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EC number: 266-945-8 | CAS number: 67701-27-3 This substance is identified by SDA Substance Name: C14-C18 trialkyl glyceride and SDA Reporting Number: 17-001-00.
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- migrated information: read-across based on grouping of substances (category approach)
- Adequacy of study:
- key study
- Study period:
- May 12th, 1987 - June 22nd, 1987
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Basic data given
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 987
- Report date:
- 1987
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- yes
- Remarks:
- : only 3 Salmonella strains tested,
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Glycerides, C12-18
- EC Number:
- 266-944-2
- EC Name:
- Glycerides, C12-18
- Cas Number:
- 67701-26-2
- IUPAC Name:
- 67701-26-2
- Details on test material:
- - Name of test material (as cited in study report): C10-18 triglycerides
- Name of test material: Hydrogenated Coco-Glycerides
- Storage condition of test material: desiccated at room temperature in the dark
- Analytical purity: not stated for the tested compound
- Physical state: white pellets
Constituent 1
Method
- Target gene:
- His-Operon
Species / strain
- Species / strain / cell type:
- S. typhimurium, other: TA97, TA98 and TA100
- Metabolic activation:
- with and without
- Metabolic activation system:
- Aroclor-1254 induced rat liver S9 mix
- Test concentrations with justification for top dose:
- 0, 1.6, 8, 40, 200, 1000 µg/plate
- Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: Ethylene glycol dimethyl ether (EGDME)
Controls
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- Remarks:
- EGDME
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- Remarks:
- Migrated to IUCLID6: 2-nitrofluorene (TA98, 50 µg/plate); sodium azide (TA100, 2 µg/plate); 9-aminoacridine (TA97, 50 µg/plate); 2-aminoanthracene (all strains, 5 µg/plate)
- Details on test system and experimental conditions:
- METHOD OF APPLICATION:
in medium; in agar (plate incorporation);
DURATION
- Exposure duration: 48 h
NUMBER OF REPLICATIONS: 1 experiment with triplicate plating
DETERMINATION OF CYTOTOXICITY
- Method: thinning of background layer and/or presence of microcolonies - Evaluation criteria:
- At least 2-fold increase of revertant numbers compared to controls and dose-response correlation.
- Statistics:
- Analysis of variance and regression analysis.
Results and discussion
Test results
- Species / strain:
- S. typhimurium, other: TA97, TA98 and TA100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity, but tested up to precipitating concentrations
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Additional information on results:
- TEST-SPECIFIC CONFOUNDING FACTORS
- Water solubility: limited solubility at 1000 µg/plate, but at this dose the precipitated test agent did not interfere with the counting of colonies. Thus 1000 µg/plate was chosen as the highest concentration fur mutation testing.
- Precipitation: yes, at 1000 µg/plate
RANGE-FINDING/SCREENING STUDIES:
Stain TA100 was used for testing up to 5000 µg/plate.
COMPARISON WITH HISTORICAL CONTROL DATA: yes
ADDITIONAL INFORMATION ON CYTOTOXICITY:
No toxicity was observed in TA100 in the absence or presence of S9 mix. At 5000 µg/plate precipitation occured.
Any other information on results incl. tables
Table 1: Ames Test Results after treatment with C10-18 triglycerides
With or without S9-Mix |
Test substance concentration (μg/plate) |
Mean number of revertant colonies per plate (average of 3 plates) |
||
TA97 |
TA98 |
TA100 |
||
- |
0 (vehicle) |
96.0 |
31.6 |
120.0 |
- |
1.6 |
80.7 |
21.7 |
131.3 |
- |
8 |
103.3 |
23.7 |
117.5 |
- |
40 |
88.3 |
17.0 |
141.0 |
- |
200 |
94.0 |
23.3 |
144.3 |
- |
1000 |
92.7ST |
16.0P |
124.0P |
Positive controls - S9 |
Name |
9AA |
2NF |
SA |
Concentrations (μg/plate) |
50 |
50 |
2 |
|
Number of colonies/plate |
446.4 |
920.4 |
886.8 |
|
+ |
0 (vehicle) |
132.2 |
46.8 |
138.8 |
+ |
1.6 |
145.0 |
34.3 |
152.7 |
+ |
8 |
152.0 |
36.7 |
140.3 |
+ |
40 |
165.7 |
37.0 |
141.3 |
+ |
200 |
142.3 |
44.7 |
143.7 |
+ |
1000 |
142.0P |
52.3P |
139.7P |
Positive controls + S9 |
Name |
2AA |
2AA |
2AA |
Concentrations (μg/plate) |
5 |
5 |
5 |
|
Number of colonies/plate |
499.8 |
689.2 |
888.4 |
2AA = 2-aminoanthracene
2NF = 2-nitrofluorene
SA = sodium azide
9AA = 9-aminoacridine
P= Precipitation observed
ST= signs of toxicity observed (thinning of background layer and/or presence of microcolonies)
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative in TA97, TA98 and TA100
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