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Short-term toxicity to fish

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Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
07/06/1981 - 07/10/1981
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Qualifier:
no guideline followed
Principles of method if other than guideline:
Materials and methods are well described. Thus the study can be considered as reliable.
GLP compliance:
not specified
Specific details on test material used for the study:
concentration of stock solution: 15.8 g/L
Vehicle:
no
Test organisms (species):
Pimephales promelas
Details on test organisms:
age: 33 days
mean length: 22.0 mm
SD length: 2.708 mm
mean weight: 0.152 g
SD weight: 0.0703 g
loading: 0.603 g/L
Water media type:
freshwater
Total exposure duration:
96 h
Hardness:
45.0 mg CaCO3/L
Test temperature:
25.4 °C
pH:
7.42
Dissolved oxygen:
7.5 mg/L
Details on test conditions:
Tank volume: 6.3 L
Alkalinity: 44.0 mg CaCO3/L
25 animals per tank
2 tests for each concentration
Reference substance (positive control):
not specified
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
15.4 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Key result
Duration:
96 h
Dose descriptor:
EC50
Effect conc.:
15.2 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
behaviour
Remarks:
general effects
Sublethal observations / clinical signs:

mortalities reported (25 animals per tank):

 hours  control  A  B  C  D  E
 3  0 / 0  0 / 0  0 / 0  0 / 0  0 / 0  0 / 0
 5  0 / 0  0 / 0  0 / 0  0 / 0  0 / 0  4 / 5
 20  0 / 0  0 / 0  0 / 0  4 / 5  19 / 21  24 / 25
 24  0 / 0  0 / 0  0 / 0  4 / 6  20 / 23  25 / 25
 44  0 / 0  0 / 0  1 / 1  11 / 16  25 / 25  25 / 25
 48  0 / 0  0 / 0  1 / 1  12 / 16  25 / 25  25 / 25
 72  0 / 0  0 / 0  1 / 1  16 / 17  25 / 25 25 / 25
 96 0 / 0  0 / 0  1 / 1  19 / 17  25 / 25  25 / 25

LC50, 96 h: 15.4 mg/L

conf. lim.: 14.1 - 16.9 mg/L

effect reported (25 animals per tank):

hours  control  A  B  C  D  E
 3  0 / 0  0 / 0  0 / 0  0 / 0  0 / 0  2 / 2
 5  0 / 0  0 / 0  0 / 0  0 / 0  0 / 1  14 / 14
 20  0 / 0  0 / 0  0 / 0  4 / 9  20 / 21  24 / 25
 24  0 / 0  0 / 0  0 / 0  7 / 9  22 / 24  25 / 25
 44  0 / 0  0 / 0  1 / 1  15 / 17  25 / 25  25 / 25
 48  0 / 0  0 / 0  1 / 1  15 / 18  25 / 25  25 / 25
 72  0 / 0  0 / 0  1 / 1  16 / 18  25 / 25 25 / 25
 96 0 / 0  0 / 0  1 / 1  19 / 18  25 / 25  25 / 25

EC50, 96 h: 15.2 mg/L

conf. lim.: 14.0 - 16.6 mg/L

Validity criteria fulfilled:
yes
Conclusions:
Based on the test results a LC50, 96 h of 15.4 mg/L with confidential limits of 14.1 - 16.9 mg/L can be stated.
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
05/09/1983 - 05/13/1983
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Qualifier:
no guideline followed
Principles of method if other than guideline:
Materials and methods are well described. Thus the study can be considered as reliable.
GLP compliance:
not specified
Specific details on test material used for the study:
concentration of stock solution: 260 mg/L
Vehicle:
no
Test organisms (species):
Pimephales promelas
Details on test organisms:
age: 28 days
mean length: 19.8 mm
SD length: 2.149 mm
mean weight: 0.120 g
SD weight: 0.0324 g
loading: 1.200 g/L
Water media type:
freshwater
Total exposure duration:
96 h
Hardness:
37.4 mg CaCO3/L
Test temperature:
25.1 °C
pH:
7.68
Dissolved oxygen:
7.1 mg/L
Details on test conditions:
Tank volume: 2.0 L
Alkalinity: 38.6 mg CaCO3/L
20 animals per tank
1 test for each concentration
Reference substance (positive control):
not specified
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
17.4 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Key result
Duration:
96 h
Dose descriptor:
EC50
Effect conc.:
16.8 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
not specified
Remarks:
general effects
Sublethal observations / clinical signs:

mortalities reported (20 animals per tank):

 hours  control  A  B  C  D  E
 20  0  0  0  1  12  20
 24  0  0  0  2  14  20
 44  0  0  0  3  16  20
 48  0  0  0  7  16  20
 72  0  0  1  12  17 20
 92 0  0  4  14  19  20
 96  0  0  5  14  20  20

LC50, 96 h: 17.4 mg/L

conf. lim.: 15.4 - 19.5 mg/L

effect reported (20 animals per tank):

hours  control  A  B  C  D  E
 20  0  0  0  3  16  20
 24  0  0  0  6  16  20
 44  0  0  0  11  17  20
 48  0  0  1  12  16  20
 72  0  0  1  14  19 20
 92 0  0  5  14  20  20
 96  0  0  7  14  20  20

EC50, 96 h: 16.8 mg/L

conf. lim.: 14.9 - 18.9 mg/L

Validity criteria fulfilled:
yes
Conclusions:
Based on the test results a LC50, 96 h of 17.4 mg/L with confidential limits of 15.4 - 19.5 mg/L can be stated.
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
05/21/1985 - 05/25/1985
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Qualifier:
no guideline followed
Principles of method if other than guideline:
Materials and methods are well described. Thus the study can be considered as reliable.
GLP compliance:
not specified
Specific details on test material used for the study:
concentration of stock solution: 500 mg/L
Vehicle:
no
Test organisms (species):
Pimephales promelas
Details on test organisms:
age: 28 days
mean length: 22.0 mm
SD length: 1.432 mm
mean weight: 0.165 g
SD weight: 0.0338 g
loading: 2.750 g/L
Water media type:
freshwater
Total exposure duration:
96 h
Hardness:
53.0 mg CaCO3/L
Test temperature:
23.9 °C
pH:
7.27
Dissolved oxygen:
7.0 mg/L
Details on test conditions:
Tank volume: 1.2 L
Alkalinity: 41.1 mg CaCO3/L
20 animals per tank
1 test for each concentration
Reference substance (positive control):
not specified
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
11.8 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Key result
Duration:
96 h
Dose descriptor:
EC50
Effect conc.:
11.1 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
not specified
Remarks:
general effects
Sublethal observations / clinical signs:

mortalities reported (20 animals per tank):

 hours  control  A  B  C  D  E
 24  0  0  0  0  9  18
 48  0  0  0  12  19  20
 72  0  0  4  17  19 20
 96 0  2  5  17  20  20

LC50, 96 h: 11.8 mg/L

conf. lim.: 10.3 - 13.4 mg/L

effect reported (20 animals per tank):

hours  control  A  B  C  D  E
 24  0  0  0  3  15  20
 48  0  0  1  17  19  20
 72  0  1  4  17  19 20
 96 0  2  7  17  20  20

EC50, 96 h: 11.1 mg/L

conf. lim.: 9.65 - 12.8 mg/L

Validity criteria fulfilled:
yes
Conclusions:
Based on the test results a LC50, 96 h of 11.8 mg/L with confidential limits of 10.3 - 13.4 mg/L can be stated.

Description of key information

Three independent short-term toxicity tests were conducted with diethyl malonate on pimephales promelas.

The LC50, 96 h values determined by the studies were in the range of 11.8 - 17.4 mg/L.

Thus the LC50 for fish for diethylmalonate can be stated as >= 11.8 mg/L.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
11.8 mg/L

Additional information