Octa-1,7-diene

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Basic data given

Data source

Reference

Reference 1

Reference Type:

publication

Title:

Range-Finding Toxicity Data: List VII

Author:

Smyth H.F.; Carpenter, C.P.; Weil C.S.; Pozzani, U.C.; Striegel, J.A.; Nycum, J.S.

Year:

1969

Bibliographic source:

American Industrial Hygiene Association Journal 30: 470 - 476

Reference 2

Reference Type:

publication

Title:

Range-Finding Toxicity Data: List VI

Author:

Smyth H.F.; Carpenter, C.P.; Weil C.S.; Pozzani, U.C.; Striegel, J.A.

Year:

1962

Bibliographic source:

American Industrial Hygiene Association Journal 23: 95 - 107

Materials and methods

Principles of method if other than guideline:

6 rats were exposed via inhalation (whole body) to the vapour of the test substance at a concentration of 8000 ppm for 4 h followed by a 14-day observation period. Addionally, rats were exposed to an atmosphere saturated with vapour of the test substance for different time periods (15 min up to 8 h) in order to determine the longest inhalation period which permitted all rats to survive the 2-week observation period.

GLP compliance:

no

Remarks:

study performed prior to implementation of GLP

Test type:

standard acute method

Test material

Test material information

Constituent 1

Reference substance name:

Octa-1,7-diene

EC Number:

223-054-9

EC Name:

Octa-1,7-diene

Cas Number:

3710-30-3

Molecular formula:

C8H14

IUPAC Name:

octa-1,7-diene

Test animals

Species:

rat

Strain:

not specified

Sex:

not specified

Administration / exposure

Route of administration:

inhalation: vapour

Type of inhalation exposure:

whole body

Vehicle:

air

Details on inhalation exposure:

GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION (concentrated vapour inhalation)
- Exposure apparatus: sealed chamber
- Exposure chamber volume: 120 L
- Source and rate of air: passing 2.5 L/min of dried air through a fritted glass disc immersed to a depth of at least 2.54 cm in approx. 50 mL of the test substance contained in a gas-washing bottle
- Temperature in air chamber: room temperature

GENERATION OF TEST ATMOSPHERE (inhalation of metered vapour concentration)
- Source and rate of air: flowing stream of vapour prepared by various styles of proportioning pumps

TEST ATMOSPHERE
- Brief description of analytical method used: no analytics performed
- Samples taken from breathing zone: no

Analytical verification of test atmosphere concentrations:

no

Duration of exposure:

4 h

Remarks on duration:

Exposure to 8000 ppm; Additionally, treatments with concentrated vapour were performed for several other time periods (15 min - 8 h).

Concentrations:

Metered vapour concentration: 8000 ppm (corresponding to 36 mg/L)
Saturated vapour concentration at 20 °C: 129.803 mg/L (QSAR; Danish EPA Database, 2004)

No. of animals per sex per dose:

6 animals

Control animals:

not specified

Details on study design:

- Duration of observation period following administration: 14 days

Results and discussion

Effect levels

Sex:

not specified

Dose descriptor:

LC50

Effect level:

> 36 mg/L air (nominal)

Based on:

test mat.

Exp. duration:

4 h

Remarks on result:

other: 8000 ppm; 2/6 animals died

Mortality:

2/6 animals died by inhalation of the metered vapour concentration of 8000 ppm with an exposure duration of 4 h within the 14-day observation period.
No deaths occurred when the rats were exposed for 15 min to concentrated vapour of the test substance.

Applicant's summary and conclusion

Interpretation of results:

other: inconclusive

Remarks:

Criteria used for interpretation of results: OECD GHS

Conclusions:

In an acute inhalation study, 2/6 rats died after exposure to a vapour concentration of 8000 ppm (corresponding to 36 mg/L air) for 4 h. Thus the LC50 value was assumed to be greater than 36 mg/L.