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EC number: 206-056-4 | CAS number: 298-07-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 012
- Report date:
- 2012
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Principles of method if other than guideline:
- Bis(2-ethylhexyl) hydrogen phosphate was initially investigated using the Salmonella/microsome plate incorporation test for point mutagenic effects in doses of up to and including 5 µL per plate on five Salmonella typhimurium L T2 mutants. These comprised the histidine-auxotrophic strains TA 1535, TA 100, TA 1537, TA 98 and TA 102. The independent repeat was performed as preincubation for 60 minutes at 37°C. Other conditions remained unchanged.
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Bis(2-ethylhexyl) hydrogen phosphate
- EC Number:
- 206-056-4
- EC Name:
- Bis(2-ethylhexyl) hydrogen phosphate
- Cas Number:
- 298-07-7
- Molecular formula:
- C16H35O4P
- IUPAC Name:
- bis(2-ethylhexyl) hydrogen phosphate
- Test material form:
- other: liquid
- Details on test material:
- Content: 99.3 %
Constituent 1
Method
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and TA 102
- Additional strain / cell type characteristics:
- other: Firstly, they are deep rough since certain lipopolysaccharide side chains are missing in the bacterial cell wall. Secondly, their reduced ability to repair damage from UV light (e.g. thymidine dimers) allows the phenotypic detection of mutation events
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 mix
- Test concentrations with justification for top dose:
- Plate incorporation method: with and without S9 mix: 0, 0.05, 0.16, 0.5, 1.6, 5.0 µl/plate
Preincubation method: with and without S9 mix: 0, 0.05, 0.16, 0.5, 1.6, 5.0 µl/tube
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- not specified
- Positive controls:
- yes
- Positive control substance:
- 2-nitrofluorene
- sodium azide
- cumene hydroperoxide
- mitomycin C
- other: anthracene-2-amine, 4-nitro-o-phenylenediamine
Results and discussion
Test results
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and TA 102
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- above 16 µl/plate
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- other: other: S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and TA 102
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
No evidence of mutagenic activity of bis(2-ethylhexyl) hydrogen phosphate was seen in this ames test. No biologically relevant increase in the mutant count, in comparison with the negative control, was observed up to the highest assessable doses.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative - Executive summary:
Bis(2-ethylhexyl) hydrogen phosphate was initially investigated using the Salmonella/microsome plate incorporation test for point mutagenic effects in doses of up to and including 5 µL per plate on five Salmonella typhimurium L T2
mutants. These comprised the histidine-auxotrophic strains TA 1535, TA 100, TA 1537, TA 98 and TA 102. The independent repeat was performed as preincubation for 60 minutes at 37° C. Other conditions remained unchanged.
No precipitation was observed up to the highest tested dose.
In the plate incorporation test doses of up to and including 0.16 µl per plate did not cause any bacteriotoxic effects. At higher doses, bis(2-ethylhexyl) hydrogen phosphate had a strain-specific bacteriotoxic effect. Due to this effect this range could not be entirely used up to 5 µl per plate for assessment purposes.
In the preincubation test doses of up to and including 0.16 µl per plate did not cause any bacteriotoxic effects. At higher doses, bis(2-ethylhexyl) hydrogen phosphate had a strain-specific bacteriotoxic effect. Due to this effect this range could not be entirely used up to 5 µl per plate for assessment purposes.
Evidence of mutagenic activity of bis(2-ethylhexyl) hydrogen phosphate was not seen. No biologically relevant increase in the mutant count, in comparison with the negative control, was observed up to the highest assessable doses.
The positive controls sodium azide, anthracene-2-amine, cumene hydroperoxide, mitomycin C, 2-nitro-9H-fluorene and 4-nitro-o- phenylenediamine showed the expected mutagenic effect, as was seen by a biologically relevant increase in mutant colonies compared to the corresponding negative controls.
Therefore, bis(2-ethylhexyl) hydrogen phosphate was considered negative without and with S9 mix in the plate incorporation as well as in the preincubation modification of the Salmonella/microsome test.
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