for ecotoxicity tests.
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
solid: particulate/powder
Remarks:
migrated information: powder
Details on test material:
Product: CarboNeXT
Physical state: solid
Batch: 08-07-2011

Test organisms

Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
14 fishes have been used, 7 of them treated with the extract of the test substance, the other 7 fishes were used as control.

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
96 h

Test conditions

Hardness:
245 mg CaCO3/L
Test temperature:
23+/-2°C
pH:
pH has remained within required limits (6.0 - 8.5).
Nominal and measured concentrations:
100 mg/l
Details on test conditions:
Medication: Animals, 7 for group, have been added to the assay tanks with a biomass density lower than 1 g/L.
Exposition: The treated group and the control group have been observed for 96 hours with a photoperiod of 12 hours of light and 12 hours of darkness.

Results and discussion

Effect concentrations
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)

Any other information on results incl. tables

Sublethal observations / clinical signs:

Mortality

No case of mortality has been detected in treated animals and in control ones:

Symptomatology

No toxic symptom has been detected in treated animals and in control ones:

% of oxygen saturation

The percentage of saturation has remained for the whole length of the assay above 60% both in control and in treated.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The obtained results, in compliance with assay validity criteria, shows that n dead fishes at the concentration of 100 mg/L of the extract of the test substance after 96 hours were observed; the LC50 value after 96 hours results higher than 100 mg/L of the extract of the test substance.