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Toxicological information

Skin irritation / corrosion

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Administrative data

Endpoint:
skin irritation: in vivo
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: scientifically acceptable and sufficient documented

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1978
Report date:
1978

Materials and methods

Principles of method if other than guideline:
500 µl/animal of bis(2-ethylhexyl) hydrogen phosphate was applied to the inner surface of one ear per animal; exposure time was 1, 4, or 8 hour(s). The test substance was fixed by a plaster. After the exposition the test substance was removed with water and soap/plantoil. The post exposure observation time was 7 days.
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Bis(2-ethylhexyl) hydrogen phosphate
EC Number:
206-056-4
EC Name:
Bis(2-ethylhexyl) hydrogen phosphate
Cas Number:
298-07-7
Molecular formula:
C16H35O4P
IUPAC Name:
bis(2-ethylhexyl) hydrogen phosphate
Details on test material:
no data

Test animals

Species:
rabbit
Strain:
New Zealand White

Test system

Type of coverage:
semiocclusive
Preparation of test site:
other:
Controls:
not required
Duration of treatment / exposure:
1, 4, or 8 hour(s)
Observation period:
7 days
Number of animals:
1 animal/exposure period

Results and discussion

In vivo

Resultsopen allclose all
Irritation parameter:
erythema score
Basis:
animal #1
Time point:
other: 24, 48, and 72 h
Score:
1.3
Max. score:
4
Reversibility:
not fully reversible within: 7 days
Remarks on result:
other: exposure period: 1 hour
Irritation parameter:
edema score
Basis:
animal #1
Time point:
other: 24, 48, and 72 h
Score:
0
Max. score:
4
Reversibility:
other: not applicable - score = 0 at any time point
Remarks on result:
other: exposure time: 1hour
Irritation parameter:
erythema score
Basis:
animal #2
Time point:
other: 24, 48, and 72 h
Score:
2
Max. score:
4
Reversibility:
not fully reversible within: 7 days
Remarks on result:
other: exposure period: 4 hours
Irritation parameter:
edema score
Basis:
animal #2
Time point:
other: 24, 48, and 72 h
Score:
0
Max. score:
4
Reversibility:
other: not applicable - score = 0 at any time point
Remarks on result:
other: exposure period: 4 hours
Irritation parameter:
erythema score
Basis:
animal #3
Time point:
other: 24, 48, and 72 h
Score:
2.3
Max. score:
4
Reversibility:
not fully reversible within: 7 days
Remarks on result:
other: exposure period: 8 hours
Irritation parameter:
edema score
Basis:
animal #3
Time point:
other: 24, 48, and 72 h
Score:
0
Max. score:
4
Reversibility:
other: not applicable - score = 0 at any time point
Remarks on result:
other: exposure period: 8 hours
Other effects:
A surface corrosivity was observed at an application period of 4 to 8 hours.

Any other information on results incl. tables

exposure 1 hour: edema = 0 at any time point; erythema was not fully reversible within 7 days, the test substance was sligt to moderate irritant.

exposure 4 or 8 hours: edema = 0 at any time point; erythema was not fully reversible within 7 days, the test substance was strong irritating and corrosive to the skin surface.

Applicant's summary and conclusion

Interpretation of results:
corrosive
Remarks:
Migrated information
Executive summary:

500 µl/animal of bis(2-ethylhexyl) hydrogen phosphate was applied to the inner surface of one ear per animal; exposure time was 1, 4, or 8 hour(s). The test substance was fixed by a plaster. After the exposition the test substance was removed with water and soap/plantoil. The post exposure observation time was 7 days.

Exposure 1 hour: edema = 0 at any time point; erythema was not fully reversible within 7 days, the test substance was slight to moderate irritant.

Exposure 4 to 8 hours: edema = 0 at any time point; erythema was not fully reversible within 7 days, the test substance was strong irritating to the skin surface. A surface corrosivity was observed at an application period of 4 to 8 hours.