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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Skin irritation / corrosion

Currently viewing:

Administrative data

Endpoint:
skin irritation: in vivo
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1986-10-21 to 1986-11-04
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1986
Report date:
1986

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
OECD Guideline 404 (Acute Dermal Irritation / Corrosion)
Qualifier:
according to guideline
Guideline:
EU Method B.4 (Acute Toxicity: Dermal Irritation / Corrosion)
GLP compliance:
yes

Test material

Constituent 1
Reference substance name:
2,4-Dichlorobenzotrichloride
IUPAC Name:
2,4-Dichlorobenzotrichloride
Constituent 2
Chemical structure
Reference substance name:
2,4-dichloro-1-(trichloromethyl)benzene
EC Number:
235-868-1
EC Name:
2,4-dichloro-1-(trichloromethyl)benzene
Cas Number:
13014-18-1
Molecular formula:
C7H3Cl5
IUPAC Name:
2,4-dichloro-1-(trichloromethyl)benzene
Constituent 3
Reference substance name:
2,4-Dichlor-1-trichlormethylbenzol
IUPAC Name:
2,4-Dichlor-1-trichlormethylbenzol
Test material form:
solid: crystalline
Details on test material:
Please refer to confidential details on test material

Test animals

Species:
rabbit
Strain:
New Zealand White
Details on test animals or test system and environmental conditions:
TEST ANIMALS
- Source: HOECHST AG, Kastengrund
- Age at study initiation: ca. 3 - 5 months
- Weight at study initiation: 1.8 - 2.9 kg
- Housing: individually
- Diet: Altromin 2123 ad libitum
- Water: ad libitum

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 20 +/- 3
- Humidity (%): 50 +/- 20
- Photoperiod (hrs dark / hrs light): 12/12

Test system

Type of coverage:
semiocclusive
Preparation of test site:
shaved
Vehicle:
other: Polyethylene glycol (PEG) 400
Amount / concentration applied:
500mg mixed wiht 0.3 ml PEG 400
Duration of treatment / exposure:
4 hours
Observation period:
30-60 min, 24 hours, 48 hours, 72 hours as well as 7 and 14 days after patch removal.
Number of animals:
3
Details on study design:
TEST SITE
- Area of exposure: 6.25 cm²
- Type of wrap if used: semiocclusive bandage


REMOVAL OF TEST SUBSTANCE
- Washing: yes, with water
- Time after start of exposure: after 4 hours

Results and discussion

In vivo

Resultsopen allclose all
Irritation parameter:
erythema score
Basis:
animal #1
Time point:
24/48/72 h
Score:
1.7
Max. score:
4
Reversibility:
fully reversible within: 14 days
Irritation parameter:
erythema score
Basis:
animal #2
Time point:
24/48/72 h
Score:
2
Max. score:
4
Reversibility:
fully reversible within: 14 days
Irritation parameter:
erythema score
Basis:
animal #3
Time point:
24/48/72 h
Score:
3.3
Max. score:
4
Reversibility:
fully reversible within: 14 days
Irritation parameter:
edema score
Basis:
animal #1
Time point:
24/48/72 h
Score:
0
Max. score:
4
Reversibility:
fully reversible within: 14 days
Irritation parameter:
edema score
Basis:
animal #2
Time point:
24/48/72 h
Score:
1.7
Max. score:
4
Reversibility:
fully reversible within: 14 days
Irritation parameter:
edema score
Basis:
animal #3
Time point:
24/48/72 h
Score:
0
Max. score:
4
Reversibility:
fully reversible within: 14 days

Applicant's summary and conclusion

Interpretation of results:
irritating
Remarks:
Migrated information Criteria used for interpretation of results: other: Directive 67/548/EEC (DSD)
Conclusions:
Under the conditions of the present study 2,4-Dichlorobenzotrichloride induced skin irritation in rabbits.