Reaction product of {mixture of [poly(1~4)chlorosulfonylphthalocyaninato-N29,N30,N31,N32]copper(Ⅱ) and [poly(1~3) chlorosulfonyl (tribenzo[b,g,l]pyrido[2,3-q]-5,10,15,20-tetraazaporphyrinato-N21,N22,N23,N24)] copper(Ⅱ) and [poly(1~2) chlorosulfonyl (dibenzo[b,g(or b,l)]dipyrido [2,3(or 3,2)-l,q(or g,q)]-5,10,15,20-tetraazaporphyrinato-N21,N22,N23,N24)] copper(Ⅱ) and [monochlorosulfonyl (benzo[b]tripyrido [2,3(or 3,2)-g,l,q]-5,10,15,20-tetraazaporphyrinato-N21,N22,N23,N24)]copper(Ⅱ) }and 2-[4-(2-aminoethylamino)-6-(benzylamino)-1,3,5-triazin-2-ylamino]benzene-1,4-disulfonic acid, and ammonia water and sodium chloride

Adsorption / desorption

Link to relevant study record(s)

Reference

Endpoint:

adsorption / desorption: screening

Type of information:

experimental study

Adequacy of study:

key study

Study period:

11 February 2011 – 27 April 2011

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: This study has been performed according to OECD and EC guidelines and in compliance with GLP principles.

Qualifier:

according to guideline

Guideline:

OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))

Version / remarks:

(2001)

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method C.19 (Estimation of the Adsorption Coefficient (KOC) on Soil and Sewage Sludge Using High Performance Liquid Chromatography (HPLC))

Version / remarks:

(Official Journal of the European Union no. L142, May 31, 2008)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Type of method:

HPLC estimation method

Media:

soil/sewage sludge

Radiolabelling:

no

Test temperature:

35°C +/- 1°C

Details on study design: HPLC method:

Analytical conditions
Instrument: Alliance Separation Module 2695 (Waters, Milford, MA, USA)
Detector: Dual λ Absorbance Detector 2487 (Waters)
Column: Hypersil BDS-CN, 150 mm x 4.6 mm i.d., dp = 5 μm (Thermo Fisher Scientific, Waltham, MA, USA)
Column temperature: 35°C +/- 1°
Mobile phase: 55/45 (v/v) methanol/water
Flow: 1 ml/min
Injection volume: 10 μl
UV detection: 210 nm

E-C104 is a complex mixture of a range of poly substituted organic salt compounds. Therefore, the test was performed at neutral pH.

Type:

Koc

Value:

< 18 dimensionless

Temp.:

35 °C

Remarks on result:

other: HPLC estimation method

Key result

Type:

log Koc

Value:

< 1.3 dimensionless

Temp.:

35 °C

Remarks on result:

other: HPLC estimation method

Details on results (HPLC method):

In the chromatogram of the test substance solution, one peak with a retention time of 1.11 minutes was observed. Under the same analytical conditions, the retention time of acetanilide (log Koc = 1.26) was 2.28 minutes. Hence, it was concluded that the log Koc of test substance was < 1.3 (Koc < 18).

Validity criteria fulfilled:

yes

Conclusions:

The HPLC method using soil-adsorption-reference data was applied for the determination of the adsorption coefficient (Koc) of E-C104.
The Koc and log Koc values of the test substance at neutral pH were < 18 and < 1.3, respectively.

Key value for chemical safety assessment

Koc at 20 °C:

18