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Administrative data

Endpoint:
acute toxicity: inhalation
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Basic data given

Data source

Referenceopen allclose all

Reference Type:
publication
Title:
Range-Finding Toxicity Data: List VII
Author:
Smyth H.F.; Carpenter, C.P.; Weil C.S.; Pozzani, U.C.; Striegel, J.A.; Nycum, J.S.
Year:
1969
Bibliographic source:
American Industrial Hygiene Association Journal 30: 470 - 476
Reference Type:
publication
Title:
Range-Finding Toxicity Data: List VI
Author:
Smyth H.F.; Carpenter, C.P.; Weil C.S.; Pozzani, U.C.; Striegel, J.A.
Year:
1962
Bibliographic source:
American Industrial Hygiene Association Journal 23: 95 - 107

Materials and methods

Principles of method if other than guideline:
6 rats were exposed via inhalation (whole body) to the vapour of the test substance at a concentration of 8000 ppm for 4 h followed by a 14-day observation period. Addionally, rats were exposed to an atmosphere saturated with vapour of the test substance for different time periods (15 min up to 8 h) in order to determine the longest inhalation period which permitted all rats to survive the 2-week observation period.
GLP compliance:
no
Remarks:
study performed prior to implementation of GLP
Test type:
standard acute method

Test material

Constituent 1
Chemical structure
Reference substance name:
Octa-1,7-diene
EC Number:
223-054-9
EC Name:
Octa-1,7-diene
Cas Number:
3710-30-3
Molecular formula:
C8H14
IUPAC Name:
octa-1,7-diene

Test animals

Species:
rat
Strain:
not specified
Sex:
not specified

Administration / exposure

Route of administration:
inhalation: vapour
Type of inhalation exposure:
whole body
Vehicle:
air
Details on inhalation exposure:
GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION (concentrated vapour inhalation)
- Exposure apparatus: sealed chamber
- Exposure chamber volume: 120 L
- Source and rate of air: passing 2.5 L/min of dried air through a fritted glass disc immersed to a depth of at least 2.54 cm in approx. 50 mL of the test substance contained in a gas-washing bottle
- Temperature in air chamber: room temperature

GENERATION OF TEST ATMOSPHERE (inhalation of metered vapour concentration)
- Source and rate of air: flowing stream of vapour prepared by various styles of proportioning pumps

TEST ATMOSPHERE
- Brief description of analytical method used: no analytics performed
- Samples taken from breathing zone: no
Analytical verification of test atmosphere concentrations:
no
Duration of exposure:
4 h
Remarks on duration:
Exposure to 8000 ppm; Additionally, treatments with concentrated vapour were performed for several other time periods (15 min - 8 h).
Concentrations:
Metered vapour concentration: 8000 ppm (corresponding to 36 mg/L)
Saturated vapour concentration at 20 °C: 129.803 mg/L (QSAR; Danish EPA Database, 2004)
No. of animals per sex per dose:
6 animals
Control animals:
not specified
Details on study design:
- Duration of observation period following administration: 14 days

Results and discussion

Effect levels
Sex:
not specified
Dose descriptor:
LC50
Effect level:
> 36 mg/L air (nominal)
Based on:
test mat.
Exp. duration:
4 h
Remarks on result:
other: 8000 ppm; 2/6 animals died
Mortality:
2/6 animals died by inhalation of the metered vapour concentration of 8000 ppm with an exposure duration of 4 h within the 14-day observation period.
No deaths occurred when the rats were exposed for 15 min to concentrated vapour of the test substance.

Applicant's summary and conclusion

Interpretation of results:
other: inconclusive
Remarks:
Criteria used for interpretation of results: OECD GHS
Conclusions:
In an acute inhalation study, 2/6 rats died after exposure to a vapour concentration of 8000 ppm (corresponding to 36 mg/L air) for 4 h. Thus the LC50 value was assumed to be greater than 36 mg/L.