Perhydropyrimidin-2-one

Toxicity to soil macroorganisms except arthropods

Administrative data

Endpoint:

toxicity to soil macroorganisms except arthropods: short-term

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2001

Report date:

2001

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 207 (Earthworm, Acute Toxicity Tests)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

2-imidazolidone

EC Number:

204-436-4

EC Name:

2-imidazolidone

Cas Number:

120-93-4

IUPAC Name:

imidazolidin-2-one

Details on test material:

- Name of test material (as cited in study report): BF 222-EU
- IUPAC-name: Imidazolidine-2-one
- Batch No.: 27270
- Analytical purity: 88.6 %

Test organisms

Test organisms (species):

Eisenia fetida

Animal group:

annelids

Details on test organisms:

TEST ORGANISM
- Common name: Earthworms
- Source: From in-house culture
- Age at test initiation: Adult animals with clitellum, less then one year old
- Weight at test initiation: Between 250 and 600 mg

ACCLIMATION
- Acclimation conditions: Before being used in the test, the animals were kept in boxes an fed with horse manure.
- Adaptation: About 24 hours to artificial soil

Study design

Study type:

laboratory study

Substrate type:

artificial soil

Limit test:

no

Total exposure duration:

14 d

Test conditions

Test temperature:

20 - 21 °C

pH:

5.8

Moisture:

water contant (dry weight):
at test initiation 34.8 g/100 g soil
at test termination 32.5 g/100 g soil

Details on test conditions:

TEST SYSTEM
- Test container: 1 L preservation jars (glass)
- Amount of soil or substrate: 750 g wet weight
- No. of organisms per container: 10
- No. of replicates per treatment group: 4
- No. of replicates per control: 4

SOURCE AND PROPERTIES OF SUBSTRATE
- Artificial soil according to OECD 207:
69 % Quarz sand (Fa. Gebr. Willersinn, Particle size 0.063 - 0.2 mm)
20 % Kaolin (BASF Kaolin B22)
10 % Sphagnum peat (Gartengold Order No. 14030, DIN 11540-F250)
1% CaCO3 (Riedel-de Häen, Order No. 12010)

OTHER TEST CONDITIONS
- Photoperiod: continuous illumination

EFFECT PARAMETERS MEASURED:
After 7 days worm mortality was assessed by emptying the glass containers onto suitable trays and estimating the survived worms. Additionally their reaction to mechanical stimulus and other anormalities were observed. After 14 days the same assessment was done and additionally the live weight of the worm was determined after gentle washing and drying.

Nominal and measured concentrations:

Test concentrations (dry weight) nominal based on the batch (88.6 % purity): 0, 197.5, 296.3, 444.4, 666.7 and 1000 mg/kg
Test concentrations (dry weight) actual based on calculation (100 % purity): 0, 175, 262.5, 393.7, 590.7 and 886 mg/kg

Reference substance (positive control):

yes

Remarks:

Chloracetamide

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

14 d

Dose descriptor:

LC50

Effect conc.:

> 886 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Effect concentrations 2

Duration:

14 d

Dose descriptor:

other: NOAEC

Effect conc.:

> 886 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: mortality and biomass

Details on results:

The substance caused no mortality on earthworm even in the highest concentration. Neither a reduction in worm biomass could be measured. In contrary the biomass rised in all variants especially in the treatement groups with higher test concentrations. No other particular behavioural or morphological changes were observed.