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Environmental fate & pathways

Biodegradation in water: screening tests

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Description of key information

Within 28 days a degradation of 75 % for bis(2-ethylhexyl) hydrogen phosphate was determined according to the OECD Guideline 301 F. Therefore bis(2-ethylhexyl) hydrogen phosphate is considered as readily biodegradable.

Key value for chemical safety assessment

Biodegradation in water:
readily biodegradable

Additional information

To test for its biodegradability potential, bis(2-ethylhexyl) hydrogen phosphate was incubated for 28 days in continuously stirred 250 ml closed flask in the dark with an inoculum originally collected from a local, predominantly municipal wastewater treatment plant. The applied method was the OECD Guideline 301 F. In this assay, biodegradation was estimated by biological oxygen demand (BOD) over time. BOD was measured daily by a manometer. The incubation temperature was 20 +/-1°C. The concentration of innoculum was 30 mg /L and the one of the substance was 100 mg/L. Degradation was calculated by substracting the amount BOD in the negative (innoculum only) control from that in the substance or positive control at any given time point and divided by the chemical oxygen demand (COD) or thereotical oxygen demand (ThOD). The 28-day degradation was 75% forbis(2-ethylhexyl) hydrogen phosphate and 82% for the positive control (with reference substance).

A second test on ready biodegradability was conducted according to the national Japanese standard method comparable to the OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I)). After 14 days, the substance showed 0-17% degradation realting to BOD, and 0% degradation relating to the test substance concentration.

As recommended in Guidance R.7b (ECHA, 2012), in cases of conflicting test results, differences in stringency and the origin of the inoculum in order to check whether or not differences in the adaptation of the inoculum may be the reason are considered. The reported data are carefully checked and no differences in test design were found in both tests. The positive result in the well documented study for ready biodegradability is considered as indicative of rapid and ultimate degradation and the positive test result supersede the negative test result.

In conclusion the bis(2-ethylhexyl) hydrogen phosphate is considered as readily biodegradable.