Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Currently viewing:

Administrative data

Link to relevant study record(s)

Description of key information

48h EC50 100.9 mg/l EDTA-FeNa for Daphnia magna, corresponding to 97 mg/L HEDTA-FeNa

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
97 mg/L

Additional information

Four studies report on the toxicity of the test substance to Daphnia magna.

1. The toxicity of FeNaEDTA on Daphnia magna was tested in a 48 h static immobilisation test (Borrmann 2007). Test concentrations were measured and were within the range of 80% of the nominal concentration. The EC50 (mobility) was 100.9 mg/L (nominal) and the NOEC was 30 mg/L (reliability 1, Key study).

2. The acute toxicity (96h-LC50) of sodium ferric EDTA towards Daphnia magna was investigated in a multispecies test system (published by Ewell et al., 1986). The 96h-LC50 was 32 mg/l (nominal; published, reliability 2).

3. The acute toxicity of EDTA, Mn(II)+EDTA and Fe(III)+EDTA towards Daphnia magna was investigated (published by Sorvari and Sillanpaa 1996, Reliability 2). The 24h-EC50 for Fe(III)+EDTA expressed as Fe(III) (= 17 mg/l) equals the value for Fe(III) when tested without EDTA (16 mg/l).

The 24h-EC50 for Fe(III)+EDTA expressed as EDTA is 89.3 mg/l. This is a lower value then the 610 mg/l for EDTA on its own. This may be due to the presence of free Fe present in the EDTA-Fe mixture that is absent in the test with EDTA on its own. The 24h-EC50 expressed as the test substance is 128.3 mg/l.

4. In addition, an acute study with DTPA-FeNaH was performed in which low toxicity to daphnids was confirmed (Kean, 2013). The 48 h EC50 value was > 82.1 mg/L (measured conc).

The key study with 48h-EC50 = 100.9 mg/l was selected because this was the most relevant study providing a concrete EC50 and was carried out according to the guidelines and under GLP. The study by Kean gave a > value only. The other two studies were either longer (96h) or shorter and had other shortcomings and gaps of information.