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Toxicological information

Acute Toxicity: dermal

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Administrative data

Endpoint:
acute toxicity: dermal
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: The published literature fulfilled basically scientific principles.
Justification for data waiving:
other:

Data source

Reference
Reference Type:
publication
Title:
Unnamed
Year:
2001

Materials and methods

Test guideline
Qualifier:
no guideline required
Principles of method if other than guideline:
In order to study at the ultrastructural level which part of the skin is associated with percutaneous iodide transport by passive diffusion, the iodide (KI solution) was applied to the frozen skin of human. Then by the transmission electron microscopy (TEM) and X-ray microanalysis (XRMA), the distribution of iodide in each layer of skin after passive diffusion can be well investigated.
GLP compliance:
no
Remarks:
Publication
Test type:
other: In vitro percutaneous penetration test
Limit test:
no

Test material

Constituent 1
Reference substance name:
iodide in KI
IUPAC Name:
iodide in KI
Details on test material:
potassium iodide (KI) was from Difco Laboratories (Detroit, MI).

KI-Agar Gel Standards
To obtain KI-agar gels containing 0.0, 0.056, 0.11, 0.56, and 1.1 M KI, first a 7% (w/w) solution of agar was heated up to 100°C. Then this solution was cooled to 50°C and subsequently mixed with the same volume of a KI solution of a known concentration (containing 0.1% (w/w) Na2S2O3) so that the desired concentration KI in gel was obtained. Finally the KI-agar solution was allowed to gel at 4°C.

Test animals

Species:
other: in vitro human skin
Strain:
not specified
Sex:
not specified

Administration / exposure

Type of coverage:
other: The stratum corneum side was exposed to a 1.1 M KI solution
Vehicle:
water
Remarks:
1.1 M KI solution
Duration of exposure:
10 h
Doses:
1.1 M KI solution
No. of animals per sex per dose:
Irrelevant
Control animals:
not required

Results and discussion

Any other information on results incl. tables

After passive diffusion, iodide was mainly found in the stratum corneum (SC), whereas there was little iodide in the viable epidermis.

Applicant's summary and conclusion

Interpretation of results:
other: The dermal absorption is negligible
Remarks:
Criteria used for interpretation of results: other: irrelevant
Conclusions:
Iodide was mainly found in the stratum corneum (SC), whereas there was little iodide in the viable epidermis.
Executive summary:

In order to study at the ultrastructural level which part of the skin is associated with percutaneous iodide transport by passive diffusion, the iodide (KI solution) was applied to the frozen skin of human. Then by the transmission electron microscopy (TEM) and X-ray microanalysis (XRMA), the distribution of iodide in each layer of skin after passive diffusion can be well investigated. It concluded that the iodide can penetrate into stratum corneum (SC), but little can enter viable epidermis.