2,4,6-tris(2,4,6-tribromophenoxy)-1,3,5-triazine

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP and appropriate guidelines

Cross-reference

Reason / purpose for cross-reference:

reference to same study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1997

Report date:

1997

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

other: method B14 of the commission directive 92/69/EEC (annex V of council directive 67/548/EEC)

Test guideline 2

Qualifier:

according to guideline

Guideline:

OECD Guideline 471 (Bacterial Reverse Mutation Assay)

Principles of method if other than guideline:

not relevant

GLP compliance:

yes

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

2,4,6-tris(2,4,6-tribromophenoxy)-1,3,5-triazine

EC Number:

426-040-2

EC Name:

2,4,6-tris(2,4,6-tribromophenoxy)-1,3,5-triazine

Cas Number:

25713-60-4

Molecular formula:

C 21 H 6 Br 9 N 3 O 3

IUPAC Name:

tris(2,4,6-tribromophenoxy)-1,3,5-triazine

Constituent 2

Reference substance name:

4260402

IUPAC Name:

4260402

Details on test material:

Identification SR-245
Description White Powder
Batch 969066
Purity 100%
Test substance storage At room temperature in the dark
Stability under storage conditions Stable

Method

Target gene:

Histidine

Species / strain

Species / strain / cell type:

bacteria, other: Salmonella typhimurium TA1535, TA1537, TA98, TA100

Details on mammalian cell type (if applicable):

not relevant

Additional strain / cell type characteristics:

other: Strains TA98 and TA1537 were capable of detecting frameshift mutagens, strains TA100 and TA1535 are capable of detecting base-pair substitution mutagens

Metabolic activation system:

Aroclor-induced rat liver S9.

Test concentrations with justification for top dose:

Concentration range in the dose range finding test (with metabolic activation): 3, 10, 33, 100, 333, 1000, 3300, 5000 µg/plate
Concentration range in the dose range finding test (without metabolic activation): 3, 10, 33, 100, 333, 1000, 3300, 5000 µg/plate
Concentration range in the main test (without metabolic activation): 10, 33, 100, 333, 1000 µg/plate
Concentration range in the main test (without metabolic activation): 10, 33, 100, 333, 1000 µg/plate

Vehicle / solvent:

Solvent: Dimethylsulphoxide

Details on test system and experimental conditions:

See attached document of test system

Evaluation criteria:

Test substance is considered negative (not mutagenic) in the test if:
1. The total number of revertants in any tester strain at any concentration is not greater than two times the solvent control value, with or without metabolic activation.
2. The negative response should be reproducible in at least one independently repeated experiment.
Test substance is considered positive (mutagenic) in the test if:
1. It induces at least a 2-fold, dose related increase in the number of revertants with respect to the number induced by the solvent control in any of the tester strains, either with or without metabolic activation. However, any mean plate count of less than 20 is considered to be not significant.
2. The positive response should be reproducible in at least one independently repeated experiment.

The preceding criteria were not absolute and other modifying factors might enter into the final evaluation decision.

Statistics:

See evaluation criteria

Results and discussion

Test results

Test results 1

Species / strain:

S. typhimurium TA 1535, TA 1537, TA 98 and TA 100

Metabolic activation:

with and without

Genotoxicity:

negative

Remarks:

(> 5000 µg/plate)

Cytotoxicity / choice of top concentrations:

no cytotoxicity

Remarks:

(> 5000 µg/plate)

Vehicle controls validity:

valid

Positive controls validity:

valid

Test results 2

Species / strain:

S. typhimurium TA 1535, TA 1537, TA 98 and TA 100

Metabolic activation:

with and without

Genotoxicity:

negative

Remarks:

(> 1000 µg/plate)

Cytotoxicity / choice of top concentrations:

no cytotoxicity

Remarks:

(> 1000 µg/plate)

Vehicle controls validity:

valid

Positive controls validity:

valid

Additional information on results:

see attached document on results

Remarks on result:

other: other: dose range finding test

Remarks:

Migrated from field 'Test system'.

Any other information on results incl. tables

See attached file on tables and figures

Applicant's summary and conclusion

Conclusions:

Interpretation of results (migrated information):
negative with metabolic activation SR-245 did not induce a dose related increase in the number of revertant colonies in the presence of S9-mix
negative without metabolic activation SR-245 did not induce a dose related increase in the number of revertant colonies in the absence of S9-mix

Based on the results of the study it is concluded that SR-245 is not mutagenic in the Salmonella typhimurium reverse mutation assay.