Bis(2-ethylhexyl) hydrogen phosphate

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: scientifically acceptable and sufficient documented - but only 2 doses applied

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1982

Report date:

1982

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

Principles of method if other than guideline:

Two groups of 5 male and 5 female rats were dosed once by oral gavage at dose levels of 500 or 5000 mg/kg bw. The animals were observed for mortality, body weight and clinical signs through day 14. After 14 days, the surviving animals were killed and discarded.

GLP compliance:

not specified

Test type:

standard acute method

Test material

Test material information

Constituent 1

Reference substance name:

Bis(2-ethylhexyl) hydrogen phosphate

EC Number:

206-056-4

EC Name:

Bis(2-ethylhexyl) hydrogen phosphate

Cas Number:

298-07-7

Molecular formula:

C16H35O4P

IUPAC Name:

bis(2-ethylhexyl) hydrogen phosphate

Test material form:

other: liquid

Details on test material:

no data

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

unchanged (no vehicle)

Doses:

500 or 5000 mg/kg bw

No. of animals per sex per dose:

5 male and 5 female rats/dose

Control animals:

not specified

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 500 - < 5 000 mg/kg bw

Based on:

test mat.

Any other information on results incl. tables

All animals doses at 5000 mg/kg bw died within 24 hours after application of bis(2-ethylhexyl) hydrogen phosphate and were necropsied. Severly redness abdominal walls, enlarged blood vessels, reddened liningof the gastrointestinal tract were found at necrosy. The stomach lining had a dark red clour. Adrenals and pancreas were reddened. Lungs in most instances were found to be scarlet red, kidneys were pale and thymus darkened.

All animals dosed with 500 mg/kg bw survived 14 days and gained weight through the study. Clinically, the animals exhibited oral/nasal discharge and diarrhea on the day of dosing only.

Applicant's summary and conclusion

Executive summary:

Two groups of 5 male and 5 female rats were dosed once by oral gavage of bis(2-ethylhexyl) hydrogen phosphate at dose levels of 500 or 5000 mg/kg bw. The animals were observed for mortality, body weight and clinical signs through day 14. After 14 days, the surviving animals were killed and discarded.

All animals doses at 5000 mg/kg bw died within 24 hours after application of bis(2-ethylhexyl) hydrogen phosphate and were necropsied. Severly redness abdominal walls, enlarged blood vessels, reddened liningof the gastrointestinal tract were found at necrosy. The stomach lining had a dark red clour. Adrenals and pancreas were reddened. Lungs in most instances were found to be scarlet red, kidneys were pale and thymus darkened.

All animals dosed with 500 mg/kg bw survived 14 days and gained weight through the study. Clinically, the animals exhibited oral/nasal discharge and diarrhea on the day of dosing only.

An oral LD50 > 500 and < 5000 mg/kg bw was determined.