Sodium ethylenesulphonate

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1986-09-15 to 1986-09-19

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

test procedure in accordance with national standard methods with acceptable restrictions

Remarks:

Study scientifically fully valid, but predating the implementation of GLP.

Qualifier:

equivalent or similar to guideline

Guideline:

other: DIN 38 412, June 1982

Deviations:

no

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION: the test item was added to the test water without any pre-treatment

Test organisms (species):

Leuciscus idus

Details on test organisms:

TEST ORGANISM
- Common name: Golden orfe
- Strain: Leuciscus Idus L., golden variety
- Source: Fischzucht Paul Eggers, 2354 Hohenwestedt, Germany
- Length at study initiation: 9.6 cm (range 8.1 - 11.9)
- Weight at study initiation: 9.6 g (range 7.4 - 17.1)

ACCLIMATION
- Acclimation period: 13 months
- Acclimation conditions: same as test conditions
- Type and amount of food: "FUKOSALM"-Growing Feed 1 and SSNIFF- Growing Feed F/B 50 Club, ad libitum
- Health during acclimation (any mortality observed): Mortality during the last two weeks of housing: about 0.3 %

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

2.5 mmol/L

Test temperature:

20 °C

pH:

about 7.9

Dissolved oxygen:

> 60 % of air saturation value

Nominal and measured concentrations:

Nominal: 0, 1000, 5000 and 10000 mg/L
Measured: not determined

Details on test conditions:

TEST SYSTEM
- Test vessel: glass aquarium
- Type: open
- Material, size, fill volume: glass; 30 cm x 22 cm x 24 cm; 10 L
- Aeration: continuous aeration with oil-free air
- No. of organisms per vessel: 5 resp. 10 (5 fishs at 5000 and 10000 mg/L and 10 fishs for 0 and 1000 mg/L)
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- No. of vessels per vehicle control (replicates): 1
- Biomass loading rate: 4.8 resp. 9.6 g fish/L test water

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstitued fresh water according to DIN 38412 (preparation from fully demineralized tap water)
- Na/K ratio: 10:1
- Alkalinity: 0.8 mmol/L
- Ca/Mg ratio: 4:1
- Conductivity: 10 µ MHO
- Culture medium different from test medium: no


OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 hours light/8 hours dark

EFFECT PARAMETERS MEASURED: Determination of mortality and symptoms; determination or calculation of the median lethal concentration (LC50) and, if possible, the LC5 and the LC95, using the probit analysis after hours (nominal conc.): 1, 4, 24, 48, 72, and 96 hours.


TEST CONCENTRATIONS
- Justification for concentrations than requested by guideline: In view of the objective, the requirements of the guideline and as no range finding study had been performed the concentrations were fixed at 1000, 5000 and 10000 mg/L. The concentrations exceeded the normal upper limit for acute studies in fish because it was the aim to test higher concentrations for classification of water pollution.

Reference substance (positive control):

yes

Remarks:

chloroacetamide

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

10 000 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

10 000 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

> 10 000 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mortality (fish)

Details on results:

- Behavioural abnormalities: No
- Mortality of control: No
- Abnormal responses: No

Results with reference substance (positive control):

- Mortality: yes
- LC50: after 48 hours 38 mg/L
- Other: The lethal concentration corresponds to the normal sensitivity.

Sublethal observations / clinical signs:

Table 1: Results on effect determination (mortality and symptoms)

Nominal Conc. (mg/L) Number of fish		Dead fish after
		1 h	4 h	24 h	48 h	72 h	96 h
1000	5	0	0	0	0	0	0
5000	5	0	0	0	0	0	0
10000	10	0	0	0	0	0	0
0	10	0	0	0	0	0	0
Nominal Conc. (mg/L) Number of fish		Symptoms
		1 h	4 h	24 h	48 h	72 h	96 h
1000	5	No symptoms detectable
5000	5
10000	10
0	10

Validity criteria fulfilled:

not specified

Conclusions:

The test item was tested for acute toxicity to freshwater fish in a 96h-static test. An LC50 of greater than 10000 mg/L and a NOEC of 10000 mg/L have been derived for the 25 % aqueous solution.

Executive summary:

The test item was assessed in a short-term toxicity study for fish in a method that closely followed the guideline of DIN 38 412 ", using a static procedure. Leuciscus idus, Golden orfes were exposed to the test item in a static test for 96 hours at nominal concentrations of 1000, 5000 and 10000 mg/L. No mortality occurred up to the highest tested concentration. No abnormal behaviour was observed. The LC₅₀ of > 10000 mg/L and a NOEC of 10000 mg/L have been derived for the 25 % aqueous solution.

Description of key information

The test item was tested for acute toxicity to freshwater fish in a 96h-static test. An LC₅₀ of greater than 10000 mg/L and a NOEC of 10000 mg/L have been derived for the 25 % aqueous solution.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Dose descriptor:

LC50

Effect concentration:

10 000 mg/L

Additional information

key study

The test item was assessed in a short-term toxicity study for fish in a method that closely followed the guideline of DIN 38 412 ", using a static procedure. Leuciscus idus, Golden orfes were exposed to the test item in a static test for 96 hours at nominal concentrations of 1000, 5000 and 10000 mg/L. No mortality occurred up to the highest tested concentration. No abnormal behaviour was observed. The LC₅₀ of > 10000 mg/L and a NOEC of 10000 mg/L have been derived for the test solution containing 25 % of the test substance. Based on this study, the derived LC50 and NOEC for 100 % of the test item were > 2500 mg/L and 2500 mg/L, respectively.

 

supporting study

Sodium vinylsulphonate was assessed in a short-term toxicity study with fish according to OECD guideline 203. Guppies were exposed to the test item for 24 hours. The EC0 was greater than 500 mg/L based on the lack of mortality and sublethal effects at this concentration and lower for the 30 % test solution.