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REACH

Methyl 2-naphthyl ether

EC number: 202-213-6 | CAS number: 93-04-9

Life Cycle description
Uses advised against

Ecotoxicological information

Toxicity to terrestrial plants

Currently viewing:

Administrative data

Link to relevant study record(s)

Reference

Endpoint:

toxicity to terrestrial plants: long-term

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 208 (Terrestrial Plants Test: Seedling Emergence and Seedling Growth Test)

GLP compliance:

yes

Analytical monitoring:

yes

Details on sampling:

- Concentrations: Low mid and high concentration was analysed for quantity of test chemical in exposed concentrations.
Range of average analyzed content of active ingredient (% of nominal)
Plant species Test conc. % recovery
Onion 15, 135 and 1215 81.630 - 93.599
Tomato 5, 45 and 405 92.714 - 96.095
Corn and cabbage 32, 200 and 1250 106.323 - 114.655
Soyabean and sunflower 15, 135 and 1215 98.344 - 106.382

- Sampling method: . Three replicates from low, mid and high dose group, and two replicates from control and vehicle control group samples were processed by transferring 5 grams of test sample in 50 ml of conical flask and 10 ml acetone. and the process was continued by sonicating for 2 minutes. Then the sample were transferred to 15 mL Tarson tube and centrifuged (3000 rpm, at 25°C, 10 min). The supernatant was diluted with acetone, to obtain the final concentration of approximately 0.004 µg/mL of test item and injected to GC-MS/MS.
- Sample storage conditions before analysis: sample was immediately asnalysed soon after sampling

Vehicle:

yes

Remarks:

acetone

Details on preparation and application of test substrate:

The artificial substrate was used as the test medium. Acid washed quartz sand (maximum size of 2 mm in diameter) was used as artificial substrate. Within a given phase of test, all the artificial substrate was from the same source.

Maximum Water Holding Capacity of the Soil
Defined quantity of the acid washed quartz sand was loaded into the Keen’s cup (with filter paper in inner chamber). The Keen’s cup was gradually submerged until the water level is above the top of sand. The Keen’s cup was left in the water for three hours. The sand sample was allowed to drain by placing the Keen’s cup onto a filter paper until water draining on to the filter paper was ceased. The Keen’s cup was then weighed followed by drying to constant mass at 105 ± 5 °C. The maximum water holding capacity (WHCmax) was calculated as 26%
- Controls: Artifical soil
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): acetone
- Concentration of vehicle in test medium (stock solution and final test solution): 10 ml of acetone in 2.3kg of substrate
- Evaporation of vehicle before use: yes

Species:

Allium cepa

Plant group:

Monocotyledonae (monocots)

Details on test organisms:

- Common name: onion
- Plant family: Amaryllidaceae
- Source of seed: Vokkal Seeds Pvt. Ltd

Species:

Zea mays

Plant group:

Monocotyledonae (monocots)

Details on test organisms:

- Common name: corn
- Plant family: Poaceae (Gramineae)
- Source of seed: Vokkal Seeds Pvt. Ltd.

Species:

Helianthus annuus

Plant group:

Monocotyledonae (monocots)

Details on test organisms:

- Common name: Sunflower
- Plant family: Asteraceae (Compositae)
- Source of seed: Vokkal Seeds Pvt. Ltd.

Species:

Brassica oleracea var. capitata

Plant group:

Dicotyledonae (dicots)

Details on test organisms:

- Common name: cabbage
- Plant family: Brassicaceae (Cruciferae)
- Variety: capitata
- Source of seed: Vokkal Seeds Pvt. Ltd.

Species:

other: Solanum lycopersicon

Plant group:

Dicotyledonae (dicots)

Details on test organisms:

- Common name: Tomato
- Plant family: Solanaceae
- Source of seed: Vokkal Seeds Pvt. Ltd

Species:

Glycine max (G. soja)

Plant group:

Dicotyledonae (dicots)

Details on test organisms:

- Common name: soybean
- Plant family: Fabaceae (Leguminosae
- Source of seed: Vokkal Seeds Pvt. Ltd.

Test type:

seedling emergence and seedling growth test

Study type:

laboratory study

Substrate type:

artificial soil

Limit test:

Total exposure duration:

21 d

Test temperature:

Onion Tomato: Air temperature range was 19.7 to 22.4 °C
Corn and Cabbage: Air temperature range was 19.6 to 23.0 °C
Soybean and Sunflower: Air temperature range was 19.5 to 23.3 °C

pH:

5.51

Moisture:

26% moisture

Details on test conditions:

TEST SYSTEM
- Testing facility: Laboratory
- Test container (type, material, size):non porous plastic pots, with diameter: 15.5 cm, surface area about: 189 cm2
- Amount of soil: 2kg/pot
- No. of seeds per container:
Plant species Plants per concentration Plants per pot Pots per concentration Planting depth (mm)
Onion 5 20 4 5
Corn 2 20 10 10
Cabbage 3 21 7 5
Tomato 3 21 7 5
Sunflower 3 21 7 10
Soybean 3 21 7 10

- No. of plants (retained after thinning):
- No. of replicates per treatment group: 4 replicates for onion and 10 replicates for corn; 7 replicates for cabbage, tomato, sunflower and soybean
- No. of replicates per control: 4 replicates for onion and 10 replicates for corn; 7 replicates for cabbage, tomato, sunflower and soybean
- No. of replicates per vehicle control: 4 replicates for onion and 10 replicates for corn; 7 replicates for cabbage, tomato, sunflower and soybean
GROWTH CONDITIONS
- Photoperiod: controlled light-dark cycles with 16 hours light and 8 hours dark
- Light source: PAR light (photosynthetically active radiation) wavelength 400 - 700 nm
- Light intensity and quality: between 22600 to 24100 Lux.
- Relative humidity (%): 71 to 89 %.
-Carbondioxide: 340 to 370 ppm
- Watering regime and schedules: Initial top watering was used to stimulate seed germination. After seedling emergence, bottom and top watering (~ 40 to 50 mL/pot sprinkled over artificial substrate and tray) were used. Frequency of watering as well as volume of water used for watering was dependent on artificial substrate moisture content. At any point during watering, water logging situations was avoided

VEHICLE CONTROL PERFORMED: yes

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 3
- Justification for using less concentrations than requested by guideline: test concentrations were used as per test guidelines
- Range finding study
Test concentrations of 10, 100, 500 and 1000 mg/kg dry weight of artificial substrate was tested along with vehicle control during the range finding test.
- Results used to determine the conditions for the definitive study: : growth and development of plant

Nominal and measured concentrations:

Nominal concentrations

Reference substance (positive control):

yes

Species:

Allium cepa

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

45 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

seedling emergence

Species:

Allium cepa

Duration:

21 d

Dose descriptor:

LOEC

Effect conc.:

135 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

seedling emergence

Species:

Allium cepa

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

405 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: Seed survival

Species:

Allium cepa

Duration:

21 d

Dose descriptor:

LOEC

Effect conc.:

> 405 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: Seedling Survival

Species:

Allium cepa

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

45 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth

Species:

Allium cepa

Duration:

21 d

Dose descriptor:

LOEC

Effect conc.:

135 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth

Species:

Allium cepa

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

45 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: shoot weight

Species:

Allium cepa

Duration:

21 d

Dose descriptor:

LOEC

Effect conc.:

135 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: Dry shoot weight

Species:

Zea mays

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

200 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

seedling emergence

Species:

Zea mays

Duration:

21 d

Dose descriptor:

LOEC

Effect conc.:

500 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

seedling emergence

Species:

Zea mays

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

500 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: seedling survival

Species:

Zea mays

Duration:

21 d

Dose descriptor:

LOEC

Effect conc.:

1 250 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: seedling survival

Species:

Zea mays

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

80 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth

Species:

Zea mays

Duration:

21 d

Dose descriptor:

LOEC

Effect conc.:

200 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth

Species:

Zea mays

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

200 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: Dry shoot weight

Species:

Zea mays

Duration:

21 d

Dose descriptor:

LOEC

Effect conc.:

500 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: Dry shoot weight

Species:

Brassica oleracea var. capitata

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

200 ng/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

seedling emergence

Species:

Brassica oleracea var. capitata

Duration:

21 d

Dose descriptor:

LOEC

Effect conc.:

500 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

seedling emergence

Species:

Brassica oleracea var. capitata

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

1 250 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: seedling survival

Species:

Brassica oleracea var. capitata

Duration:

21 d

Dose descriptor:

LOEC

Effect conc.:

> 1 250 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: seedling survival

Species:

Brassica oleracea var. capitata

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

32 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth

Species:

Brassica oleracea var. capitata

Duration:

21 d

Dose descriptor:

LOEC

Effect conc.:

80 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth

Species:

Brassica oleracea var. capitata

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

80 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: Dry shoot weight

Species:

Brassica oleracea var. capitata

Duration:

21 d

Dose descriptor:

LOEC

Effect conc.:

200 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: Dry shoot weight

Species:

other: Solanum lycopersicon

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

5 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

seedling emergence

Species:

other: Solanum lycopersicon

Duration:

21 d

Dose descriptor:

LOEC

Effect conc.:

15 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

seedling emergence

Species:

other: Solanum lycopersicon

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

405 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: seedling survival

Species:

other: Solanum lycopersicon

Duration:

21 d

Dose descriptor:

LOEC

Effect conc.:

> 405 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: seedling survival

Species:

other: Solanum lycopersicon

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

15 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth

Species:

other: Solanum lycopersicon

Duration:

21 d

Dose descriptor:

LOEC

Effect conc.:

45 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth

Species:

other: Solanum lycopersicon

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

15 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: dry shoot weight

Species:

other: Solanum lycopersicon

Duration:

21 d

Dose descriptor:

LOEC

Effect conc.:

45 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: dry shoot weight

Species:

Glycine max (G. soja)

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

135 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

seedling emergence

Species:

Glycine max (G. soja)

Duration:

21 d

Dose descriptor:

LOEC

Effect conc.:

405 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

seedling emergence

Species:

Glycine max (G. soja)

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

405 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: seedling survival

Species:

Glycine max (G. soja)

Duration:

21 d

Dose descriptor:

LOEC

Effect conc.:

1 215 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: seedling survival

Species:

Glycine max (G. soja)

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

15 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth

Species:

Glycine max (G. soja)

Duration:

21 d

Dose descriptor:

LOEC

Effect conc.:

45 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth

Species:

Glycine max (G. soja)

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

135 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: Dry shoot weight

Species:

Glycine max (G. soja)

Duration:

21 d

Dose descriptor:

LOEC

Effect conc.:

405 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: Dry shoot weight

Species:

Helianthus annuus

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

405 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

seedling emergence

Species:

Helianthus annuus

Duration:

21 d

Dose descriptor:

LOEC

Effect conc.:

1 215 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

seedling emergence

Species:

Helianthus annuus

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

405 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: seedling survival

Species:

Helianthus annuus

Duration:

21 d

Dose descriptor:

LOEC

Effect conc.:

1 215 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: seedling survival

Species:

Helianthus annuus

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

15 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth

Species:

Helianthus annuus

Duration:

21 d

Dose descriptor:

LOEC

Effect conc.:

45 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth

Species:

Helianthus annuus

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

15 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: Dry shoot weight

Species:

Helianthus annuus

Duration:

21 d

Dose descriptor:

LOEC

Effect conc.:

45 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: dry shoot weight

Details on results:

SEED GERMINATION in control
- Percent seed germination: 100

SEEDLING EMERGENCE
- Percent seedling emergence: 90
- Percent survival: 100

Reported statistics and error estimates:

A dose-response relationship was established in terms of a regression equation by using four parameter Model. Different model was used for estimating ECx (EC10, EC20, EC50, EC90) and its confidence limits, where possible.
The statistical analysis of the shoot height and shoot weight data was evaluated using licensed copies of SYSTAT Statistical package version 12.0. Data was tested for normality (Shapiro-Wilk test) and homogeneity of variance (Levene’s) before performing further analysis. When data was found as normal and homogeneous, ANOVA was performed, when data was found as non-normal or non-homogeneous, data were transformed, and ANOVA was done on transformed data for overall group comparison. Dunnett’s test was performed for pairwise comparison. Comparison of two group’s mean was done using two-sample t-test.
All analyses and comparisons were evaluated at the 5% (p<0.05) level. The unit of expression of endpoints was mg/kg dry weight of artificial substrate.

Range finding test:

Plant species: Onion
Test conc. (mg/kg)	% of Seedling emergence		% of Seedling survival		% Shoot height reduction		% Dry shoot weight reduction
0 (V)	80.0		100.0		-		-
10	80.0		100.0		2.0		-0.2
100	80.0		100.0		2.0		-0.7
500	50.0		100.0		-45.2		-46.8
1000	30.0		100.0		-50.7		-56.0
Plant species:Corn
0 (V)	75.0		100.0		-		-
10	75.0		100.0		3.3		1.8
100	87.5		100.0		0.8		-1.4
500	62.5		100.0		-53.2		-52.9
1000	50.0		100.0		-78.6		-69.8
Plant species: Cabbage
Test conc. (mg/kg)		% of Seedling emergence		% of Seedling survival		% Shoot height reduction	% Dry shoot weight reduction
0 (V)		77.8		100.0		-	-
10		77.8		100.0		0.4	1.0
100		100.0		100.0		-0.9	-1.7
500		11.1		100.0		-68.1	-45.4
1000		11.1		100.0		-70.2	-49.0
Plant species:Tomato
0 (V)		100.0		100.0		-	-
10		100.0		100.0		3.9	1.4
100		55.6		100.0		-24.0	-51.6
500		11.1		100.0		-75.1	-79.8
1000		0.0		-		-	-
Plant species: Soybean
0 (V)		100.0		100.0		-	-
10		100.0		100.0		1.3	0.1
100		100.0		100.0		1.5	-2.7
500		55.6		55.6		-75.7	-37.5
1000		22.2		22.2		-79.0	-56.1
Plant species: Sunflower
0 (V)		100.0		100.0		-	-
10		100.0		100.0		1.4	0.3
100		100.0		100.0		1.6	-1.2
500		88.9		88.9		-64.7	-49.1
1000		44.4		44.4		-85.3	-59.2

Definitive test

Plant species: Onion
Test conc. (mg/kg)	% of Seedling emergence	% of Seedling survival	% Shoot height reduction	% Dry shoot weight reduction
0	90.0	100.0	-	-
0	90.0	100.0	-	-
15	90.0	100.0	0.9	3.1
45	100.0	100.0	-19.4	-20.9
135	80.0	100.0	*-58.7	*-56.7
405	55.0	100.0	*-89.1	*-78.0
1215	0.0	NA	NA	NA
Plant species:Corn
0	90.0	100.0	-	-
0	90.0	100.0	-	-
32	90.0	100.0	0.3	-0.2
80	90.0	100.0	-13.3	-9.2
200	95.0	100.0	*-35.6	-28.8
500	75.0	100.0	*-46.8	*-44.7
1250	35.0	71.4	*-88.0	*-88.2
Plant species: Cabbage
0	90.5	100.0	-	-
0	90.5	100.0	-	-
32	100.0	100.0	-0.1	-0.5
80	95.2	100.0	*-12.7	-16.9
200	90.5	100.0	*-34.2	*-37.8
500	42.9	100.0	*-75.9	*-64.4
1250	19.0	100.0	*-85.9	*-82.3
Plant species:Tomato
0	95.2	100.0	-	-
0	95.2	100.0	-	-
5	95.2	100.0	0.3	-0.2
15	81.0	100.0	-17.3	-13.6
45	66.7	100.0	*-37.8	*-37.5
135	57.1	100.0	*-71.8	*-68.5
405	23.8	100.0	*-86.6	*-81.9
Plant species: Soybean
0	95.2	100.0	-	-
0	95.2	100.0	-	-
15	95.2	100.0	-1.7	0.4
45	100.0	100.0	*-10.8	-6.7
135	95.2	100.0	*-27.1	-24.1
405	90.5	100.0	*-56.8	*-55.4
1215	57.1	83.3	*-94.4	*-88.7

Plant species: Sunflower
Test conc. (mg/kg)	% of Seedling emergence	% of Seedling survival	% Shoot height reduction	% Dry shoot weight reduction
0	100.0	100.0	-	-
0	95.2	100.0	-	-
15	100.0	100.0	2.9	-1.8
45	95.2	100.0	*-33.4	*-30.0
135	95.2	100.0	*-41.6	*-39.3
405	95.2	100.0	*-70.6	*-67.5
1215	23.8	40.0	*-93.8	*-88.0

Test Item Active Ingredient in Test Medium

Active ingredient	Range of average analyzed content of active ingredient (% of nominal)
Active ingredient	Plant species	Test conc.	Day 0
test item	Onion	15, 135 and 1215	81.630 - 93.599
	Tomato	5, 45 and 405	92.714 - 96.095
	Corn and cabbage	32, 200 and 1250	106.323 - 114.655
	Soyabean and sunflower	15, 135 and 1215	98.344 - 106.382

TABLE 1. Definitive[c1] [r2] Test Conditions - Air Temperature and Relative Humidity

Plant species: Onion and Tomato

Day	Air temperature(°C)		Relative humidity (%)
Day	Minimum	Maximum	Minimum	Maximum
0	19.8	22.0	71	86
1	19.9	22.1	73	88
2	19.8	22.3	72	87
3	19.9	22.4	74	89
4	19.8	22.3	73	87
5	19.7	22.2	72	86
6	19.8	22.1	73	88
7	19.9	22.3	74	87
8	19.8	22.1	73	86
9	19.9	22.2	74	88
10	20.0	22.2	73	87
11	19.9	22.1	74	86
12	20.0	22.1	73	88
13	19.9	22.3	72	87
14	19.8	22.2	71	86
15	19.9	22.1	73	87
16	19.9	22.2	72	89
17	19.8	22.0	73	86
18	19.9	22.1	74	85
19	20.0	22.2	73	84
20	19.9	22.3	74	86
21	20.0	22.2	72	85

Air temperature range was 19.7 to 22.4 °C and relative humidity range was 71 to 89 %.

Plant species: Corn and Cabbage

Day	Air temperature(°C)		Relative humidity (%)
Day	Minimum	Maximum	Minimum	Maximum
0	19.9	23.0	73	84
1	19.8	22.1	76	85
2	19.6	22.9	75	83
3	19.7	22.5	74	81
4	19.6	22.3	76	83
5	19.7	22.2	73	82
6	19.8	22.7	74	83
7	19.6	22.0	75	84
8	19.7	22.3	76	86
9	19.8	22.5	73	89
10	19.6	22.9	75	85
11	19.7	22.2	74	84
12	19.6	22.9	76	81
13	19.8	22.6	73	86
14	19.7	22.6	73	84
15	19.9	22.7	72	83
16	19.6	22.8	75	81
17	19.8	22.9	75	83
18	19.6	22.7	73	81
19	19.8	22.2	75	84

Air temperature range was 19.6 to 23.0 °C and relative humidity range was 72 to 89 %.

Plant species: Soybean and Sunflower

Day	Air temperature(°C)		Relative humidity (%)
Day	Minimum	Maximum	Minimum	Maximum
0	19.9	23.3	76	85
1	19.6	22.3	74	84
2	19.8	22.5	76	85
3	19.7	22.6	75	86
4	19.6	22.4	73	85
5	19.7	22.3	74	84
6	19.8	22.5	73	83
7	19.5	22.3	74	85
8	19.7	22.6	75	86
9	19.6	22.5	76	83
10	19.6	22.6	73	84
11	19.5	22.4	75	86
12	19.7	22.1	76	84
13	19.8	22.3	73	85
14	19.6	22.7	72	84
15	19.5	22.7	75	86
16	19.7	22.7	79	84
17	19.9	22.9	74	85
18	19.7	22.8	72	83
19	19.6	22.3	71	82

Air temperature range was 19.5 to 23.3 °C and relative humidity range was 71 to 86 %.

TABLE 1. Definitive Test Conditions - Carbon Dioxide and Light Intensity[c1] [r2]

Plant species: Onion and Tomato

Day	Carbon dioxide in air (ppm)	Light intensity (Lux) range
0	340	22700	23400	22900	23600
1	350	22800	23600	23100	23800
2	350	22600	23500	23200	23600
3	350	23100	23600	22900	23400
4	350	23300	22700	23100	22700
5	350	22900	22800	23200	23100
6	350	22700	23100	22800	23200
7	350	22800	23100	23000	22900
8	350	22900	23000	23100	22800
9	350	22900	22800	23100	23200
10	350	22900	23000	23100	23000
11	350	22800	22900	23000	22900
12	350	22900	23100	23200	23000
13	350	22800	22900	23100	23000
14	350	22900	23000	23100	23000
15	350	22900	22800	23100	23000
16	350	23100	23000	22800	23200
17	350	23000	22900	22800	23100
18	350	22900	22800	23000	23100
19	350	22800	22900	23100	23000
20	350	22900	22900	23200	23100
21	350	22800	22900	23100	23000

Carbon dioxide in air range was 340 to 350 ppm and light intensity range was 22600 to 23800 Lux.

Plant species: Corn and Cabbage

Day	Carbon dioxide in air (ppm)	Light intensity (Lux) range
0	370	23700	23900	23800	24000
1	360	23500	23700	23900	24100
2	350	22900	23100	23700	24000
3	350	22900	23200	23100	23000
4	350	23000	23100	23100	23200
5	350	23100	23000	23100	23300
6	350	23000	23100	23200	22900
7	350	23000	23200	23100	23300
8	350	23400	23200	23100	23300
9	350	23100	23300	23400	23200
10	350	23400	23100	23600	23500
11	360	23100	23000	23100	23300
12	350	23600	23500	23200	23600
13	350	23500	23600	23500	23100
14	350	23100	23000	22900	23000
15	350	23600	23500	23100	23400
16	350	23200	23200	23500	23100
17	350	23200	23100	23300	23400
18	350	24000	23800	23900	23700
19	350	23700	23400	23600	23500

Carbon dioxide in air range was 350 to 370 ppm and light intensity range was 22900 to 24100 Lux.

Plant species: Soybean and Sunflower

Day	Carbon dioxide in air (ppm)	Light intensity (Lux) range
0	340	23100	23400	23200	23700
1	360	23400	22900	23100	23600
2	370	23500	22700	23200	24100
3	350	23200	23100	23300	23100
4	350	23300	23100	23200	23200
5	360	23100	23300	23400	23200
6	350	23500	23300	23700	23400
7	350	23100	23600	23500	23000
8	360	23000	23100	23600	23200
9	350	23400	22900	23000	23200
10	350	23100	23500	23600	23400
11	360	23100	23200	23400	23100
12	350	23200	23600	23400	23200
13	350	23600	23000	23200	23900
14	350	23400	23200	23600	23100
15	350	23700	23500	23600	23300
16	360	23500	23600	23500	23700
17	350	23300	23200	23000	23400
18	350	23400	23200	22900	23100
19	350	23500	23100	23300	23200

Carbon dioxide in air range was 340 to 370 ppm and light intensity range was 22700 to 24100 Lux.

TABLE 1. Onion DT - Seedling Emergence, Seedling Survival, Shoot Height, and Dry Shoot Weight[c1] [r2]

Group & Replicate	Total no. of seeds emerged	% of Seedling emergence	Seedlings survival on day 14	Total no. of seedlings survival on day 14	% of Seedling survival	8-Aug	9-Aug	10-Aug	11-Aug	12-Aug	13-Aug	14-Aug	Seedling emergence		Plant observations
													*Day 7	*Day 14	*Day 7	*Day 14
													21-Aug	28-Aug	21-Aug	28-Aug
CR1	4	90.0	4	18	100.0	0	0	0	0	0	1	4	4	4	N(4), 0%(4)	N(4), 0%(4)
CR2	5		5			0	0	0	0	0	2	5	5	5	N(5), 0%(5)	N(5), 0%(5)
CR3	5		5			0	0	0	0	0	1	5	5	5	N(5), 0%(5)	N(5), 0%(5)
CR4	4		4			0	0	0	0	0	1	4	4	4	N(4), 0%(4)	N(4), 0%(4)
VR1	5	90.0	5	18	100.0	0	0	0	0	0	1	5	5	5	N(5), 0%(5)	N(5), 0%(5)
VR2	5		5			0	0	0	0	0	1	5	5	5	N(5), 0%(5)	N(5), 0%(5)
VR3	4		4			0	0	0	0	0	0	4	4	4	N(4), 0%(4)	N(4), 0%(4)
VR4	4		4			0	0	0	0	0	0	4	4	4	N(4), 0%(4)	N(4), 0%(4)
T1R1	5	90.0	5	18	100.0	0	0	0	0	0	0	5	5	5	N(5), 0%(5)	N(5), 0%(5)
T1R2	5		5			0	0	0	0	0	1	5	5	5	N(5), 0%(5)	N(5), 0%(5)
T1R3	4		4			0	0	0	0	0	1	4	4	4	N(4), 0%(4)	N(4), 0%(4)
T1R4[YB3]	4		4			0	0	0	0	0	1	4	4	4	N(4), 0%(4)	N(4), 0%(4)
T2R1	5	100.0	5	20	100.0	0	0	0	0	0	0	5	5	5	S(5), 40%(5)	S(5), 40%(5)
T2R2	5		5			0	0	0	0	0	0	5	5	5	N(5), 0%(5)	N(5), 0%(5)
T2R3	5		5			0	0	0	0	0	0	5	5	5	S(5), 25%(5)	S(5), 25%(5)
T2R4	5		5			0	0	0	0	0	1	5	5	5	N(5), 0%(5)	N(5), 0%(5)
T3R1	3	80.0	3	16	100.0	0	0	0	0	0	0	3	3	3	S(3), 50%(3)	S(3), 50%(3)
T3R2	5		5			0	0	0	0	0	0	5	5	5	S(5), 50%(5)	S(5), 50%(5)
T3R3	4		4			0	0	0	0	0	1	4	4	4	S(4), 60%(3), 90%(1)	S(4), 60%(3), 90%(1)
T3R4	4		4			0	0	0	0	0	0	4	4	4	S(4), 70%(4)	S(4), 70%(4)
T4R1	4	55.0	4	11	100.0	0	0	0	0	0	0	4	4	4	S(4), 90%(4)	S(4), 90%(4)
T4R2	3		3			0	0	0	0	0	0	3	3	3	S(3), 90%(3)	S(3), 90%(3)
T4R3	2		2			0	0	0	0	0	0	2	2	2	S(2), 90%(2)	S(2), 90%(2)
T4R4	2		2			0	0	0	0	0	0	2	2	2	S(2), 90%(2)	S(2), 90%(2)
T5R1	0	0.0	-	-	-	0	0	0	0	0	0	0	0	0	-	-
T5R2	0		-			0	0	0	0	0	0	0	0	0	-	-
T5R3	0		-			0	0	0	0	0	0	0	0	0	-	-
T5R4	0		-			0	0	0	0	0	0	0	0	0	-	-

Note: *: 7 and 14 days after 50% emergence of the seedlings in the control(s) group; N: Normal, S:Stunted growth.

Onion DT - Seedling Emergence, Seedling Survival, Shoot Height, and Dry Shoot Weight[c1] [r2]

Group & Replicate	Shoot height on day 14						Dry shoot weight (g) on day 14	Dry shoot weight (mg) on day 14	% Shoot height reduction on day 14	Group dry shoot weight (g) on day 14	Average dry shoot weight (g) on day 14	% Dry shoot weight reduction on day 14
	1	2	3	4	5	Average	Dry shoot weight (g) on day 14
	28-Aug						29-Aug
CR1	8.5	11.0	9.7	9.8	-	9.8	0.0251	25.1	-	0.1164	0.0065	-
CR2	8.8	11.2	9.6	10.4	9.6	9.9	0.0318	31.8
CR3	9.3	10.6	11.2	8.6	11.3	10.2	0.0332	33.2
CR4	9.8	9.6	8.8	11.4	-	9.9	0.0263	26.3
VR1	10.3	9.8	9.5	8.6	9.6	9.6	0.0312	31.2	-	0.1176	0.0065	-
VR2	10.6	10.3	11.3	9.3	10.7	10.4	0.0329	32.9
VR3	9.9	11.0	10.4	10.8	-	10.5	0.0274	27.4
VR4	8.8	10.1	8.7	10.3	-	9.5	0.0261	26.1
T1R1	10.4	9.6	10.1	10.3	10.6	10.2	0.0327	32.7	0.9[YB3]	0.1212	0.0067	3.1
T1R2	10.7	11.0	8.6	11.3	11.2	10.6	0.0338	33.8
T1R3	9.6	9.5	9.8	10.4	-	9.8	0.0276	27.6
T1R4	10.2	10.4	8.9	9.6	-	9.8	0.0271	27.1
T2R1	5.8	5.5	6.0	5.9	5.4	5.7	0.0183	18.3	-19.4	0.1034	0.0052	-20.9
T2R2	10.3	11.0	9.8	8.4	9.2	9.7	0.0314	31.4
T2R3	7.2	7.3	7.7	7.1	6.8	7.2	0.0238	23.8
T2R4	11.0	9.1	8.6	10.2	9.0	9.6	0.0299	29.9
T3R1	5.5	4.8	5.0	-	-	5.1	0.0096	9.6	-58.7*	0.0453	0.0028	-56.7*
T3R2	4.6	4.9	5.1	5.0	5.4	5.0	0.0164	16.4
T3R3	1.5	4.0	4.3	3.8	-	3.4	0.0104	10.4
T3R4	3.0	2.8	3.3	3.0	-	3.0	0.0089	8.9
T4R1	1.2	0.9	1.1	1.4	-	1.2	0.0064	6.4	-89.1*	0.0158	0.0014	-78.0*
T4R2	1.0	1.2	0.8	-	-	1.0	0.0043	4.3
T4R3	1.4	0.9	-	-	-	1.2	0.0029	2.9
T4R4	0.8	1.3	-	-	-	1.1	0.0022	2.2
T5R1	-	-	-	-	-	-	-	-	-	-	-	-
T5R2	-	-	-	-	-	-	-	-
T5R3	-	-	-	-	-	-	-	-
T5R4	-	-	-	-	-	-	-	-

*: Statistically significant difference in shoot height and dry shoot weight as compared with the vehicle control.

TABLE 1. Corn DT - Seedling Emergence, Seedling Survival, Shoot Height, and Dry Shoot Weight[c1] [r2]

Group & Replicate	Total no. of seeds emerged	% of Seedling emergence	Seedlings survival on day 14	Total no. of seedlings survival on day 14	% of Seedling survival	Seedling emergence					Seedling emergence		Plant observations
						Seedling emergence					*Day 7	*Day 14	*Day 7	*Day 14
						18-Sep	19-Sep	20-Sep	21-Sep	22-Sep	29-Sep	06-Oct	29-Sep	06-Oct
CR1	2	90.0	2	18	100.0	0	0	0	0	2	2	2	N(2), 0%(2)	N(2), 0%(2)
CR2	2		2			0	0	0	0	2	2	2	N(2), 0%(2)	N(2), 0%(2)
CR3	2		2			0	0	0	0	2	2	2	N(2), 0%(2)	N(2), 0%(2)
CR4	2		2			0	0	0	0	2	2	2	N(2), 0%(2)	N(2), 0%(2)
CR5	2		2			0	0	0	0	2	2	2	N(2), 0%(2)	N(2), 0%(2)
CR6	2		2			0	0	0	0	2	2	2	N(2), 0%(2)	N(2), 0%(2)
CR7	1		1			0	0	0	0	1	1	1	N(1), 0%(1)	N(1), 0%(1)
CR8	1		1			0	0	0	0	1	1	1	N(1), 0%(1)	N(1), 0%(1)
CR9	2		2			0	0	0	0	2	2	2	N(2), 0%(2)	N(2), 0%(2)
CR10	2		2			0	0	0	0	2	2	2	N(2), 0%(2)	N(2), 0%(2)
VR1	1	90.0	1	18	100.0	0	0	0	0	1	1	1	N(1), 0%(1)	N(1), 0%(1)
VR2	1		1			0	0	0	0	1	1	1	N(1), 0%(1)	N(1), 0%(1)
VR3	2		2			0	0	0	0	2	2	2	N(2), 0%(2)	N(2), 0%(2)
VR4	2		2			0	0	0	0	2	2	2	N(2), 0%(2)	N(2), 0%(2)
VR5	2		2			0	0	0	0	2	2	2	N(2), 0%(2)	N(2), 0%(2)
VR6	2		2			0	0	0	0	2	2	2	N(2), 0%(2)	N(2), 0%(2)
VR7	2		2			0	0	0	1	2	2	2	N(2), 0%(2)	N(2), 0%(2)
VR8	2		2			0	0	0	0	2	2	2	N(2), 0%(2)	N(2), 0%(2)
VR9	2		2			0	0	0	0	2	2	2	N(2), 0%(2)	N(2), 0%(2)
VR10	2		2			0	0	0	0	2	2	2	N(2), 0%(2)	N(2), 0%(2)

Note: *: 7 and 14 days after 50% emergence of the seedlings in the control(s) group; N: Normal.

Corn DT - Seedling Emergence, Seedling Survival, Shoot Height, and Dry Shoot Weight[c1] [r2]

Group & Replicate	Shoot height on day 14			Shoot weight on day 14	Dry shoot weight (mg) on day 14	% Shoot height reduction on day 14	Dry shoot weight (g) on day 14	Average dry shoot weight (g) on day 14	% Dry shoot weight reduction on day 14
	1	2	Average	Shoot weight on day 14
	06-Oct			07-Oct
CR1	15.9	16.4	16.2	0.7811	781.1	-	6.6223	0.3679	-
CR2	15.0	15.4	15.2	0.7323	732.3
CR3	16.6	15.2	15.9	0.7531	753.1
CR4	14.6	14.4	14.5	0.7118	711.8
CR5	13.9	15.7	14.8	0.7362	736.2
CR6	13.0	12.9	13.0	0.7034	703.4
CR7	16.1	-	16.1	0.3711	371.1
CR8	15.2	-	15.2	0.3562	356.2
CR9	14.3	14.2	14.3	0.7339	733.9
CR10	13.8	16.1	15.0	0.7432	743.2
VR1	14.6	-	14.6	0.3666	366.6	-	6.6615	0.3701	-
VR2	15.9	-	15.9	0.3775	377.5
VR3	14.3	13.9	14.1	0.7372	737.2
VR4	15.9	15.4	15.7	0.7443	744.3
VR5	16.0	15.2	15.6	0.7556	755.6
VR6	14.8	14.6	14.7	0.7118	711.8
VR7	15.2	14.6	14.9	0.7231	723.1
VR8	13.7	14.8	14.3	0.7311	731.1
VR9	16.2	15.3	15.8	0.7465	746.5
VR10	14.5	15.1	14.8	0.7678	767.8

Corn DT - Seedling Emergence, Seedling Survival, Shoot Height, and Dry Shoot Weight[c1] [r2]

Group & Replicate	Total no. of seeds emerged	% of Seedling emergence	Seedlings survival on day 14	Total no. of seedlings survival on day 14	% of Seedling survival	18-Sep	19-Sep	20-Sep	21-Sep	22-Sep	Seedling emergence		Plant observations
											*Day 7	*Day 14	*Day 7	*Day 14
											29-Sep	06-Oct	29-Sep	06-Oct
T1R1	1	90.0	1	18	100.0	0	0	0	0	1	1	1	N(1), 0%(1)	N(1), 0%(1)
T1R2	2		2			0	0	0	0	2	2	2	N(2), 0%(2)	N(2), 0%(2)
T1R3	2		2			0	0	0	0	2	2	2	N(2), 0%(2)	N(2), 0%(2)
T1R4	2		2			0	0	0	0	2	2	2	N(2), 0%(2)	N(2), 0%(2)
T1R5	2		2			0	0	0	0	2	2	2	N(2), 0%(2)	N(2), 0%(2)
T1R6	2		2			0	0	0	0	2	2	2	N(2), 0%(2)	N(2), 0%(2)
T1R7	2		2			0	0	0	0	2	2	2	N(2), 0%(2)	N(2), 0%(2)
T1R8	2		2			0	0	0	0	2	2	2	N(2), 0%(2)	N(2), 0%(2)
T1R9	2		2			0	0	0	0	2	2	2	N(2), 0%(2)	N(2), 0%(2)
T1R10	1		1			0	0	0	0	1	1	1	N(1), 0%(1)	N(1), 0%(1)
T2R1	2	90.0	2	18	100.0	0	0	0	0	2	2	2	N(2), 0%(2)	N(2), 0%(2)
T2R2	2		2			0	0	0	0	2	2	2	N(2), 0%(2)	N(2), 0%(2)
T2R3	1		1			0	0	0	0	1	1	1	N(1), 0%(1)	N(1), 0%(1)
T2R4	2		2			0	0	0	0	2	2	2	S(2), 20%(2)	S(2), 20%(2)
T2R5	2		2			0	0	0	0	2	2	2	S(2), 20%(2)	S(2), 20%(2)
T2R6	1		1			0	0	0	0	1	1	1	S(1), 30%(1)	S(1), 30%(1)
T2R7	2		2			0	0	0	0	2	2	2	S(2), 60%(1), 30%(1)	S(2), 60%(1), 30%(1)
T2R8	2		2			0	0	0	0	2	2	2	N(2), 0%(2)	N(2), 0%(2)
T2R9	2		2			0	0	0	0	2	2	2	N(2), 0%(2)	N(2), 0%(2)
T2R10	2		2			0	0	0	0	2	2	2	N(1), S(1), 0%(1), 20%(1)	N(1), S(1), 0%(1), 20%(1)
T3R1	2	95.0	2	19	100.0	0	0	0	0	1	2	2	S(2), 20%(1), 50%(1)	S(2), 20%(1), 50%(1)
T3R2	2		2			0	0	0	0	2	2	2	S(2), 20%(1), 30%(1)	S(2), 20%(1), 30%(1)
T3R3	2		2			0	0	0	0	0	2	2	S(2), 30%(2)	S(2), 30%(2)
T3R4	2		2			0	0	0	0	0	2	2	S(2), 10%(1), 40%(1)	S(2), 10%(1), 40%(1)
T3R5	2		2			0	0	0	0	1	2	2	S(2), 20%(1), 50%(1)	S(2), 20%(1), 50%(1)
T3R6	2		2			0	0	0	0	1	2	2	S(2), 30%(1), 60%(1)	S(2), 30%(1), 60%(1)
T3R7	2		2			0	0	0	0	1	2	2	S(2), 30%(1), 50%(1)	S(2), 30%(1), 50%(1)
T3R8	2		2			0	0	0	0	0	2	2	S(2), 20%(1), 40%(1)	S(2), 20%(1), 40%(1)
T3R9	1		1			0	0	0	0	0	1	1	S(1), 60%(1)	S(1), 60%(1)
T3R10	2		2			0	0	0	0	0	2	2	S(2), 30%(1), 40%(1)	S(2), 30%(1), 40%(1)

Note: *: 7 and 14 days after 50% emergence of the seedlings in the control(s) group; N: Normal, S:Stunted growth.

contd. Corn DT - Seedling Emergence, Seedling Survival, Shoot Height, and Dry Shoot Weight[c1] [r2]

Group & Replicate	Shoot height on day 14			Shoot weight on day 14	Dry shoot weight (mg) on day 14	% Shoot height reduction on day 14	Dry shoot weight (g) on day 14	Average dry shoot weight (g) on day 14	% Dry shoot weight reduction on day 14
	1	2	Average	Shoot weight on day 14
	06-Oct			07-Oct
T1R1	13.9	-	13.9	0.3608	360.8	0.3[YB3]	6.6507	0.3695	-0.2
T1R2	14.2	14.8	14.5	0.7426	742.6
T1R3	15.3	13.7	14.5	0.7228	722.8
T1R4	16.0	13.9	15.0	0.7263	726.3
T1R5	15.8	14.8	15.3	0.7334	733.4
T1R6	14.7	15.5	15.1	0.7372	737.2
T1R7	15.3	15.8	15.6	0.7469	746.9
T1R8	16.2	15.3	15.8	0.7581	758.1
T1R9	15.3	14.9	15.1	0.7456	745.6
T1R10	16.0	-	16.0	0.3770	377.0
T2R1	13.2	14.0	13.6	0.6989	698.9	-13.3	6.0488	0.3360	-9.2
T2R2	15.2	14.5	14.9	0.7315	731.5
T2R3	15.0	-	15.0	0.3531	353.1
T2R4	11.6	12.2	11.9	0.6583	658.3
T2R5	10.7	12.0	11.4	0.6376	637.6
T2R6	11.0	-	11.0	0.2894	289.4
T2R7	11.1	6.4	8.8	0.4565	456.5
T2R8	16.2	16.4	16.3	0.7956	795.6
T2R9	14.5	15.4	15.0	0.7544	754.4
T2R10	13.9	11.1	12.5	0.6735	673.5
T3R1	12.2	7.3	9.8	0.5321	532.1	-35.6*	5.0094	0.2637	-28.8
T3R2	11.9	11.2	11.6	0.6401	640.1
T3R3	11.0	11.4	11.2	0.6119	611.9
T3R4	9.6	12.4	11.0	0.5993	599.3
T3R5	12.0	7.3	9.7	0.5282	528.2
T3R6	10.6	5.0	7.8	0.3892	389.2
T3R7	11.1	8.0	9.6	0.5126	512.6
T3R8	11.4	10.0	10.7	0.5578	557.8
T3R9	5.5	-	5.5	0.1165	116.5
T3R10	9.6	10.6	10.1	0.5217	521.7

*: Statistically significant difference in shoot height and dry shoot weight as compared with the vehicle control.

contd. Corn DT - Seedling Emergence, Seedling Survival, Shoot Height, and Dry Shoot Weight[c1] [r2]

Group & Replicate	Total no. of seeds emerged	% of Seedling emergence	Seedlings survival on day 14	Total no. of seedlings survival on day 14	% of Seedling survival	18-Sep	19-Sep	20-Sep	21-Sep	22-Sep	Seedling emergence		Plant observations
											*Day 7	*Day 14	*Day 7	*Day 14
											29-Sep	06-Oct	29-Sep	06-Oct
T4R1	2	75.0	2	15	100.0	0	0	0	1	1	2	2	S(2), 40%(1), 60%(1)	S(2), 40%(1), 60%(1)
T4R2	1		1			0	0	0	0	0	1	1	S(1), 80%(1)	S(1), 80%(1)
T4R3	1		1			0	0	0	0	0	1	1	S(1), 80%(1)	S(1), 80%(1)
T4R4	1		1			0	0	0	0	1	1	1	S(1), 40%(1)	S(1), 40%(1)
T4R5	2		2			0	0	0	0	2	2	2	S(2), 40%(1), 60%(1)	S(2), 40%(1), 60%(1)
T4R6	1		1			0	0	0	0	0	1	1	S(1), 40%(1)	S(1), 40%(1)
T4R7	2		2			0	0	0	0	1	2	2	S(2), 50%(2)	S(2), 50%(2)
T4R8	2		2			0	0	0	0	2	2	2	S(2), 40%(2)	S(2), 40%(2)
T4R9	2		2			0	0	0	0	1	2	2	S(2), 10%(1), 80%(1)	S(2), 10%(1), 80%(1)
T4R10	1		1			0	0	0	0	1	1	1	S(1), 40%(1)	S(1), 40%(1)
T5R1	2	35.0	0	5	71.4	0	0	0	0	0	2	2	S(2), 80%(2)	D(2), 100%(2)
T5R2	2		2			0	0	0	0	0	2	2	S(2), 90%(1), 80%(1)	S(2), 90%(1), 80%(1)
T5R3	1		1			0	0	0	0	0	1	1	S(1), 90%(1)	S(1), 90%(1)
T5R4	-		-			0	0	0	-	-	-	-	-	-
T5R5	-		-			0	0	0	-	-	-	-	-	-
T5R6	1		1			0	0	0	0	0	1	1	S(1), 80%(1)	S(1), 80%(1)
T5R7	1		1			0	0	0	0	0	1	1	S(1), 95%(1)	S(1), 95%(1)
T5R8	-		-			0	0	0	-	-	-	-	-	-
T5R9	-		-			0	0	0	-	-	-	-	-	-
T5R10	-		-			0	0	0	-	-	-	-	-	-

Note: *: 7 and 14 days after 50% emergence of the seedlings in the control(s) group; S:Stunted growth; D: Dead.

Corn DT - Seedling Emergence, Seedling Survival, Shoot Height, and Dry Shoot Weight[c1] [r2]

Group & Replicate	Shoot height on day 14			Shoot weight on day 14	Dry shoot weight (mg) on day 14	% Shoot height reduction on day 14	Dry shoot weight (g) on day 14	Average dry shoot weight (g) on day 14	% Dry shoot weight reduction on day 14
	1	2	Average	Shoot weight on day 14
	06-Oct			07-Oct
T4R1	10.0	7.0	8.5	0.4217	421.7	-46.8*	3.0692	0.2046	-44.7*
T4R2	2.0	-	2.0	0.0641	64.1
T4R3	2.4	-	2.4	0.0863	86.3
T4R4	11	-	11.0	0.2453	245.3
T4R5	7.0	11.0	9.0	0.4701	470.1
T4R6	9.9	-	9.9	0.2193	219.3
T4R7	8.3	8.5	8.4	0.3995	399.5
T4R8	11.0	9.4	10.2	0.4867	486.7
T4R9	13.0	2.0	7.5	0.3981	398.1
T4R10	11.0	-	11.0	0.2781	278.1
T5R1	-	-	-	-	-	-88.0*	0.2178	0.0436	-88.2*
T5R2	1.5	2.3	1.9	0.0932	93.2
T5R3	1.7	-	1.7	0.0381	38.1
T5R4	-	-	-	-	-
T5R5	-	-	-	-	-
T5R6	2.8	-	2.8	0.0632	63.2
T5R7	0.8	-	0.8	0.0233	23.3
T5R8	-	-	-	-	-
T5R9	-	-	-	-	-
T5R10	-	-	-	-	-

*: Statistically significant difference in shoot height and dry shoot weight as compared with the vehicle control.

TABLE 1. Cabbage DT - Seedling Emergence, Seedling Survival, Shoot Height, and Dry Shoot Weight[c1] [r2]

Group & Replicate	Total no. of seeds emerged	% of Seedling emergence	Seedlings survival on day 14	Total no. of seedlings survival on day 14	% of Seedling survival	18-Sep	19-Sep	20-Sep	21-Sep	Seedling emergence		Plant observations
										*Day 7	*Day 14	*Day 7	*Day 14
										28-Sep	05-Oct	28-Sep	05-Oct
CR1	2	90.5	2	19	100.0	0	0	0	2	2	2	N(2), 0%(2)	N(2), 0%(2)
CR2	3		3			0	0	0	1	3	3	N(3), 0%(3)	N(3), 0%(3)
CR3	3		3			0	0	0	2	3	3	N(3), 0%(3)	N(3), 0%(3)
CR4	3		3			0	0	0	3	3	3	N(3), 0%(3)	N(3), 0%(3)
CR5	3		3			0	0	0	3	3	3	N(3), 0%(3)	N(3), 0%(3)
CR6	3		3			0	0	0	3	3	3	N(3), 0%(3)	N(3), 0%(3)
CR7	2		2			0	0	0	2	2	2	N(2), 0%(2)	N(2), 0%(2)
VR1	1	90.5	1	19	100.0	0	0	0	1	1	1	N(1), 0%(1)	N(1), 0%(1)
VR2	3		3			0	0	0	3	3	3	N(3), 0%(3)	N(3), 0%(3)
VR3	3		3			0	0	0	3	3	3	N(3), 0%(3)	N(3), 0%(3)
VR4	3		3			0	0	1	3	3	3	N(3), 0%(3)	N(3), 0%(3)
VR5	3		3			0	0	1	3	3	3	N(3), 0%(3)	N(3), 0%(3)
VR6	3		3			0	0	1	3	3	3	N(3), 0%(3)	N(3), 0%(3)
VR7	3		3			0	0	0	3	3	3	N(3), 0%(3)	N(3), 0%(3)
T1R1	3	100.0	3	21	100.0	0	0	0	3	3	3	N(3), 0%(3)	N(3), 0%(3)
T1R2	3		3			0	0	0	3	3	3	N(3), 0%(3)	N(3), 0%(3)
T1R3	3		3			0	0	0	3	3	3	N(3), 0%(3)	N(3), 0%(3)
T1R4	3		3			0	0	0	3	3	3	N(3), 0%(3)	N(3), 0%(3)
T1R5	3		3			0	0	0	3	3	3	N(3), 0%(3)	N(3), 0%(3)
T1R6	3		3			0	0	0	3	3	3	N(3), 0%(3)	N(3), 0%(3)
T1R7	3		3			0	0	0	3	3	3	N(3), 0%(3)	N(3), 0%(3)
T2R1	3	95.2	3	20	100.0	0	0	0	1	3	3	S(3), 20%(3)	S(3), 20%(3)
T2R2	3		3			0	0	0	2	3	3	S(3), 30%(3)	S(3), 30%(3)
T2R3	3		3			0	0	0	3	3	3	N(3), 0%(3)	N(3), 0%(3)
T2R4	2		2			0	0	0	2	2	2	N(2), 0%(2)	N(2), 0%(2)
T2R5	3		3			0	0	0	3	3	3	N(3), 0%(3)	N(3), 0%(3)
T2R6	3		3			0	0	0	3	3	3	S(3), 10%(3)	S(3), 10%(3)
T2R7	3		3			0	0	0	3	3	3	S(3), 10%(3)	S(3), 10%(3)

Note: *: 7 and 14 days after 50% emergence of the seedlings in the control(s) group; N: Normal, S:Stunted growth.

contd. Cabbage DT - Seedling Emergence, Seedling Survival, Shoot Height, and Dry Shoot Weight[c1] [r2]

Group & Replicate	Shoot height on day 14				Dry shoot weight (g) on day 14	Dry shoot weight (mg) on day 14	% Shoot height reduction on day 14	Dry shoot weight (g) on day 14	Average dry shoot weight (g) on day 14	% Dry shoot weight reduction on day 14
	1	2	3	Average	Dry shoot weight (g) on day 14
	05-Oct				06-Oct
CR1	4.2	4.0	-	4.1	0.0888	88.8	-	0.8300	0.0437	-
CR2	4.3	4.7	4.2	4.4	0.1304	130.4
CR3	4.2	4.1	4.4	4.2	0.1222	122.2
CR4	4.1	4.2	4.6	4.3	0.1342	134.2
CR5	4.2	4.4	3.9	4.2	0.1265	126.5
CR6	3.8	4.9	4.5	4.4	0.1378	137.8
CR7	4.0	3.9	-	4.0	0.0901	90.1
VR1	4.7	-	-	4.7	0.0467	46.7	-	0.8363	0.0440	-
VR2	3.8	4.1	4.4	4.1	0.1236	123.6
VR3	4.2	3.9	4.8	4.3	0.1379	137.9
VR4	3.9	3.7	4.5	4.0	0.1194	119.4
VR5	4.8	4.8	4.9	4.8	0.1462	146.2
VR6	4.7	4.9	4.2	4.6	0.1335	133.5
VR7	4.2	4.0	3.9	4.0	0.1290	129.0
T1R1	3.8	4.8	4.3	4.3	0.1316	131.6	-0.1	0.9199	0.0438	-0.5
T1R2	4.2	4.6	3.9	4.2	0.1329	132.9
T1R3	4.4	3.9	4.8	4.4	0.1388	138.8
T1R4	4.7	4.0	4.9	4.5	0.1372	137.2
T1R5	3.9	4.7	3.8	4.1	0.1194	119.4
T1R6	4.2	4.6	4.9	4.6	0.1364	136.4
T1R7	4.2	4.9	4.2	4.4	0.1236	123.6
T2R1	3.0	3.8	3.9	3.6	0.1023	102.3	-12.7*	0.7317	0.0366	-16.9
T2R2	3.4	3.5	2.9	3.3	0.0973	97.3
T2R3	4.1	3.9	4.3	4.1	0.1227	122.7
T2R4	4.0	4.0	-	4.0	0.0822	82.2
T2R5	3.8	4.4	4.1	4.1	0.1193	119.3
T2R6	3.8	3.9	4.0	3.9	0.0983	98.3
T2R7	3.9	3.6	3.8	3.8	0.1096	109.6

*: Statistically significant difference in shoot height and dry shoot weight as compared with the vehicle control.

. Cabbage DT - Seedling Emergence, Seedling Survival, Shoot Height, and Dry Shoot Weight[c1] [r2]

Group & Replicate	Total no. of seeds emerged	% of Seedling emergence	Seedlings survival on day 14	Total no. of seedlings survival on day 14	% of Seedling survival	18-Sep	19-Sep	20-Sep	21-Sep	Seedling emergence		Plant observations
										*Day 7	*Day 14	*Day 7	*Day 14
										28-Sep	05-Oct	28-Sep	05-Oct
T3R1	3	90.5	3	19	100.0	0	0	0	2	3	3	S(3), 40%(3)	S(3), 40%(3)
T3R2	2		2			0	0	0	2	2	2	S(2), 40%(2)	S(2), 40%(2)
T3R3	2		2			0	0	0	2	2	2	S(2), 50%(2)	S(2), 50%(2)
T3R4	3		3			0	0	0	3	3	3	S(3), 20%(2), 50%(1)	S(3), 20%(2), 50%(1)
T3R5	3		3			0	0	0	2	3	3	S(3), 30%(3)	S(3), 30%(3)
T3R6	3		3			0	0	0	3	3	3	S(3), 30%(2), 60%(1)	S(3), 30%(2), 60%(1)
T3R7	3		3			0	0	0	3	3	3	S(3), 30%(3)	S(3), 30%(3)
T4R1	0	42.9	0	9	100.0	0	0	0	0	0	0	-	-
T4R2	0		0			0	0	0	0	0	0	-	-
T4R3	3		3			0	0	0	2	3	3	S(3), 70%(3)	S(3), 70%(3)
T4R4	3		3			0	0	0	0	3	3	S(3), 90%(3)	S(3), 90%(3)
T4R5	3		3			0	0	0	1	3	3	S(3), 80%(3)	S(3), 80%(3)
T4R6	0		0			0	0	0	0	0	0	-	-
T4R7	0		0			0	0	0	0	0	0	-	-
T5R1	0	19.0	0	4	100.0	0	0	0	0	0	0	-	-
T5R2	2		2			0	0	0	0	2	2	S(2), 95%(2)	S(2), 95%(2)
T5R3	0		0			0	0	0	0	0	0	-	-
T5R4	0		0			0	0	0	0	0	0	-	-
T5R5	1		1			0	0	0	0	1	1	S(1), 90%(1)	S(1), 90%(1)
T5R6	0		0			0	0	0	0	0	0	-	-
T5R7	1		1			0	0	0	0	1	1	S(1), 90%(1)	S(1), 90%(1)

Note: *: 7 and 14 days after 50% emergence of the seedlings in the control(s) group; S:Stunted growth.

Cabbage DT - Seedling Emergence, Seedling Survival, Shoot Height, and Dry Shoot Weight[c1] [r2]

Group & Replicate	Shoot height on day 14				Dry shoot weight (g) on day 14	Dry shoot weight (mg) on day 14	% Shoot height reduction on day 14	Dry shoot weight (g) on day 14	Average dry shoot weight (g) on day 14	% Dry shoot weight reduction on day 14
	1	2	3	Average	Dry shoot weight (g) on day 14
	05-Oct				06-Oct
T3R1	2.9	2.6	3.3	2.9	0.0822	82.2	-34.2*	0.5203	0.0274	-37.8*
T3R2	2.9	2.6	-	2.8	0.0514	51.4
T3R3	2.9	2.2	-	2.6	0.0496	49.6
T3R4	3.3	3.4	2.6	3.1	0.0864	86.4
T3R5	2.9	3.0	3.2	3.0	0.0884	88.4
T3R6	3.1	2.9	2.2	2.7	0.0744	74.4
T3R7	3.3	3.0	2.8	3.0	0.0879	87.9
T4R1	-	-	-	-	-	-	-75.9*	0.1410	0.0157	-64.4*
T4R2	-	-	-	-	-	-
T4R3	1.6	1.8	1.3	1.6	0.0686	68.6
T4R4	0.5	0.4	0.6	0.5	0.0251	25.1
T4R5	0.9	1.1	1.3	1.1	0.0473	47.3
T4R6	-	-	-	-	-	-
T4R7	-	-	-	-	-	-
T5R1	-	-	-	-	-	-	-85.9*	0.0312	0.0078	-82.3*
T5R2	0.4	0.7	-	0.6	0.0163	16.3
T5R3	-	-	-	-	-	-
T5R4	-	-	-	-	-	-
T5R5	0.8	-	-	0.8	0.0088	8.8
T5R6	-	-	-	-	-	-
T5R7	0.5	-	-	0.5	0.0061	6.1

*: Statistically significant difference in shoot height and dry shoot weight as compared with the vehicle control.

TABLE 1. Tomato DT - Seedling Emergence, Seedling Survival, Shoot Height, and Dry Shoot Weight[c1] [r2]

Group & Replicate	Total no. of seeds emerged	% of Seedling emergence	Seedlings survival on day 14	Total no. of seedlings survival on day 14	% of Seedling survival	8-Aug	9-Aug	10-Aug	11-Aug	12-Aug	13-Aug	Seedling emergence		Plant observations
												*Day 7	*Day 14	*Day 7	*Day 14
												20-Aug	27-Aug	20-Aug	27-Aug
CR1	3	95.2	3	20	100.0	0	0	0	0	0	3	3	3	N(3), 0%(3)	N(3), 0%(3)
CR2	3		3			0	0	0	0	0	3	3	3	N(3), 0%(3)	N(3), 0%(3)
CR3	3		3			0	0	0	0	1	3	3	3	N(3), 0%(3)	N(3), 0%(3)
CR4	3		3			0	0	0	0	1	3	3	3	N(3), 0%(3)	N(3), 0%(3)
CR5	2		2			0	0	0	0	0	2	2	2	N(2), 0%(2)	N(2), 0%(2)
CR6	3		3			0	0	0	0	1	3	3	3	N(3), 0%(3)	N(3), 0%(3)
CR7	3		3			0	0	0	0	0	3	3	3	N(3), 0%(3)	N(3), 0%(3)
VR1	3	95.2	3	20	100.0	0	0	0	0	1	3	3	3	N(3), 0%(3)	N(3), 0%(3)
VR2	2		2			0	0	0	0	1	2	2	2	N(2), 0%(2)	N(2), 0%(2)
VR3	3		3			0	0	0	0	0	3	3	3	N(3), 0%(3)	N(3), 0%(3)
VR4	3		3			0	0	0	0	0	3	3	3	N(3), 0%(3)	N(3), 0%(3)
VR5	3		3			0	0	0	0	1	3	3	3	N(3), 0%(3)	N(3), 0%(3)
VR6	3		3			0	0	0	0	0	3	3	3	N(3), 0%(3)	N(3), 0%(3)
VR7	3		3			0	0	0	0	1	3	3	3	N(3), 0%(3)	N(3), 0%(3)
T1R1	3	95.2	3	20	100.0	0	0	0	0	1	3	3	3	N(3), 0%(3)	N(3), 0%(3)
T1R2	3		3			0	0	0	0	1	3	3	3	N(3), 0%(3)	N(3), 0%(3)
T1R3	2		2			0	0	0	0	0	2	2	2	N(2), 0%(2)	N(2), 0%(2)
T1R4	3		3			0	0	0	0	1	3	3	3	N(3), 0%(3)	N(3), 0%(3)
T1R5	3		3			0	0	0	0	0	3	3	3	N(3), 0%(3)	N(3), 0%(3)
T1R6	3		3			0	0	0	0	1	3	3	3	N(3), 0%(3)	N(3), 0%(3)
T1R7	3		3			0	0	0	0	0	3	3	3	N(3), 0%(3)	N(3), 0%(3)
T2R1	3	81.0	3	17	100.0	0	0	0	0	0	3	3	3	S(3), 10%(2), 30%(1)	S(3), 10%(2), 30%(1)
T2R2	3		3			0	0	0	0	1	3	3	3	N(2), 0%(2), S(1), 25%(1)	N(2), 0%(2), S(1), 25%(1)
T2R3	3		3			0	0	0	0	1	3	3	3	N(3), 0%(3)	N(3), 0%(3)
T2R4	2		2			0	0	0	0	1	2	2	2	N(1), 0%(1), S(1), 35%(1)	N(1), 0%(1), S(1), 35%(1)
T2R5	2		2			0	0	0	0	0	2	2	2	N(2), 0%(2)	N(2), 0%(2)
T2R6	2		2			0	0	0	0	1	2	2	2	N(1), 0%(1), S(1), 45%(1)	N(1), 0%(1), S(1), 45%(1)
T2R7	2		2			0	0	0	0	1	2	2	2	S(2), 30%(2)	S(2), 30%(2)

Note: *: 7 and 14 days after 50% emergence of the seedlings in the control(s) group; N: Normal, S:Stunted growth.

contd. Tomato DT - Seedling Emergence, Seedling Survival, Shoot Height, and Dry Shoot Weight[c1] [r2]

Group & Replicate	Shoot height on day 14				Shoot weight on day 14	Dry shoot weight (mg) on day 14	% Shoot height reduction on day 14	Dry shoot weight (g) on day 14	Average dry shoot weight (g) on day 14	% Dry shoot weight reduction on day 14
	1	2	3	Average	Shoot weight on day 14
	27-Aug				28-Aug
CR1	11.0	10.5	10.0	10.5	0.2212	221.2	-	1.4785	0.0739	-
CR2	10.0	10.5	10.0	10.2	0.2311	231.1
CR3	10.5	8.0	9.5	9.3	0.2202	220.2
CR4	9.5	11.0	10.5	10.3	0.2116	211.6
CR5	9.0	8.2	-	8.6	0.1347	134.7
CR6	10.5	9.5	11.0	10.3	0.2289	228.9
CR7	10.5	11.0	12.5	11.3	0.2308	230.8
VR1	10.2	9.7	8.9	9.6	0.2191	219.1	-	1.4834	0.0742	-
VR2	9.9	10.7	-	10.3	0.1404	140.4
VR3	11.0	9.9	9.6	10.2	0.2316	231.6
VR4	9.9	9.6	10.6	10.0	0.2129	212.9
VR5	10.4	10.6	9.4	10.1	0.2197	219.7
VR6	10.9	9.7	10.7	10.4	0.2306	230.6
VR7	9.8	10.3	9.8	10.0	0.2291	229.1
T1R1	10.3	9.9	10.2	10.1	0.2214	221.4	0.3[YB3]	1.4809	0.0740	-0.2
T1R2	10.1	10.2	9.5	9.9	0.2146	214.6
T1R3	10.6	10.1	-	10.4	0.1469	146.9
T1R4	9.9	11.0	10.3	10.4	0.2389	238.9
T1R5	8.9	10.6	10.5	10.0	0.2169	216.9
T1R6	10.8	9.7	9.9	10.1	0.2214	221.4
T1R7	10.2	9.2	10.4	9.9	0.2208	220.8
T2R1	8.1	8.0	6.8	7.6	0.1823	182.3	-17.3	1.0889	0.0641	-13.6
T2R2	9.2	9.4	7.1	8.6	0.2006	200.6
T2R3	9.5	9.8	10.1	9.8	0.2246	224.6
T2R4	9.2	6.6	-	7.9	0.1179	117.9
T2R5	9.7	9.3	-	9.5	0.1486	148.6
T2R6	9.8	6.3	-	8.1	0.1144	114.4
T2R7	7.1	6.8	-	7.0	0.1005	100.5

contd. Tomato DT - Seedling Emergence, Seedling Survival, Shoot Height, and Dry Shoot Weight[c1] [r2]

Group & Replicate	Total no. of seeds emerged	% of Seedling emergence	Seedlings survival on day 14	Total no. of seedlings survival on day 14	% of Seedling survival	8-Aug	9-Aug	10-Aug	11-Aug	12-Aug	13-Aug	Seedling emergence		Plant observations
												*Day 7	*Day 14	*Day 7	*Day 14
												20-Aug	27-Aug	20-Aug	27-Aug
T3R1	3	66.7	3	14	100.0	0	0	0	0	0	0	3	3	S(3), 60%(3)	S(3), 60%(3)
T3R2	2		2			0	0	0	0	0	0	2	2	S(2), 50%(2)	S(2), 50%(2)
T3R3	1		1			0	0	0	0	0	0	1	1	S(1), 55%(1)	S(1), 55%(1)
T3R4	1		1			0	0	0	0	0	1	1	1	S(1), 50%(1)	S(1), 50%(1)
T3R5	3		3			0	0	0	0	0	1	3	3	S(3), 60%(3)	S(3), 60%(3)
T3R6	2		2			0	0	0	0	0	0	2	2	S(2), 50%(2)	S(2), 50%(2)
T3R7	2		2			0	0	0	0	0	0	2	2	S(2), 60%(2)	S(2), 60%(2)
T4R1	3	57.1	3	12	100.0	0	0	0	0	0	1	3	3	S(3), 80%(3)	S(3), 80%(3)
T4R2	2		2			0	0	0	0	0	0	0	2	-	S(2), 80%(2)
T4R3	1		1			0	0	0	0	0	0	1	1	S(1), 70%(1)	S(1), 70%(1)
T4R4	0		0			0	0	0	0	0	0	0	0	-	-
T4R5	2		2			0	0	0	0	0	0	1	2	S(1), 65%(1)	S(2), 65%(1), 70%(1)
T4R6	3		3			0	0	0	0	0	0	2	3	S(2), 65%(2)	S(3), 65%(2), 75%(1)
T4R7	1		1			0	0	0	0	0	0	1	1	S(1), 50%(1)	S(1), 50%(1)
T5R1	0	23.8	0	5	100.0	0	0	0	0	0	0	0	0	-	-
T5R2	0		0			0	0	0	0	0	0	0	0	-	-
T5R3	1		1			0	0	0	0	0	0	1	1	S(1), 85%(1)	S(1), 85%(1)
T5R4	1		1			0	0	0	0	0	0	0	1	-	S(1), 90%(1)
T5R5	1		1			0	0	0	0	0	0	0	1	-	S(1), 90%(1)
T5R6	0		0			0	0	0	0	0	0	0	0	-	-
T5R7	2		2			0	0	0	0	0	0	0	2	-	S(2), 85%(2)

Note: *: 7 and 14 days after 50% emergence of the seedlings in the control(s) group; S:Stunted growth.

Tomato DT - Seedling Emergence, Seedling Survival, Shoot Height, and Dry Shoot Weight[c1] [r2]

Group & Replicate	Shoot height on day 14				Shoot weight on day 14	Dry shoot weight (mg) on day 14	% Shoot height reduction on day 14	Dry shoot weight (g) on day 14	Average dry shoot weight (g) on day 14	% Dry shoot weight reduction on day 14
	1	2	3	Average	Shoot weight on day 14
	27-Aug				28-Aug
T3R1	5.8	5.6	4.3	5.2	0.1146	114.6	-37.8*	0.6490	0.0464	-37.5*
T3R2	6.6	7.1	-	6.9	0.1006	100.6
T3R3	6.2	-	-	6.2	0.0454	45.4
T3R4	6.8	-	-	6.8	0.0576	57.6
T3R5	5.9	6.3	6.7	6.3	0.1396	139.6
T3R6	7.6	6.6	-	7.1	0.1067	106.7
T3R7	6.0	4.9	-	5.5	0.0845	84.5
T4R1	2.0	2.1	2.2	2.1	0.0661	66.1	-71.8*	0.2802	0.0234	-68.5*
T4R2	1.8	1.9	-	1.9	0.0243	24.3
T4R3	2.8	-	-	2.8	0.0189	18.9
T4R4	-	-	-	-	-	-
T4R5	3.3	2.8	-	3.1	0.0501	50.1
T4R6	3.2	3.4	2.3	3.0	0.0897	89.7
T4R7	4.3	-	-	4.3	0.0311	31.1
T5R1	-	-	-	-	-	-	-86.6*	0.0673	0.0135	-81.9*
T5R2	-	-	-	-	-	-
T5R3	1.6	-	-	1.6	0.0150	15.0
T5R4	1.3	-	-	1.3	0.0131	13.1
T5R5	1.1	-	-	1.1	0.0110	11.0
T5R6	-	-	-	-	-	-
T5R7	1.5	1.3	-	1.4	0.0282	28.2

*: Statistically significant difference in shoot height and dry shoot weight as compared with the vehicle control.

TABLE 1. Soya DT - Seedling Emergence, Seedling Survival, Shoot Height, and Dry Shoot Weight[c1] [r2]

Group & Replicate	Total no. of seeds emerged	% of Seedling emergence	Seedlings survival on day 14	Total no. of seedlings survival on day 14	% of Seedling survival	18-Sep	19-Sep	20-Sep	Seedling emergence		Plant observations
									*Day 7	*Day 14	*Day 7	*Day 14
									27-Sep	04-Oct	27-Sep	04-Oct
CR1	2	95.2	2	20	100.0	0	0	2	2	2	N(2), 0%(2)	N(2), 0%(2)
CR2	3		3			0	0	3	3	3	N(3), 0%(3)	N(3), 0%(3)
CR3	3		3			0	0	3	3	3	N(3), 0%(3)	N(3), 0%(3)
CR4	3		3			0	0	1	3	3	N(3), 0%(3)	N(3), 0%(3)
CR5	3		3			0	0	3	3	3	N(3), 0%(3)	N(3), 0%(3)
CR6	3		3			0	0	3	3	3	N(3), 0%(3)	N(3), 0%(3)
CR7	3		3			0	0	1	3	3	N(3), 0%(3)	N(3), 0%(3)
VR1	2	95.2	2	20	100.0	0	0	2	2	2	N(2), 0%(2)	N(2), 0%(2)
VR2	3		3			0	0	3	3	3	N(3), 0%(3)	N(3), 0%(3)
VR3	3		3			0	0	3	3	3	N(3), 0%(3)	N(3), 0%(3)
VR4	3		3			0	0	1	3	3	N(3), 0%(3)	N(3), 0%(3)
VR5	3		3			0	0	3	3	3	N(3), 0%(3)	N(3), 0%(3)
VR6	3		3			0	0	3	3	3	N(3), 0%(3)	N(3), 0%(3)
VR7	3		3			0	0	1	3	3	N(3), 0%(3)	N(3), 0%(3)
T1R1	3	95.2	3	20	100.0	0	0	0	3	3	N(3), 0%(3)	N(3), 0%(3)
T1R2	3		3			0	0	1	3	3	N(3), 0%(3)	N(3), 0%(3)
T1R3	2		2			0	0	1	2	2	N(2), 0%(2)	N(2), 0%(2)
T1R4	3		3			0	0	2	3	3	N(3), 0%(3)	N(3), 0%(3)
T1R5	3		3			0	0	1	3	3	N(3), 0%(3)	N(3), 0%(3)
T1R6	3		3			0	0	2	3	3	N(3), 0%(3)	N(3), 0%(3)
T1R7	3		3			0	0	1	3	3	N(3), 0%(3)	N(3), 0%(3)
T2R1	3	100.0	3	21	100.0	0	0	2	3	3	S(3), 10%(3)	S(3), 10%(3)
T2R2	3		3			0	0	0	3	3	S(3), 10%(3)	S(3), 10%(3)
T2R3	3		3			0	0	1	3	3	S(3), 10%(3)	S(3), 10%(3)
T2R4	3		3			0	0	0	3	3	S(3), 15%(3)	S(3), 15%(3)
T2R5	3		3			0	0	0	3	3	S(3), 10%(3)	S(3), 10%(3)
T2R6	3		3			0	0	1	3	3	S(3), 10%(3)	S(3), 10%(3)
T2R7	3		3			0	0	0	3	3	S(3), 10%(3)	S(3), 10%(3)

. Soya DT - Seedling Emergence, Seedling Survival, Shoot Height, and Dry Shoot Weight[c1] [r2]

Group & Replicate	Shoot height on day 14				Shoot weight on day 14	Dry shoot weight (mg) on day 14	% Shoot height reduction on day 14	Dry shoot weight (g) on day 14	Average dry shoot weight (g) on day 14	% Dry shoot weight reduction on day 14
	1	2	3	Average	Shoot weight on day 14
	04-Oct				05-Oct
CR1	30.5	30.7	-	30.6	0.4672	467.2	-	4.6632	0.2332	-
CR2	27.1	30.1	31.9	29.7	0.7096	709.6
CR3	30.5	29.2	28.1	29.3	0.6998	699.8
CR4	30.6	27.8	32.5	30.3	0.7089	708.9
CR5	29.8	29.4	30.6	29.9	0.7008	700.8
CR6	31.4	30.0	28.6	30.0	0.7000	700.0
CR7	27.9	30.3	28.6	28.9	0.6769	676.9
VR1	30.5	30.7	-	30.6	0.4673	467.3	-	4.6945	0.2347	-
VR2	27.1	30.1	31.9	29.7	0.7096	709.6
VR3	30.5	29.2	28.1	29.3	0.6999	699.9
VR4	30.6	27.8	32.5	30.3	0.7089	708.9
VR5	29.8	29.4	30.6	29.9	0.7008	700.8
VR6	31.5	30.0	28.6	30.0	0.7210	721.0
VR7	27.9	30.3	28.6	28.9	0.6870	687.0
T1R1	30.1	29.7	28.1	29.3	0.7104	710.4	-1.7	4.7124	0.2356	0.4[YB3]
T1R2	28.7	30.8	30.4	30.0	0.7223	722.3
T1R3	30.1	28.9	-	29.5	0.4776	477.6
T1R4	30.2	28.8	30.1	29.7	0.7021	702.1
T1R5	28.9	29.0	28.6	28.8	0.7016	701.6
T1R6	28.6	30.2	27.9	28.9	0.6913	691.3
T1R7	28.9	29.1	29.0	29.0	0.7071	707.1
T2R1	25.9	26.1	26.4	26.1	0.6400	640.0	-10.8*	4.5977	0.2189	-6.7
T2R2	25.8	25.3	25.9	25.7	0.6220	622.0
T2R3	26.2	27.3	26.9	26.8	0.6757	675.7
T2R4	25.1	25.2	25.9	25.4	0.6367	636.7
T2R5	26.1	28.6	28.3	27.7	0.6806	680.6
T2R6	27.3	28.2	25.6	27.0	0.6646	664.6
T2R7	28.3	26.8	27.6	27.6	0.6781	678.1

*: Statistically significant difference in shoot height and dry shoot weight as compared with the vehicle control.

contd. Soya DT - Seedling Emergence, Seedling Survival, Shoot Height, and Dry Shoot Weight[c1] [r2]

Group & Replicate	Total no. of seeds emerged	% of Seedling emergence	Seedlings survival on day 14	Total no. of seedlings survival on day 14	% of Seedling survival	18-Sep	19-Sep	20-Sep	Seedling emergence		Plant observations
									*Day 7	*Day 14	*Day 7	*Day 14
									27-Sep	04-Oct	27-Sep	04-Oct
T3R1	3	95.2	3	20	100.0	0	0	1	3	3	S(3), 30%(3)	S(3), 30%(3)
T3R2	3		3			0	0	2	3	3	S(3), 25%(3)	S(3), 25%(3)
T3R3	3		3			0	0	2	3	3	S(3), 25%(3)	S(3), 25%(3)
T3R4	3		3			0	0	2	3	3	S(3), 30%(3)	S(3), 30%(3)
T3R5	3		3			0	0	0	3	3	S(3), 25%(3)	S(3), 25%(3)
T3R6	2		2			0	0	2	2	2	S(2), 25%(2)	S(2), 25%(2)
T3R7	3		3			0	0	0	3	3	S(3), 25%(3)	S(3), 25%(3)
T4R1	3	90.5	3	19	100.0	0	0	0	3	3	S(3), 60%(3)	S(3), 60%(3)
T4R2	2		2			0	0	0	2	2	S(2), 60%(2)	S(2), 60%(2)
T4R3	3		3			0	0	1	3	3	S(3), 60%(3)	S(3), 60%(3)
T4R4	3		3			0	0	1	3	3	S(3), 60%(3)	S(3), 60%(3)
T4R5	3		3			0	0	0	3	3	S(3), 60%(3)	S(3), 60%(3)
T4R6	3		3			0	0	1	3	3	S(3), 60%(3)	S(3), 60%(3)
T4R7	2		2			0	0	1	2	2	S(2), 60%(2)	S(2), 60%(2)
T5R1	1	57.1	1	10	83.3	0	0	0	1	1	S(1), 95%(1)	S(1), 95%(1)
T5R2	1		0			0	0	0	1	1	S(1), 95%(1)	D(1), 100%(1)
T5R3	3		3			0	0	0	3	3	S(3), 95%(3)	S(3), 95%(3)
T5R4	3		3			0	0	0	3	3	S(3), 95%(3)	S(3), 95%(3)
T5R5	1		0			0	0	0	1	1	S(1), 95%(1)	D(1), 100%(1)
T5R6	2		2			0	0	1	2	2	S(2), 95%(2)	S(2), 95%(2)
T5R7	1		1			0	0	0	1	1	S(1), 90%(1)	S(1), 90%(1)

Note: *: 7 and 14 days after 50% emergence of the seedlings in the control(s) group; S:Stunted growth; D: Dead.

contd. Soya DT - Seedling Emergence, Seedling Survival, Shoot Height, and Dry Shoot Weight[c1] [r2]

Group & Replicate	Shoot height on day 14				Shoot weight on day 14	Dry shoot weight (mg) on day 14	% Shoot height reduction on day 14	Dry shoot weight (g) on day 14	Average dry shoot weight (g) on day 14	% Dry shoot weight reduction on day 14
	1	2	3	Average	Shoot weight on day 14
	04-Oct				05-Oct
T3R1	20.6	21.0	21.2	20.9	0.5250	525.0	-27.1*	3.5653	0.1783	-24.1
T3R2	20.8	22.8	22.5	22.0	0.5428	542.8
T3R3	21.1	22.0	23.3	22.1	0.5446	544.6
T3R4	19.4	20.3	20.9	20.2	0.4974	497.4
T3R5	21.0	23.1	22.8	22.3	0.5490	549.0
T3R6	22.0	22.7	-	22.4	0.3632	363.2
T3R7	22.8	21.6	22.2	22.2	0.5433	543.3
T4R1	12.3	12.5	12.6	12.5	0.3076	307.6	-56.8*	1.9911	0.1048	-55.4*
T4R2	12.4	13.6	-	13.0	0.2000	200.0
T4R3	12.6	13.1	13.9	13.2	0.3248	324.8
T4R4	11.6	12.1	12.4	12.0	0.2964	296.4
T4R5	12.5	13.7	13.6	13.3	0.3271	327.1
T4R6	13.1	13.5	12.3	13.0	0.3195	319.5
T4R7	13.6	12.9	-	13.3	0.2157	215.7
T5R1	1.3	-	-	1.3	0.0209	20.9	-94.4*	0.2661	0.0266	-88.7*
T5R2	-	-	-	-	-	-
T5R3	1.5	1.2	1.9	1.5	0.0777	77.7
T5R4	1.6	1.9	1.9	1.8	0.0879	87.9
T5R5	-	-	-	-	-	-
T5R6	1.1	2.0	-	1.6	0.0506	50.6
T5R7	2.2	-	-	2.2	0.0290	29.0

*: Statistically significant difference in shoot height and dry shoot weight as compared with the vehicle control.

TABLE 1. Sunflower DT - Seedling Emergence, Seedling Survival, Shoot Height, and Dry Shoot Weight[c1] [r2]

Group & Replicate	Total no. of seeds emerged	% of Seedling emergence	Seedlings survival on day 14	Total no. of seedlings survival on day 14	% of Seedling survival	18-Sep	19-Sep	20-Sep	Seedling emergence		Plant observations
									*Day 7	*Day 14	*Day 7	*Day 14
									27-Sep	04-Oct	27-Sep	04-Oct
CR1	3	100.0	3	21	100.0	0	0	2	3	3	N(3), 0%(3)	N(3), 0%(3)
CR2	3		3			0	0	2	3	3	N(3), 0%(3)	N(3), 0%(3)
CR3	3		3			0	0	3	3	3	N(3), 0%(3)	N(3), 0%(3)
CR4	3		3			0	0	3	3	3	N(3), 0%(3)	N(3), 0%(3)
CR5	3		3			0	0	3	3	3	N(3), 0%(3)	N(3), 0%(3)
CR6	3		3			0	0	1	3	3	N(3), 0%(3)	N(3), 0%(3)
CR7	3		3			0	0	2	3	3	N(3), 0%(3)	N(3), 0%(3)
VR1	3	95.2	3	20	100.0	0	0	1	3	3	N(3), 0%(3)	N(3), 0%(3)
VR2	2		2			0	0	2	2	2	N(2), 0%(2)	N(2), 0%(2)
VR3	3		3			0	0	2	3	3	N(3), 0%(3)	N(3), 0%(3)
VR4	3		3			0	0	2	3	3	N(3), 0%(3)	N(3), 0%(3)
VR5	3		3			0	0	3	3	3	N(3), 0%(3)	N(3), 0%(3)
VR6	3		3			0	0	2	3	3	N(3), 0%(3)	N(3), 0%(3)
VR7	3		3			0	0	3	3	3	N(3), 0%(3)	N(3), 0%(3)
T1R1	3	100.0	3	21	100.0	0	0	1	3	3	N(3), 0%(3)	N(3), 0%(3)
T1R2	3		3			0	0	2	3	3	N(3), 0%(3)	N(3), 0%(3)
T1R3	3		3			0	0	3	3	3	N(3), 0%(3)	N(3), 0%(3)
T1R4	3		3			0	0	2	3	3	N(3), 0%(3)	N(3), 0%(3)
T1R5	3		3			0	0	3	3	3	N(3), 0%(3)	N(3), 0%(3)
T1R6	3		3			0	0	2	3	3	N(3), 0%(3)	N(3), 0%(3)
T1R7	3		3			0	0	2	3	3	N(3), 0%(3)	N(3), 0%(3)
T2R1	2	95.2	2	20	100.0	0	0	2	2	2	S(2), 40%(2)	S(2), 40%(2)
T2R2	3		3			0	0	1	3	3	S(3), 40%(3)	S(3), 40%(3)
T2R3	3		3			0	0	0	3	3	S(3), 40%(3)	S(3), 40%(3)
T2R4	3		3			0	0	1	3	3	S(3), 30%(2), 40%(1)	S(3), 30%(2), 40%(1)
T2R5	3		3			0	0	1	3	3	S(3), 35%(3)	S(3), 35%(3)
T2R6	3		3			0	0	1	3	3	S(3), 35%(3)	S(3), 35%(3)
T2R7	3		3			0	0	0	3	3	S(3), 35%(3)	S(3), 35%(3)

Note: *: 7 and 14 days after 50% emergence of the seedlings in the control(s) group; N: Normal, S:Stunted growth.

contd. Sunflower DT - Seedling Emergence, Seedling Survival, Shoot Height, and Dry Shoot Weight[c1] [r2]

Group & Replicate	Total no. of seeds emerged	% of Seedling emergence	Seedlings survival on day 14	Total no. of seedlings survival on day 14	% of Seedling survival	18-Sep	19-Sep	20-Sep	Seedling emergence		Plant observations
									*Day 7	*Day 14	*Day 7	*Day 14
									27-Sep	04-Oct	27-Sep	04-Oct
T3R1	3	95.2	3	20	100.0	0	0	0	3	3	S(3), 45%(3)	S(3), 45%(3)
T3R2	3		3			0	0	1	3	3	S(3), 40%(3)	S(3), 40%(3)
T3R3	3		3			0	0	0	3	3	S(3), 50%(3)	S(3), 50%(3)
T3R4	2		2			0	0	0	2	2	S(2), 50%(2)	S(2), 50%(2)
T3R5	3		3			0	0	0	3	3	S(3), 40%(3)	S(3), 40%(3)
T3R6	3		3			0	0	0	3	3	S(3), 50%(3)	S(3), 50%(3)
T3R7	3		3			0	0	0	3	3	S(3), 40%(3)	S(3), 40%(3)
T4R1	2	95.2	2	20	100.0	0	0	0	2	2	S(2), 70%(2)	S(2), 70%(2)
T4R2	3		3			0	0	0	3	3	S(3), 70%(2), 80%(1)	S(3), 70%(2), 80%(1)
T4R3	3		3			0	0	0	3	3	S(3), 70%(3)	S(3), 70%(3)
T4R4	3		3			0	0	0	3	3	S(3), 70%(2), 80%(1)	S(3), 70%(2), 80%(1)
T4R5	3		3			0	0	0	3	3	S(3), 70%(2), 80%(1)	S(3), 70%(2), 80%(1)
T4R6	3		3			0	0	1	3	3	S(3), 70%(3)	S(3), 70%(3)
T4R7	3		3			0	0	0	3	3	S(3), 70%(2), 80%(1)	S(3), 70%(2), 80%(1)
T5R1	2	23.8	0	2	40.0	0	0	0	2	2	S(2), 95%(2)	D(2), 100%(2)
T5R2	0		0			0	0	0	0	0	-	-
T5R3	2		2			0	0	0	2	2	S(2), 95%(2)	S(2), 95%(2)
T5R4	1		0			0	0	0	1	1	S(1), 95%(1)	D(1), 100%(1)
T5R5	0		0			0	0	0	0	0	-	-
T5R6	0		0			0	0	0	0	0	-	-
T5R7	0		0			0	0	0	0	0	-	-

Note: *: 7 and 14 days after 50% emergence of the seedlings in the control(s) group; S:Stunted growth; D: Dead.

contd. Sunflower DT - Seedling Emergence, Seedling Survival, Shoot Height, and Dry Shoot Weight[c1] [r2]

Group & Replicate	Shoot height on day 14				Shoot weight on day 14	Dry shoot weight (mg) on day 14	% Shoot height reduction on day 14	Dry shoot weight (g) on day 14	Average dry shoot weight (g) on day 14	% Dry shoot weight reduction on day 14
	1	2	3	Average	Shoot weight on day 14
	04-Oct				05-Oct
CR1	19.6	21.9	22.2	21.2	0.6017	601.7	-	4.3684	0.2080	-
CR2	20.8	21.4	19.4	20.5	0.6269	626.9
CR3	20.1	20.2	20.2	20.2	0.6105	610.5
CR4	20.9	22.8	21.8	21.8	0.6355	635.5
CR5	19.7	20.2	20.5	20.1	0.6334	633.4
CR6	20.5	20.6	22.5	21.2	0.6261	626.1
CR7	21.8	24.2	19.4	21.8	0.6343	634.3
VR1	18.8	18.6	18.3	18.6	0.5876	587.6	-	4.1870	0.2094	-
VR2	19.4	19.9	-	19.7	0.4564	456.4
VR3	21.2	22.2	17.9	20.4	0.6211	621.1
VR4	20.6	21.4	18.1	20.0	0.6198	619.8
VR5	22.0	19.5	21.5	21.0	0.6378	637.8
VR6	20.5	19.9	20.6	20.3	0.6276	627.6
VR7	19.6	21.6	22.0	21.1	0.6367	636.7
T1R1	19.2	19.6	22.6	20.5	0.5979	597.9	2.9[YB3]	4.3189	0.2057	-1.8
T1R2	19.6	17.4	18.7	18.6	0.5869	586.9
T1R3	20.3	21.1	19.9	20.4	0.6006	600.6
T1R4	17.6	20.8	22.0	20.1	0.5987	598.7
T1R5	19.6	21.9	21.8	21.1	0.6345	634.5
T1R6	22.7	22.1	20.9	21.9	0.6459	645.9
T1R7	22.9	22.6	22.4	22.6	0.6544	654.4
T2R1	12.6	13.1	-	12.9	0.2809	280.9	-33.4*	2.9307	0.1465	-30.0*
T2R2	12.4	14.8	12.3	13.2	0.4198	419.8
T2R3	12.6	13.9	12.6	13.0	0.4227	422.7
T2R4	14.2	14.7	12.9	13.9	0.4724	472.4
T2R5	13.5	13.6	14.5	13.9	0.4632	463.2
T2R6	13.6	13.9	13.5	13.7	0.4398	439.8
T2R7	13.1	13.5	13.9	13.5	0.4319	431.9

*: Statistically significant difference in shoot height and dry shoot weight as compared with the vehicle control.

contd. Sunflower DT - Seedling Emergence, Seedling Survival, Shoot Height, and Dry Shoot Weight[c1] [r2]

Group & Replicate	Shoot height on day 14				Shoot weight on day 14	Dry shoot weight (mg) on day 14	% Shoot height reduction on day 14	Dry shoot weight (g) on day 14	Average dry shoot weight (g) on day 14	% Dry shoot weight reduction on day 14
	1	2	3	Average	Shoot weight on day 14
	04-Oct				05-Oct
T3R1	12.6	12.4	12.1	12.4	0.4081	408.1	-41.6*	2.5427	0.1271	-39.3*
T3R2	13.6	12.9	12.6	13.0	0.3987	398.7
T3R3	10.8	10.1	11.3	10.7	0.3763	376.3
T3R4	11.6	10.1	-	10.9	0.2574	257.4
T3R5	12.9	12.1	12.6	12.5	0.3898	389.8
T3R6	10.3	10.9	9.5	10.2	0.3339	333.9
T3R7	12.3	12.9	12.7	12.6	0.3785	378.5
T4R1	6.3	6.8	-	6.6	0.1513	151.3	-70.6*	1.3591	0.0680	-67.5*
T4R2	6.2	4.6	6.8	5.9	0.2119	211.9
T4R3	6.2	6.1	5.1	5.8	0.2017	201.7
T4R4	6.4	6.6	4.1	5.7	0.1983	198.3
T4R5	6.5	4.2	6.4	5.7	0.1901	190.1
T4R6	6.1	6.2	6.8	6.4	0.2231	223.1
T4R7	6.8	5.0	4.5	5.4	0.1827	182.7
T5R1	-	-	-	-	-	-	-93.8*	0.0501	0.0251	-88.0*
T5R2	-	-	-	-	-	-
T5R3	1.1	1.4	-	1.3	0.0501	50.1
T5R4	-	-	-	-	-	-
T5R5	-	-	-	-	-	-
T5R6	-	-	-	-	-	-
T5R7	-	-	-	-	-	-

*: Statistically significant difference in shoot height and dry shoot weight as compared with the vehicle control.

Validity criteria fulfilled:

yes

Remarks:

• The seedling emergence was at least 90% in all the tested plant species; mean survival of emerged control(s) seedlings 100% • The plants exhibited only normal variation in growth and morphology; • Environmental conditions and growth media are same

Conclusions:

Based on the outcomes of definitive test by concerning the emergence, the shoot length, and the dry weight were statistically analyzed to determine the EC10, EC20, EC50, EC90, NOEC and LOEC. The NOEC values based on the emergence, the shoot length, and the dry weight, are 45, 45, 45 (onion), 200, 80, 200 (corn), 200,32,80 (cabbage), 5,15,15 (tomato), 135,15, 135 (soybean) and 405, 15, 15sunflower

Executive summary:

The study aimed at evaluating the effect of test item on seedling emergence and seedling growth of 6 terrestrial plants was conducted on 2 monocotyledonous and 4 dicotyledonous species. The test item was mixed into the acid washed quartz sand (artificial substrate/soil which was used as the test medium) and exposed as per test guidelines.

A range finding test was carried out with the plant species (onion, corn, cabbage, tomato, soybean, and sunflower) at 10, 100, 500 and 1000 mg test item/kg dry weight of artificial substrate along with vehicle control to determine test concentrations for the definitive test. Effects of test item were observed on seedling emergence, shoot height, and dry shoot weight on day 14 in all the tested plant species.

Based on the range finding results for each plant species, five test concentrations of test item were tested in main study. There was also adequate number of concurrent control and vehicle control (acetone) group. Seeds of the test species were sown in plastic pots with 2 seeds/pot (corn), 3 seeds/pot (cabbage, tomato, soybean, and sunflower) and 5 seeds/pot (onion). Uniform seeds of the test plant species were sown in chemically inert plastic pots containing the test artificial substrate. Within a given phase of the test, all test plants including the control(s) were from the same source. The plants were grown in non-porous plastic pots with a tray saucer under the pot. The pots were large enough to allow normal growth. A pot was defined as the replicate (diameter: 15.5 cm, surface area about: 189 cm2)

To prepare required test item concentration, required quantity of test item was weighed and mixed with acetone (sonication was made, where essential) followed by introduction into artificial substrate. Vehicle was allowed to dry completely, and this was homogenously mixed using blender. For control, required quantity of artificial substrate was mixed thoroughly with nutrient solution. For vehicle control, required quantity of artificial substrate was mixed with acetone and allowed to dry completely and this was homogenously mixed using blender. After test medium preparation, the pots were filled with uniform quantity of test medium (2 kg). This was followed by addition of required volume (220 mL/pot) of water based on WHCmax (26%) of the artificial substrate.

The concentrations were separated by for of 3 for onion soybean sunflower and tomato, and 2.5 for corn and cabbage. The test concentrations for onion, soybean and sunflower are 15, 45, 135, 405, 1215 mg/kg/dw. For corn and cabbage are 32, 80, 200, 500,1250 mg/kg/dw. For tomato are 5, 15, 45, 135, 405 mg/kg/dw. The experiment was conducted in a special room with PAR lights with light intensity22600 to 24100 Lux, and suitable environmental conditions withair temperature of 19.5 to 23.3 °C and air humidity of 71 to 89 %were provided for plant species. During the experiment, the plants were observed for emergence (every day and then at 7 days interval) and visual phytotoxicity (at 7 and 14 days). The experiment completed on 14 days after the emergence of 50% of the vehicle control seedlings. At the end of the experiment, the number of surviving plants, their height and dry weight were observed.

Analytical verification of the test medium (test item active ingredient analysis in test medium) was performed using a validated analytical method to determine the test item. The samples from the low, mid and high-test item concentrations were analyzed at the beginning of the test (day 0) along with the control(s). For analysis, required quantity of test medium was sampled on day 0

During the observation period, i.e. 14 days after 50% of the control(s) plants have emerged, the plants were observed frequently for emergence and visual phytotoxicity and mortality. At the end of the test, measurement of percent emergence and biomass of surviving plants were recorded, as well as visible detrimental effects on different parts of the plant. The latter include abnormalities in appearance of the emerged seedlings, stunted growth, chlorosis, discoloration, mortality, and effects on plant development. A uniform scoring system for visual injury was used to evaluate the observable toxic responses. The final biomass was measured using final average dry shoot weight of surviving plants, by harvesting the shoot at the soil surface and drying them to constant weight at 60 to 70 ºC. The height of the shoot of surviving plants were measured at the end of the test.

Plant observations on days 7 and 14 recorded normal in control and vehicle control. In certain test item treated groups exhibited visual phytotoxicity (toxic responses) of stunted growth and mortality (except onion, cabbage, and tomato). Effects of test item were observed on seedling emergence, seedling survival (except onion, cabbage, and tomato), shoot height, and dry shoot weight on day 14 in all the tested plant species.

Visual assessment is the rating of visual damage based on observations of plant stand, vigour, malformation, chlorosis, necrosis, and overall appearance compared with control(s). Uniform scoring system was used for visual injury to evaluate the observable toxic responses. Plants were observed for severity of the visual phytotoxic effects of test item from the grading scale of 0 to 100 %.

A dose-response relationship was established in terms of a regression equation by using four parameter Model. Different model was used for estimating ECx (EC10, EC20, EC50, EC90) and its confidence limits, where possible. The statistical analysis of the shoot height and shoot weight data was evaluated using licensed copies of SYSTAT Statistical package version 12.0. Data was tested for normality (Shapiro-Wilk test) and homogeneity of variance (Levene’s) before performing further analysis. When data was found as normal and homogeneous, ANOVA was performed, when data was found as non-normal or non-homogeneous, data were transformed, and ANOVA was done on transformed data for overall group comparison. Dunnett’s test was performed for pairwise comparison. Comparison of two group’s mean was done using two-sample t-test.

The present study can be considered valid since, the control(s) satisfies the validity criteria given in the guideline as below.

· The seedling emergence was at least 90% in all the tested plant species (acceptable criterion: seedling emergence should be at least 70%).

· The seedlings didn’t exhibited visible phytotoxic effects and the plants exhibited only normal variation in growth and morphology for particular species (acceptable criterion: seedlings do not exhibit visible phytotoxic effects and the plants exhibit only normal variation in growth and morphology for particular species).

· The mean survival of emerged control(s) seedlings was at least 100% for the duration of the study (acceptable criterion: mean survival of emerged control(s) seedlings should be at least 90% for the duration of the study).

· Environmental conditions for a particular species was identical and growing media contained the same amount of substrate in all the tested plant species from the same source (acceptable criterion: environmental conditions for a particular species should be identical and growing media should contain the same amount of substrate from the same source)

Description of key information

A dose-response relationship was established in terms of a regression equation by using four parameter Model. Different model was used for estimating ECx (EC10, EC20, EC50, EC90) and its confidence limits, where possible. The statistical analysis of the shoot height and shoot weight data was evaluated using licensed copies of SYSTAT Statistical package version 12.0. Data was tested for normality (Shapiro-Wilk test) and homogeneity of variance (Levene’s) before performing further analysis. When data was found as normal and homogeneous, ANOVA was performed, when data was found as non-normal or non-homogeneous, data were transformed, and ANOVA was done on transformed data for overall group comparison. Dunnett’s test was performed for pairwise comparison. Comparison of two group’s mean was done using two-sample t-test.

The present study can be considered valid since, the control(s) satisfies the validity criteria given in the guideline as below.

· The seedling emergence was at least 90% in all the tested plant species (acceptable criterion: seedling emergence should be at least 70%).

Key value for chemical safety assessment

Long-term EC10, LC10 or NOEC for terrestrial plants:: 5 mg/kg soil dw

Additional information

A dose-response relationship was established in terms of a regression equation by using four parameter Model. Different model was used for estimating ECx (EC10, EC20, EC50, EC90) and its confidence limits, where possible. The statistical analysis of the shoot height and shoot weight data was evaluated using licensed copies of SYSTAT Statistical package version 12.0. Data was tested for normality (Shapiro-Wilk test) and homogeneity of variance (Levene’s) before performing further analysis. When data was found as normal and homogeneous, ANOVA was performed, when data was found as non-normal or non-homogeneous, data were transformed, and ANOVA was done on transformed data for overall group comparison. Dunnett’s test was performed for pairwise comparison. Comparison of two group’s mean was done using two-sample t-test.

The present study can be considered valid since, the control(s) satisfies the validity criteria given in the guideline as below.

· The seedling emergence was at least 90% in all the tested plant species (acceptable criterion: seedling emergence should be at least 70%).

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