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Three acute fish studies were available. Two studies were performed with glycerine carbonate (Auferheide and Henkel) and one study was performed using the read-across substance glycerol (DOW, 1978). No mortality were seen at the highest concentrations tested, i.e. 1040 mg/L and 3000 mg/L glycerine carbonate. The LC50 based on glycerol was 885 mg/L. Based on these results, glycerine carbonate can be classified as not harmful for fish.

The acute 48 -hour LC50 value for Polyol 80, used as a read-across substance for glycerine carbonate, to Daphnia magna was examined (DOW, 1878). Under the conditions of the study, the 48-hour LC50 was 1955 mg/L, indicating that glycerine carbonate is not harmful for aquatic invertebrates.

In an experiment (Bringman, 1978) with glycerol as test substance and Scenedesmus quadricauda as test species, no effects were seen at > 10000 mg/L, indicating that glycerine carbonate is not harmful for aquatic algae.

The toxicity threshold was determined for glycerol using Pseudomonas putida (Bringman, 1980). The toxicity threshold (comparable to a NOEC) in a 16 hour study was >10,000 mg./L. While this study does not follow the typical activated sludge respiration inhibition study design, it does demonstrate that glycerol is relatively non-toxic to bacteria.

Based on the above results, glycerine carbonate should not be classified for environmental effects.

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