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EC number: 607-104-0 | CAS number: 225375-65-5
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1991-08-12 to 1992-01-24
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: OECD Guideline test under GLP
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 991
- Report date:
- 1992
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Version / remarks:
- 92/69/EWG, C.1
- GLP compliance:
- yes
Test material
- Reference substance name:
- Silicophosphonat
- IUPAC Name:
- Silicophosphonat
- Details on test material:
- - Name of test material (as cited in study report): Na-silicophosphonat
- Physical state: Liquid
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Nominal concentrations (based on rangefinding studies): 50, 100, 464, 1000, 2150, 4640 and 10000 mg/L
- 10000 mg/L was tested in parallel after pH-adjustement
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
-The product was added to the test water without any pretreatment
Test organisms
- Test organisms (species):
- Oncorhynchus mykiss (previous name: Salmo gairdneri)
- Details on test organisms:
- TEST ORGANISM
- Common name: Rainbow Trout
- Strain: Walbaum 1972
- Source: BASF's own breed raised from eggs of Forellenzucht Eusserthal petrihof, Eber soehne, Eusserthal
- Length at study initiation (length definition, mean, range and SD): 6.8 cm (Range 6.0-7.5)
- Weight at study initiation (mean and range, SD): 3.4 g (Range 2.4-4.4)
ACCLIMATION
- Acclimation period: -
- Acclimation conditions (same as test or not): housing conditions were the same as test conditions
- Type and amount of food: sniff spezialdiäten; Growing feed F/B 50; ad libitum
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
Test conditions
- Hardness:
- Total hardness: ca. 2.5 mmol/L
- Test temperature:
- 12-13 °C
- pH:
- 7.2
- Dissolved oxygen:
- 10.2-10.4 mg/l
- Nominal and measured concentrations:
- Nominal concentrations: 50, 100, 464, 1000, 2150, 4640 and 10000 mg/L
- 10000 mg/L was tested in parallel after pH-adjustement - Details on test conditions:
- TEST SYSTEM
- Type (delete if not applicable): glass, 100L
- Renewal rate of test solution (frequency/flow rate): not given
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates):1
- No. of vessels per vehicle control (replicates):-
- Biomass loading rate: 0.34 g/l
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:
- Total organic carbon:
- Particulate matter:
- Metals:
- Pesticides:
- Chlorine:
- Alkalinity:
- Ca/mg ratio:
- Conductivity:
- Culture medium different from test medium:
- Intervals of water quality measurement:
OTHER TEST CONDITIONS
- Adjustment of pH: only for 10000 mg/L
- Photoperiod: 16/8
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : LC50, NOEC 0, 1, 4, 24, 48, 72 and 96 h
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 2 200 - < 4 600 mg/L
- Nominal / measured:
- nominal
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 2 150 mg/L
- Nominal / measured:
- nominal
- Basis for effect:
- behaviour
- Duration:
- 1 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 10 000 mg/L
- Nominal / measured:
- nominal
- Basis for effect:
- mortality (fish)
- Duration:
- 4 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 10 000 mg/L
- Nominal / measured:
- nominal
- Basis for effect:
- mortality (fish)
- Duration:
- 24 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 4 600 - < 10 000 mg/L
- Nominal / measured:
- nominal
- Basis for effect:
- mortality (fish)
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 2 200 - < 4 600 mg/L
- Nominal / measured:
- nominal
- Basis for effect:
- mortality (fish)
- Duration:
- 72 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 2 200 - < 4 600 mg/L
- Nominal / measured:
- nominal
- Basis for effect:
- mortality (fish)
- Details on results:
- slight sedimentation at concentrations higher 2150 mg/L
- Results with reference substance (positive control):
- None used
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The substance is not toxic to the fish in concentrations under 2150 mg/L. Effects above this concentration could be prevented by pH-adjustment
- Executive summary:
To assess the acute toxicity a study according to OECD 203 was performed with Na-silicophosphonat. The concentration range was from 50 to 10000 mg/L. The control worked as expected. The LC50 (96h) was between 2200 and 4600 mg/L. The NOEC was 2150 mg/l.
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