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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

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Ecotoxicological information

Short-term toxicity to fish

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Administrative data

Link to relevant study record(s)

Description of key information

96h LC50 > 100 mg/l for all species investigated. Rainbow trout study considered key, when expressed as HEDTA-FeNa, this would be > 96.2 mg/L 

Key value for chemical safety assessment

Additional information

Data are available for three short-term studies on fish:

The assessment of the 96 h LC50 of FeNaEDTA in fish was done in an acute test on the rainbow trout Onchorhynchus mykiss. No mortalities of fish occurred up to a concentration of 100 mg/L within a period of 96 h. The mean measured concentration of the test item was between 80 and 120% of the nominal through the entire test period and the toxicological endpoints were evaluated using nominal concentration of the test item. The LC50 (96h) was determined to be > 100 mg/L with a probability of 99.9%. The NOEC (96 h) was determined to be 100 mg/L.

The acute toxicity (96h-LC50) of sodium ferric EDTA towards Pimephales promelas was investigated in a multispecies test. The toxicity value was found to be >100 mg/l.


A third study with DTPA-FeNaH confirmed low toxicity to zebra fish (Danio rerio). The 96h LC50 value was > 100 mg/L.

All studies were found to be of good quality and were comparable to or equivalent to guideline studies.